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AB5065

Anti-FANCC 抗体

Anti-FANCC antibody

4

(6 Reviews)

|

(2 Publications)

Rabbit Polyclonal FANCC antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human FANCC aa 50-150.

別名を表示する

FAC, FACC, FANCC, Fanconi anemia group C protein, Protein FACC

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-FANCC antibody (AB5065)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FANCC antibody (AB5065)

ICC/IF image of ab5065 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5065, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Western blot - Anti-FANCC antibody (AB5065)
  • WB

Unknown

Western blot - Anti-FANCC antibody (AB5065)

Primary antibody incubated with PBS supplemented with 1% normal goat serum and 0.1% milk.

All lanes:

Western blot - Anti-FANCC antibody (ab5065) at 1/1500 dilution

All lanes:

HeLa whole cell lysate at 35 µg

Secondary

All lanes:

IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution

Predicted band size: 63 kDa

Observed band size: 33 kDa,60 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human FANCC aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Q00597

特異性

Detects a band at 60kDa in Hela cell lysate corresponding to FANCC.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
This affinity-purified antibody is directed against human FANCC protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
バッファー組成
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

FANCC also known as Fanconi Anemia Complementation Group C protein plays an important role in DNA repair. It has a molecular mass of approximately 63 kDa. Expressed in various tissues including bone marrow and blood FANCC interacts with other proteins to maintain genomic stability. The protein assists in correcting DNA interstrand crosslinks preventing damage that can lead to genetic disorders.
Biological function summary

The Fanconi Anemia Complementation Group C protein forms part of the Fanconi anemia (FA) protein complex. This complex participates in the repair of DNA damage and stabilization of the genome. Through its action FANCC cooperates with other FA proteins to activate the FA pathway which is essential for proper cellular response to DNA damage. By facilitating repair processes FANCC helps protect cells from mutation accumulation and chromosomal instability.

Pathways

Multiple processes integrate the role of FANCC within cellular repair mechanisms. The protein engages in the FA/BRCA pathway a critical route responsible for DNA damage repair. FANCC operates alongside proteins such as FANCD2 and BRCA2 within this pathway forming a coordinated response to preserve genomic integrity. It also participates in pathways that promote cell survival under genotoxic stress conditions.

FANCC is linked to two major health conditions. Mutations or dysfunctions in FANCC lead to Fanconi Anemia a rare genetic disorder characterized by bone marrow failure and cancer predisposition. Additionally FANCC also has associations with certain cancers where its role in DNA repair is compromised. Within these diseases proteins such as BRCA2 and other FA complex members act with FANCC highlighting its importance in maintaining cellular health and preventing disease development.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Upon IFNG induction, may facilitate STAT1 activation by recruiting STAT1 to IFNGR1.
See full target information FANCC
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cell cycle (Georgetown, Tex.) 10:3300-10 PubMed21926477

2011

The Fanconi anemia pathway is downregulated upon macrophage differentiation through two distinct mechanisms.

Applications

WB

Species

Human

Wei-Ting Lu,Kimon Lemonidis,Ross M Drayton,Thierry Nouspikel

Molecular cancer 9:127 PubMed20509860

2010

Genetic inactivation of the Fanconi anemia gene FANCC identified in the hepatocellular carcinoma cell line HuH-7 confers sensitivity towards DNA-interstrand crosslinking agents.

Applications

WB

Species

Human

Andreas Palagyi,Kornelia Neveling,Ursula Plinninger,Andreas Ziesch,Bianca-Sabrina Targosz,Gerald U Denk,Stephanie Ochs,Antonia Rizzani,Daniel Meier,Wolfgang E Thasler,Helmut Hanenberg,Enrico N De Toni,Florian Bassermann,Claus Schäfer,Burkhard Göke,Detlev Schindler,Eike Gallmeier
View all publications
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