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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Fatty Acid Synthase 抗体 [EPR7465] (ab128856)

Reviews (5)Q&A (1)References (21)
Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
  • Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
  • Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
  • Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
  • Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7465] to Fatty Acid Synthase
  • Suitable for: WB, ICC/IF, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 APC Carrier Free PE

製品の概要

  • 製品名

    Anti-Fatty Acid Synthase antibody [EPR7465]
    Fatty Acid Synthase 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR7465] to Fatty Acid Synthase

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IF, Flow Cyt (Intra)more details
    適用なし: IHC-P or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human Fatty Acid Synthase aa 1-100. The exact sequence is proprietary.
    Database link: P49327

  • ポジティブ･コントロール

    • WB: HeLa, HEK-293, 293T, A549, SHSY-5Y, NIH/3T3, L6 (Rat skeletal muscle myoblast) and MOLT4 cell lysates; Mouse brain, Rat brain lysates. ICC/IF: A549 cells. IHC-Fr: Human peritoneal tumor tissue. Flow Cyt (intra): A549 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab128856の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (2)
1/1000 - 1/10000. Predicted molecular weight: 273 kDa.
ICC/IF (1)
1/50.

For unpurified version, use at 1/250 - 1/500 dilution.
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 273 kDa.
ICC/IF
1/50.

For unpurified version, use at 1/250 - 1/500 dilution.
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
追加情報
Is unsuitable for IHC-P or IP.

ターゲット情報

  • 機能

    Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities and an acyl carrier protein.

  • 組織特異性

    Ubiquitous. Prominent expression in brain, lung, and liver.

  • 配列類似性

    Contains 1 acyl carrier domain.

  • 細胞内局在

    Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • [Acyl-carrier-protein] S acetyltransferase antibody
    • [Acyl-carrier-protein] S malonyltransferase antibody
    • 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase antibody
    • 3-oxoacyl-[acyl-carrier-protein] reductase antibody
    • 3-oxoacyl-[acyl-carrier-protein] synthase antibody
    • Enoyl-[acyl-carrier-protein] reductase antibody
    • FAS antibody
    • FAS_HUMAN antibody
    • FASN antibody
    • Fatty acid synthase antibody
    • MGC14367 antibody
    • MGC15706 antibody
    • OA 519 antibody
    • Oleoyl-[acyl-carrier-protein] hydrolase antibody
    • SDR27X1 antibody
    • Short chain dehydrogenase/reductase family 27X member 1 antibody
    see all

画像

  • Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    All lanes : Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856) at 1/5000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 3 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
    Lane 4 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate
    Lane 5 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
    Lane 6 : Mouse brain lysate
    Lane 7 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 8 : Rat brain lysate
    Lane 9 : L6 (Rat skeletal muscle myoblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 273 kDa
    Observed band size: 273 kDa

  • Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labelling Fatty Acid Synthase with Purified ab128856 at 1:50 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Fatty Acid Synthase with Purified ab128856 at 1:50 dilution (4.5 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with . Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Fatty Acid Synthase knockout HAP1 cell lysate (20 µg)
    Lane 3: A549 cell lysate (20 µg)
    Lane 4: Hu liver tissue lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab128856 observed at 250 kDa. Red - loading control, ab18058, observed at 124 kDa.

    ab128856 was shown to specifically react with Fatty Acid Synthase in wild-type HAP1 cells. No band was observed when Fatty Acid Synthase knockout samples were examined. Wild-type and Fatty Acid Synthase knockout samples were subjected to SDS-PAGE. ab128856 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
    Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)

参考文献 (21)

ab128856 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab128856 は 21 報の論文で使用されています。

  • Yang Z  et al. Hepatic DKK1-driven steatosis is CD36 dependent. Life Sci Alliance 6:N/A (2023). PubMed: 36410795
  • Huang SM  et al. Antidiabetic and antihyperlipidemic activities of Phyllanthus emblica L. extract in vitro and the regulation of Akt phosphorylation, gluconeogenesis, and peroxisome proliferator-activated receptor α in streptozotocin-induced diabetic mice. Food Nutr Res 67:N/A (2023). PubMed: 37850072
  • Zhang N  et al. Silencing of CD147 inhibits cell proliferation, migration, invasion, lipid metabolism dysregulation and promotes apoptosis in lung adenocarcinoma via blocking the Rap1 signaling pathway. Respir Res 24:253 (2023). PubMed: 37880644
  • Gong TQ  et al. Proteome-centric cross-omics characterization and integrated network analyses of triple-negative breast cancer. Cell Rep 38:110460 (2022). PubMed: 35235781
  • Rahbani JF  et al. Creatine kinase B controls futile creatine cycling in thermogenic fat. Nature 590:480-485 (2021). PubMed: 33597756
View all Publications for this product

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1-6 of 6 Abreviews or Q&A

Flow Cytometry abreview for Anti-Fatty Acid Synthase antibody [EPR7465]

 Good
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (Peripheral blood mononuclear cells)
Permeabilization
Yes - Methanol
Gating Strategy
Live cells.
Specification
Peripheral blood mononuclear cells
Fixation
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 19 2018

Western blot abreview for Anti-Fatty Acid Synthase antibody [EPR7465]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (rat liver)
Gel Running Conditions
Reduced Denaturing (7% SDS-PAGE)
Loading amount
20 µg
Specification
rat liver
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 05 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-Fatty Acid Synthase antibody [EPR7465]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse Cell (Tumor))
Permeabilization
Yes - 0.3% triton x100
Specification
Mouse Cell (Tumor)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 05 2016

Immunohistochemistry (Frozen sections) abreview for Anti-Fatty Acid Synthase antibody [EPR7465]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Sample
Human Tissue sections (Peritoneal tumor)
Specification
Peritoneal tumor
Permeabilization
No
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 14 2014

Western blot abreview for Anti-Fatty Acid Synthase antibody [EPR7465]

 Excellent
Abreviews
abreview image
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (8% GEL, SDS PAGE)
Sample
Human Cell lysate - other (LS180)
Specification
LS180
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 01 2013

Question

Customer kindly contacted us regarding use of this product in Musca domestica.

Read More
Answer

Thank you for contacting us.

I have reviewed the immunogen information for each of our anti-Fatty Acid Synthase antibodies. Unfortunately none have homologies which would guarantee they could work in Musca domestica, however ab128856 Rabbit monoclonal [EPR7465] to Fatty Acid Synthase does have an 87% positive identity score. Further the identical residues are grouped, which would be necessary for epitope recognition. Since the product has not yet been tested in house fly, we cannot guarantee that it will work, however, for UNTESTED applications; we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount equal to the value of the product tested. The terms and conditions of this offer can be found here: https://www.abcam.com/AbTrial


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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