Anti-Fascin 抗体 (ab183891)
Key features and details
- Rabbit polyclonal to Fascin
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Fascin antibody
Fascin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Fascin -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment within Human Fascin aa 89-365. The exact sequence is proprietary.
Database link: Q16658 -
ポジティブ･コントロール
- Mouse ovary tissue, HCT116 cells, HeLa whole cell lysate/extract
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab183891の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 54 kDa.
|
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| ICC/IF |
1/100 - 1/1000.
|
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| IHC-P |
1/100 - 1/1000.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 54 kDa. |
|
ICC/IF
1/100 - 1/1000. |
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IHC-P
1/100 - 1/1000. |
ターゲット情報
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機能
Organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the fascin family. -
ドメイン
Composed of four beta-trefoil domains. -
翻訳後修飾
Phosphorylation on Ser-39 inhibits the actin-binding ability of fascin. -
細胞内局在
Cytoplasm > cytoskeleton. Cell projection > filopodium. Cell projection > invadopodium. - Information by UniProt
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参照データベース
- Entrez Gene: 6624 Human
- Entrez Gene: 14086 Mouse
- Omim: 602689 Human
- SwissProt: Q16658 Human
- SwissProt: Q61553 Mouse
- Unigene: 118400 Human
- Unigene: 289707 Mouse
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別名
- 55 kDa actin bundling protein antibody
- 55 kDa actin-bundling protein antibody
- Actin bundling protein antibody
see all
画像
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Western blot - Anti-Fascin antibody (ab183891)All lanes : Anti-Fascin antibody (ab183891) at 1/2000 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Fascin Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 54 kDa -
Western blot - Anti-Fascin antibody (ab183891)Anti-Fascin antibody (ab183891) at 1/1000 dilution + HeLa whole cell lysate/extract at 30 µg
Predicted band size: 54 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Fascin antibody (ab183891)Immunofluorescence analysis of HCT116 cells fixed in 100% methanol for 5 minutes, labeling Fascin using ab183891 at a 1/500 dilution (green). Blue: Hoechst 33342 staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody (ab183891)Immunohistochemical analysis of paraffin-embedded mouse ovary tissue, labeling Fascin using ab183891 at a 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab183891 は 4 報の論文で使用されています。
- Gupta I et al. Oncoproteins of High-Risk HPV and EBV Cooperate to Enhance Cell Motility and Invasion of Human Breast Cancer Cells via Erk1/Erk2 and ß-Catenin Signaling Pathways. Front Oncol 11:630408 (2021). PubMed: 33777781
- Jabeen A et al. Elaeagnus angustifolia Plant Extract Inhibits Epithelial-Mesenchymal Transition and Induces Apoptosis via HER2 Inactivation and JNK Pathway in HER2-Positive Breast Cancer Cells. Molecules 25:N/A (2020). PubMed: 32947764
- Hua X et al. LSAMP-AS1 binds to microRNA-183-5p to suppress the progression of prostate cancer by up-regulating the tumor suppressor DCN. EBioMedicine 50:178-190 (2019). PubMed: 31727599
- Hao YJ et al. Role of RNA-interference-induced zinc finger protein 139 suppression in gastric cancer cell sensitivity to chemotherapeutic agents. Oncol Lett 10:1333-1338 (2015). WB . PubMed: 26622672