Anti-Fas 抗体 [EOS9.1] - Low endotoxin, Azide free (ab185766)
![Flow Cytometry - Anti-Fas antibody [EOS9.1] - Low endotoxin, Azide free (ab185766)](https://www.abcam.co.jp/ps/products/185/ab185766/Images/ab185766-467404-anti-fas-antibody-flow-cytometry-jurkat-human.jpg "Flow Cytometry - Anti-Fas antibody [EOS9.1] - Low endotoxin, Azide free (ab185766)")
Key features and details
- Mouse monoclonal [EOS9.1] to Fas - Low endotoxin, Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgM
製品の概要
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製品名
Anti-Fas antibody [EOS9.1] - Low endotoxin, Azide free
Fas 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [EOS9.1] to Fas - Low endotoxin, Azide free -
由来種
Mouse -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
The details of the immunogen for this antibody are not available.
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ポジティブ･コントロール
- Human peripheral blood leukocytes; stimulated Jurkat cells.
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特記事項
Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by the LAL assay.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
aqueous buffer, no sodium azide -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
EOS9.1 -
アイソタイプ
IgM -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185766の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
1 µg/test |
| 特記事項 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. 1 µg/test |
ターゲット情報
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機能
Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). -
組織特異性
Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6. -
関連疾患
Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly. -
配列類似性
Contains 1 death domain.
Contains 3 TNFR-Cys repeats. -
ドメイン
Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 355 Human
- Omim: 134637 Human
- SwissProt: P25445 Human
- Unigene: 244139 Human
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別名
- ALPS 1A antibody
- ALPS1A antibody
- APO 1 antibody
see all
画像
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Flow Cytometry - Anti-Fas antibody [EOS9.1] - Low endotoxin, Azide free (ab185766)
Flow cytometric analysis of unstimulated Jurkat cells (left), or Jukar cells stimulated with 5 ug/mL ab185766 (right). The stimulated cells were stianed with Fixable Viability Dye eFluor® 780 and Annexin V Apoptosis Detection Kit eFluor® 450 then fixed and permeabilized with the Foxp3 Staining Buffer Set, and stained with Anti-Human PARP1 (Cleaved) PE. Total viable cells (Fixable Viability Dye eFluor® 780 negative) were used for analysis.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab185766 は論文での使用が確認できていません。
