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リコンビナントRabMAb

Anti-FAS Ligand 抗体 [EPR25843-27] - BSA and Azide free (Capture) (ab300690)

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Sandwich ELISA - Anti-FAS Ligand antibody [EPR25843-27] - BSA and Azide free (Capture) (ab300690)
  • Anti-FAS Ligand [EPR25843-27] - BSA and Azide free (Capture) (AB300690)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25843-27] to Fas Ligand - BSA and Azide free (Capture)
  • Suitable for: Sandwich ELISA
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-FAS Ligand antibody [EPR25843-27] - BSA and Azide free (Capture)
    Fas Ligand 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25843-27] to Fas Ligand - BSA and Azide free (Capture)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Sandwich ELISAmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 特記事項

    ab300690 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify human FAS Ligand. The recommended pair for sandwich ELISA is:
         Capture: ab300690, human FAS Ligand Capture Antibody (unconjugated)
         Detector: ab300691, human FAS Ligand Detector Antibody (unconjugated)
    The reference range value is 15.6-1000pg/ml.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300690の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR25843-59] to Fas Ligand - BSA and Azide free (Detector) (ab300691).
特記事項
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR25843-59] to Fas Ligand - BSA and Azide free (Detector) (ab300691).

ターゲット情報

  • 機能

    Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects.

  • 関連疾患

    Defects in FASLG are the cause of autoimmune lymphoproliferative syndrome type 1B (ALPS1B) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.

  • 配列類似性

    Belongs to the tumor necrosis factor family.

  • 翻訳後修飾

    N-glycosylated.
    The soluble form derives from the membrane form by proteolytic processing.

  • 細胞内局在

    Cell membrane. Secreted. May be released into the extracellular fluid, probably by cleavage form the cell surface.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ALPS1B antibody
      • Apoptosis (APO 1) antigen ligand 1 antibody
      • Apoptosis antigen ligand 1 antibody
      • Apoptosis antigen ligand antibody
      • APT1LG1 antibody
      • APTL antibody
      • CD178 antibody
      • CD178 antigen antibody
      • CD95 ligand antibody
      • CD95-L antibody
      • CD95L antibody
      • CD95L protein antibody
      • Fas antigen ligand antibody
      • Fas L antibody
      • Fas ligand (TNF superfamily member 6) antibody
      • Fas ligand antibody
      • FASL antibody
      • Fasl Fas ligand (TNF superfamily member 6) antibody
      • FASLG antibody
      • Generalized lymphoproliferative disease antibody
      • Gld antibody
      • soluble form antibody
      • TNFL6_HUMAN antibody
      • TNFSF6 antibody
      • Tumor necrosis factor (ligand) superfamily member 6 antibody
      • Tumor necrosis factor ligand superfamily member 6 antibody
      see all

    画像

    • Sandwich ELISA - Anti-FAS Ligand antibody [EPR25843-27] - BSA and Azide free (Capture) (ab300690)
      Sandwich ELISA - Anti-FAS Ligand antibody [EPR25843-27] - BSA and Azide free (Capture) (ab300690)
      Sandwich ELISA of ab300689 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL
    • Anti-FAS Ligand [EPR25843-27] - BSA and Azide free (Capture) (AB300690)
      Anti-FAS Ligand [EPR25843-27] - BSA and Azide free (Capture) (AB300690)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300690 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300690 は論文での使用が確認できていません。

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