AB216991

Anti-Fas 抗体 [EPR21088]

Anti-Fas antibody [EPR21088]

RabMAb
Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal Fas antibody. Suitable for IHC-Fr, Flow Cyt, ICC/IF and reacts with Mouse samples. Cited in 3 publications.

別名を表示する

CD95, Apt1, Tnfrsf6, Fas, Tumor necrosis factor receptor superfamily member 6, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor

4 Images

Immunohistochemistry (Frozen sections) - Anti-Fas antibody [EPR21088] (AB216991)

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Fas antibody [EPR21088] (AB216991)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse spleen tissue (fresh) labelling Fas with ab216991 at 1/100 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary at 1/1000 dilution. Positive staining on mouse spleen tissue is observed. The nuclear counterstain was DAPI (Blue).

Flow Cytometry - Anti-Fas antibody [EPR21088] (AB216991)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Fas antibody [EPR21088] (AB216991)

Flow cytometric analysis of primary mouse thymocytes labeling Fas with ab216991 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. Total viable cells were gated for the image.

Immunocytochemistry/ Immunofluorescence - Anti-Fas antibody [EPR21088] (AB216991)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Fas antibody [EPR21088] (AB216991)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized primary mouse thymocytes labeling Fas with ab216991 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on primary mouse thymocytes.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Negative control : L-929 (mouse connective tissue fibroblast cell line) (PMID : 7521365).

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Fas antibody [EPR21088] (AB216991)

Negative control : Flow cytometric analysis of L-929 (mouse connective tissue fibroblast cell line) cell line labeling Fas with ab216991 at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control : L-929 (PMID : 12077239).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR21088

アイソタイプ

IgG

キャリアフリー

交差種

Mouse

アプリケーション

ICC/IF, IHC-Fr, Flow Cyt

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Rat": { "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase CASP8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs CASP8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both (By similarity).

See full target information Fas

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Advanced healthcare materials 13:e2402973 PubMed39396375

2024

Targeting Hypoxia and Autophagy Inhibition via Delivering Sonodynamic Nanoparticles With HIF-2α Inhibitor for Enhancing Immunotherapy in Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yihao Zhu,Yajian Li,Xuwen Li,Yuan Yu,Lingpu Zhang,Hanchen Zhang,Can Chen,Dong Chen,Mingshuai Wang,Nianzeng Xing,Feiya Yang,Wahafu Wasilijiang,Xiongjun Ye

Cell death discovery 8:103 PubMed35256601

2022

LncRNA MEG3 up-regulates SIRT6 by ubiquitinating EZH2 and alleviates nonalcoholic fatty liver disease.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmei Zou,Liang Liu,Yinzhen Zeng,Huanhuan Wang,Dongling Dai,Mingguo Xu

Molecular medicine reports 22:5053-5068 PubMed33174043

2020

Identifying the mechanisms underlying the protective effect of tetramethylpyrazine against cisplatin‑induced in vitro ototoxicity in HEI‑OC1 auditory cells using gene expression profiling.

Applications

Unspecified application

Species

Unspecified reactive species

Guofang Guan,Xiao He,Jingjing Chen,Li Bin,Xuxia Tang

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Fas 抗体 [EPR21088]

Anti-Fas antibody [EPR21088]

Immunohistochemistry (Frozen sections) - Anti-Fas antibody [EPR21088] (AB216991)

Flow Cytometry - Anti-Fas antibody [EPR21088] (AB216991)

Immunocytochemistry/ Immunofluorescence - Anti-Fas antibody [EPR21088] (AB216991)

Flow Cytometry - Anti-Fas antibody [EPR21088] (AB216991)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

製品プロトコール

ターゲットの情報

文献 (3)

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組合せ製品

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Abcam product promise