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リコンビナントRabMAb

Anti-FAM50A/XAP5 抗体 [EPR12816] (ab186410)

Reviews (1) Submit a question References (1)
Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)
  • Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)
  • Immunocytochemistry/ Immunofluorescence - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)
  • Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12816] to FAM50A/XAP5
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-FAM50A/XAP5 antibody [EPR12816]
    FAM50A/XAP5 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR12816] to FAM50A/XAP5

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Jurkat, Hela 293, HepG2, C6, Raw264.7, PC-12, NIH-3T3 cell lysates, human colon tissue, Hela cells

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR12816

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab186410の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/500.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/500.

ターゲット情報

  • 機能

    May be a DNA-binding protein or transcriptional factor.

  • 組織特異性

    Expressed in all tissues examined. Mostly abundant in fetal brain, liver and kidney; in the adult, high levels were also observed in heart, skeletal muscle, spleen, thymus, prostate and small intestine.

  • 配列類似性

    Belongs to the FAM50 family.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 9F antibody
      • DXS9928E antibody
      • FA50A_HUMAN antibody
      • FAM 50A antibody
      • fam50a antibody
      • Family with sequence similarity 50 member A antibody
      • HXC 26 antibody
      • HXC26 antibody
      • OTTHUMP00000061460 antibody
      • Protein FAM50A antibody
      • Protein HXC 26 antibody
      • Protein HXC-26 antibody
      • Protein HXC26 antibody
      • Protein XAP 5 antibody
      • Protein XAP-5 antibody
      • Protein XAP5 antibody
      • XAP 5 antibody
      • XAP 5 protein antibody
      • XAP5 antibody
      • XAP5 gene antibody
      • XAP5 protein antibody
      see all

    画像

    • Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)
      Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)
      All lanes : Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Hela cell lysate
      Lane 3 : 293 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 40 kDa

    • Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)
      Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)
      All lanes : Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410) at 1/1000 dilution

      Lane 1 : C6 cell lysate
      Lane 2 : RAW 264.7 cell lysate
      Lane 3 : PC-12 cell lysate
      Lane 4 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 40 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling FAM50A/XAP5 with ab186410 at 1/500 and ready to use HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)
      Immunocytochemistry/ Immunofluorescence - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)

      Immunocytochemical analysis of Hela cells fixed in 4% paraformaldehyde labling FAM50A/XAP5 with ab186410 at 1/500 dilution and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 dilution.

    • Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)
      Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)

    参考文献 (1)

    ab186410 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab186410 は 1 報の論文で使用されています。

    • Thompson NA  et al. Combinatorial CRISPR screen identifies fitness effects of gene paralogues. Nat Commun 12:1302 (2021). PubMed: 33637726

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Western blot abreview for Anti-FAM50A antibody [EPR12816]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Melanoma cells)
    Gel Running Conditions
    Non-reduced Denaturing
    Loading amount
    10 µg
    Treatment
    3 cell lysates with FAM50A frameshift mutation removing the protein, 2 cell lysates with FAM50A wild type
    Specification
    Melanoma cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Nicola Thompson

    Verified customer

    投稿 Jan 23 2018

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