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AB307591

Anti-Fas 抗体 [EPR27359-51]

Anti-Fas antibody [EPR27359-51]

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Anti-Fas antibody [EPR27359-51] is a Rabbit Monoclonal antibody that is used in Fas Flow Cytometry. Suitable for Human samples.

FAS, also known as CD95, is a cell surface receptor that plays a critical role in inducing apoptosis in cancer cells. Research has shown that FAS signaling can trigger both apoptotic and non-apoptotic pathways, influencing tumor progression and immune evasion. The dual role of FAS in promoting cell death and proliferation makes it a significant focus in cancer therapy development.

別名を表示する

CD95, APT1, FAS1, TNFRSF6, FAS, Tumor necrosis factor receptor superfamily member 6, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor

2 Images
Flow Cytometry - Anti-Fas antibody [EPR27359-51] (AB307591)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Fas antibody [EPR27359-51] (AB307591)

Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling Fas with ab307591 at 1/50 dilution (1µg) (Right panel) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left panel). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Cells were stained with rabbit IgG or ab307591, then stained with anti-CD45RO conjugated to BV421.

Flow Cytometry - Anti-Fas antibody [EPR27359-51] (AB307591)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Fas antibody [EPR27359-51] (AB307591)

Flow cytometric analysis of K562 (human chronic myelogenous leukemia lymphoblast) (Left panel) / Jurkat (human T cell leukemia T lymphocyte) (Right panel) cells labeling Fas with ab307591 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. Low expression : K-562 (PMID : 9168303).

関連する標識済み抗体及び組成の異なる製品 (4)

  • Carrier free

    Anti-Fas antibody [EPR27359-51] - BSA and Azide free

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Fas antibody [EPR27359-51]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Fas antibody [EPR27359-51]

  • 578 PE

    PE Anti-Fas antibody [EPR27359-51]

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR27359-51

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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組合せ製品

Assay Kits

AB183360

Human FAS ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human FAS ELISA Kit (AB183360)

  • 0
  • 0
Primary Antibodies

AB187091

Anti-MLKL (phospho S358) antibody [EPR9514]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-MLKL (phospho S358) antibody [EPR9514] (AB187091)

  • 4
  • 18
Primary Antibodies

AB184787

Anti-Caspase-3 antibody [EPR18297]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-3 antibody [EPR18297] (AB184787)

  • 3
  • 3
Primary Antibodies

AB316957

Anti-RIP3 antibody [EPR24374-9]

20ul selling size|Recombinant|RabMAb

Immunoprecipitation - Anti-RIP3 antibody [EPR24374-9] (AB316957)

  • 0
  • 0
Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Conditional Ambient
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase CASP8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs CASP8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
See full target information FAS
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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