AB5063

Anti-FANCA/FAA 抗体

Name: Anti-FANCA/FAA 抗体 (ab5063) | Abcam
Brand: Abcam Limited
SKU: AB5063
Price: 72000 JPY
Availability: InStock
Rating: 3 (3 reviews)

Anti-FANCA/FAA antibody

(3 Reviews)

(11 Publications)

Rabbit Polyclonal FANCA/FAA antibody. Suitable for WB, ELISA and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human FANCA aa 950 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

別名を表示する

FAA, FACA, FANCH, FANCA, Fanconi anemia group A protein, Protein FACA

1 Images

Unknown

Western blot - Anti-FANCA/FAA antibody (AB5063)

All lanes:

Western blot - Anti-FANCA/FAA antibody (ab5063) at 1/1500 dilution

All lanes:

HeLa whole cell lysate at 35 µg

Secondary

All lanes:

IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution

Predicted band size: 163 kDa

Observed band size: 130 kDa,42 kDa,75 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, ELISA

applications

免疫原

Synthetic Peptide within Human FANCA aa 950 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O15360

特異性

Detects a band at 130kDa in HeLa cell lysate that we believe corresponds to FANCA/FAA.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/15000 - 1/60000", "ELISA-species-notes": "<p></p>" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

This affinity-purified antibody is directed against human FANCA protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.

バッファー組成

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The FANCA protein also known as FAA protein is a critical component in the DNA repair mechanism. FANCA is approximately 162 kDa and shows widespread expression in the human body particularly in proliferating cells. This protein plays an essential role in maintaining genomic stability by participating in the repair of DNA interstrand crosslinks. FANCA's primary function involves its interaction with other proteins in the cell nucleus where DNA repair and replication processes occur.

Biological function summary

FANCA works as a part of the Fanconi anemia (FA) core complex involving around 13 proteins. This complex plays an important role in the FA DNA repair pathway facilitating the repair of interstrand DNA crosslinks. FANCA acts by orchestrating the recruitment and activation of downstream repair proteins which ensures the high-fidelity maintenance of the genetic material during cell division. Dysfunction in the FA complex where FANCA operates can lead to chromosomal instability and increased mutation rates.

Pathways

FANCA functions prominently in the Fanconi anemia pathway and is also involved in the homologous recombination repair pathway. In the FA pathway FANCA works closely with related proteins like FANCB and FANCC to initiate the DNA repair process. FANCA's interaction with these proteins allows it to execute its repair role effectively ensuring cellular genomic integrity. These pathways are critical for protecting cells from DNA damage during replication and shielding them from cancer development.

FANCA mutations are directly associated with Fanconi anemia a genetic disorder characterized by marrow failure cancer predisposition and various congenital abnormalities. Loss of FANCA function disrupts the repair pathway leading to cellular hypersensitivity to DNA-damaging agents. Additionally FANCA's impairment has been implicated in certain cancers like acute myeloid leukemia where its interaction with other FA proteins gets compromised leading to tumor progression.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.

See full target information FANCA

style

left-column

文献 (11)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35955545

2022

Correction of Fanconi Anemia Mutations Using Digital Genome Engineering.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher J Sipe,Mitchell G Kluesner,Samuel P Bingea,Walker S Lahr,Aneesha A Andrew,Minjing Wang,Anthony P DeFeo,Timothy L Hinkel,Kanut Laoharawee,John E Wagner,Margaret L MacMillan,Gregory M Vercellotti,Jakub Tolar,Mark J Osborn,R Scott McIvor,Beau R Webber,Branden S Moriarity

Cell stem cell 25:607-621.e7 PubMed31543367

2019

NHEJ-Mediated Repair of CRISPR-Cas9-Induced DNA Breaks Efficiently Corrects Mutations in HSPCs from Patients with Fanconi Anemia.

Applications

Unspecified application

Species

Unspecified reactive species

Francisco José Román-Rodríguez,Laura Ugalde,Lara Álvarez,Begoña Díez,María José Ramírez,Cristina Risueño,Marta Cortón,Massimo Bogliolo,Sara Bernal,Francesca March,Carmen Ayuso,Helmut Hanenberg,Julián Sevilla,Sandra Rodríguez-Perales,Raúl Torres-Ruiz,Jordi Surrallés,Juan Antonio Bueren,Paula Río

Experimental hematology 43:1031-1046.e12 PubMed26366677

2015

FANCA safeguards interphase and mitosis during hematopoiesis in vivo.

Applications

Species

Unspecified reactive species

Zahi Abdul-Sater,Donna Cerabona,Elizabeth Sierra Potchanant,Zejin Sun,Rikki Enzor,Ying He,Kent Robertson,W Scott Goebel,Grzegorz Nalepa

Stem cell research 13:240-50 PubMed25108529

2014

FANCA knockout in human embryonic stem cells causes a severe growth disadvantage.

Applications

Species

Unspecified reactive species

Kim Vanuytsel,Qing Cai,Nisha Nair,Satish Khurana,Swati Shetty,Joris R Vermeesch,Laura Ordovas,Catherine M Verfaillie

EMBO molecular medicine 6:835-48 PubMed24859981

2014

Targeted gene therapy and cell reprogramming in Fanconi anemia.

Applications

Species

Human

Paula Rio,Rocio Baños,Angelo Lombardo,Oscar Quintana-Bustamante,Lara Alvarez,Zita Garate,Pietro Genovese,Elena Almarza,Antonio Valeri,Begoña Díez,Susana Navarro,Yaima Torres,Juan P Trujillo,Rodolfo Murillas,Jose C Segovia,Enrique Samper,Jordi Surralles,Philip D Gregory,Michael C Holmes,Luigi Naldini,Juan A Bueren

Cancer research 73:3393-401 PubMed23633493

2013

Regulation of FANCD2 by the mTOR pathway contributes to the resistance of cancer cells to DNA double-strand breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Changxian Shen,Duane Oswald,Doris Phelps,Hakan Cam,Christopher E Pelloski,Qishen Pang,Peter J Houghton

Cell cycle (Georgetown, Tex.) 10:3300-10 PubMed21926477

2011

The Fanconi anemia pathway is downregulated upon macrophage differentiation through two distinct mechanisms.

Applications

Species

Human

Wei-Ting Lu,Kimon Lemonidis,Ross M Drayton,Thierry Nouspikel

PloS one 6:e22911 PubMed21826217

2011

Fanconi anemia core complex gene promoters harbor conserved transcription regulatory elements.

Applications

Species

Unspecified reactive species

Daniel Meier,Detlev Schindler

PLoS genetics 6:e1000983 PubMed20548951

2010

The E1A-associated p400 protein modulates cell fate decisions by the regulation of ROS homeostasis.

Applications

Species

Human

Lise Mattera,Céline Courilleau,Gaëlle Legube,Takeshi Ueda,Rikiro Fukunaga,Martine Chevillard-Briet,Yvan Canitrot,Fabrice Escaffit,Didier Trouche

Blood 115:3453-62 PubMed20089964

2010

Knockdown of Fanconi anemia genes in human embryonic stem cells reveals early developmental defects in the hematopoietic lineage.

Applications

Species

Human

Asmin Tulpule,M William Lensch,Justine D Miller,Karyn Austin,Alan D'Andrea,Thorsten M Schlaeger,Akiko Shimamura,George Q Daley

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FANCA/FAA 抗体

Anti-FANCA/FAA antibody

Western blot - Anti-FANCA/FAA antibody (AB5063)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (11)

Abcam product promise