Anti-FANCG 抗体 (ab5067)
Key features and details
- Rabbit polyclonal to FANCG
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FANCG antibody
FANCG 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FANCG -
由来種
Rabbit -
特異性
A BLAST analysis was used to suggest cross reactivity with FANCG protein from human and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
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アプリケーション
適用あり: WB, IHC-P, ELISAmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide:
MSRQTTSVGSSC
, corresponding to amino acids 1 - 12 of Human FANCG. -
ポジティブ･コントロール
- Recombinant Human FANCG protein (ab114925) can be used as a positive control in WB.
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特記事項
Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This affinity-purified antibody is directed against human FANCG protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5067の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/2000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
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| IHC-P |
1/500 - 1/2000.
|
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| ELISA |
1/10000 - 1/50000.
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| 特記事項 |
|---|
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WB
1/500 - 1/2000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). |
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IHC-P
1/500 - 1/2000. |
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ELISA
1/10000 - 1/50000. |
ターゲット情報
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機能
DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Candidate tumor suppressor gene. -
組織特異性
Highly expressed in testis and thymus. Found in lymphoblasts. -
関連疾患
Defects in FANCG are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair. -
配列類似性
Contains 4 TPR repeats. -
細胞内局在
Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic. - Information by UniProt
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参照データベース
- Entrez Gene: 2189 Human
- Omim: 602956 Human
- SwissProt: O15287 Human
- Unigene: 591084 Human
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別名
- DNA repair protein XRCC9 antibody
- FAG antibody
- FANCG antibody
see all
画像
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Western blot - Anti-FANCG antibody (ab5067)Anti-FANCG antibody (ab5067) at 1/500 dilution + HeLa whole cell lysate at 35 µg
Secondary
IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 69 kDa
Observed band size: 69 kDa
Additional bands at: 11 kDa, 24 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
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Datasheet download
参考文献 (5)
ab5067 は 5 報の論文で使用されています。
- Liu J et al. Activation of TLR7-mediated autophagy increases epileptic susceptibility via reduced KIF5A-dependent GABAA receptor transport in a murine model. Exp Mol Med 55:1159-1173 (2023). PubMed: 37258573
- Wang Y et al. Caffeic Acid Phenethyl Ester Suppresses Oxidative Stress and Regulates M1/M2 Microglia Polarization via Sirt6/Nrf2 Pathway to Mitigate Cognitive Impairment in Aged Mice following Anesthesia and Surgery. Antioxidants (Basel) 12:N/A (2023). PubMed: 36978961
- Gonsalvez DG et al. Imaging and Quantification of Myelin Integrity After Injury With Spectral Confocal Reflectance Microscopy. Front Mol Neurosci 12:275 (2019). PubMed: 31803018
- Song Z et al. STAT1 as a downstream mediator of ERK signaling contributes to bone cancer pain by regulating MHC II expression in spinal microglia. Brain Behav Immun 60:161-173 (2017). PubMed: 27742579
- Song ZP et al. Minocycline attenuates bone cancer pain in rats by inhibiting NF-?B in spinal astrocytes. Acta Pharmacol Sin 37:753-62 (2016). PubMed: 27157092
