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リコンビナントRabMAb

Anti-Fas Ligand 抗体 [EPR25843-87] (BSA and Azide free) (ab302906)

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Western blot - Anti-Fas Ligand antibody [EPR25843-87] (BSA and Azide free) (AB302906)
  • Western blot - Anti-Fas Ligand antibody [EPR25843-87] (BSA and Azide free) (AB302906)
  • Immunoprecipitation - Anti-Fas Ligand antibody [EPR25843-87] (BSA and Azide free) (AB302906)
  • Anti-Fas Ligand antibody [EPR25843-87] (BSA and Azide free) (AB302906)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25843-87] to Fas Ligand - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Fas Ligand antibody [EPR25843-87] (BSA and Azide free)
    Fas Ligand 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25843-87] to Fas Ligand - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details
    適用なし: Flow Cyt (Intra),ICC/IF or IHC-P

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Jurkat and No-GFP-CD16.NK-92 (natural killer (NK-92®) cell lacking GFP-CD16) treated with TPA and Ionomycin whole cell lysates. IP: No-GFP-CD16.NK-92 (natural killer (NK-92®) cell lacking GFP-CD16) treated with TPA and Ionomycin whole cell lysate.

  • 特記事項

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302906の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 31 kDa).
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 31 kDa).
IP
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt (Intra),ICC/IF or IHC-P.

ターゲット情報

  • 機能

    Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.

  • 関連疾患

    Defects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.

  • 配列類似性

    Belongs to the tumor necrosis factor family.

  • 翻訳後修飾

    N-glycosylated.
    The soluble form derives from the membrane form by proteolytic processing.

  • 細胞内局在

    Cell membrane. Secreted. May be released into the extracellular fluid, probably by cleavage form the cell surface.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ALPS1B antibody
      • Apoptosis (APO 1) antigen ligand 1 antibody
      • Apoptosis antigen ligand 1 antibody
      • Apoptosis antigen ligand antibody
      • APT1LG1 antibody
      • APTL antibody
      • CD178 antibody
      • CD178 antigen antibody
      • CD95 ligand antibody
      • CD95-L antibody
      • CD95L antibody
      • CD95L protein antibody
      • Fas antigen ligand antibody
      • Fas L antibody
      • Fas ligand (TNF superfamily member 6) antibody
      • Fas ligand antibody
      • FASL antibody
      • Fasl Fas ligand (TNF superfamily member 6) antibody
      • FASLG antibody
      • Generalized lymphoproliferative disease antibody
      • Gld antibody
      • soluble form antibody
      • TNFL6_HUMAN antibody
      • TNFSF6 antibody
      • Tumor necrosis factor (ligand) superfamily member 6 antibody
      • Tumor necrosis factor ligand superfamily member 6 antibody
      see all

    画像

    • Western blot - Anti-Fas Ligand antibody [EPR25843-87] (BSA and Azide free) (AB302906)
      Western blot - Anti-Fas Ligand antibody [EPR25843-87] (BSA and Azide free) (AB302906)
      All lanes : Anti-Fas Ligand antibody [EPR25843-87] (ab302905) at 1/1000 dilution

      Lane 1 : Untreated No-GFP-CD16.NK-92 (natural killer (NK-92®) cell lacking GFP-CD16) whole cell lysate
      Lane 2 : No-GFP-CD16.NK-92 treated with 80nM TPA (ab120297) and 3µM Ionomycin (ab120116) for 5 hours, was then added for an additional 300ng/ml BFA for last 4 hours whole cell lysate

      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 31 kDa
      Observed band size: 37 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using ab302905, the same antibody clone in a different buffer formulation.

      Blocking / Diluting buffer and concentration: 5% NFDM/TBST

    • Western blot - Anti-Fas Ligand antibody [EPR25843-87] (BSA and Azide free) (AB302906)
      Western blot - Anti-Fas Ligand antibody [EPR25843-87] (BSA and Azide free) (AB302906)
      All lanes : Anti-Fas Ligand antibody [EPR25843-87] (ab302905) at 1/1000 dilution

      Lane 1 : Untreated Jurkat (human T cell leukemia T lymphocyte from peripheral blood), whole cell lysate
      Lane 2 : Jurkat treated with 80nM TPA (ab120297) and 3µM Ionomycin (ab120116) for 5 hours, was then added for an additional 300ng/ml BFA for last 4 hours, whole cell lysate

      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 31 kDa
      Observed band size: 37 kDa why is the actual band size different from the predicted?


      Exposure time: 180 seconds


      This data was developed using ab302905, the same antibody clone in a different buffer formulation.

      Blocking / Diluting buffer and concentration: 5% NFDM/TBST

    • Immunoprecipitation - Anti-Fas Ligand antibody [EPR25843-87] (BSA and Azide free) (AB302906)
      Immunoprecipitation - Anti-Fas Ligand antibody [EPR25843-87] (BSA and Azide free) (AB302906)

      This data was developed using ab302905, the same antibody clone in a different buffer formulation.

      Fas Ligand was immunoprecipitated from 0.35 mg No-GFP-CD16.NK-92 (natural killer (NK-92®) cell lacking GFP-CD16) whole cell lysate. Cells were treated with 80nM TPA (ab120297) and 3µM Ionomycin (ab120116) for 5 hours, and then 300ng/ml BFA was added for an additional 4 hours. ab302905 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302905 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

      Lane 1: No-GFP-CD16.NK-92 (natural killer (NK-92®) cell lacking GFP-CD16) treated with 80nM TPA (ab120297) and 3µM Ionomycin (ab120116) for 5 hours, was then added for an additional 300ng/ml BFA for the last 4 hours whole cell lysate 10 µg

      Lane 2: ab302905 IP in No-GFP-CD16.NK-92 treated with 80nM TPA (ab120297) and 3µM Ionomycin (ab120116) for 5 hours, was then added for an additional 300ng/ml BFA for the last 4 hours whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302905 in No-GFP-CD16.NK-92 treated with 80nM TPA (ab120297) and 3µM Ionomycin (ab120116) for 5 hours, was then added for an additional 300ng/ml BFA for the last 4 hours whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 180 seconds

    • Anti-Fas Ligand antibody [EPR25843-87] (BSA and Azide free) (AB302906)
      Anti-Fas Ligand antibody [EPR25843-87] (BSA and Azide free) (AB302906)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab302906 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab302906 は論文での使用が確認できていません。

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