Anti-FAM222B 抗体 (ab151147)
Key features and details
- Rabbit polyclonal to FAM222B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FAM222B antibody -
製品の詳細
Rabbit polyclonal to FAM222B -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to amino acids 111-189 of Human FAM222B (UniProt Q8WU58)
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ポジティブ･コントロール
- Human rectum tissue. RT-4, U-251 MG, Human Liver and Human Tonsil lysates.
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特記事項
The antibody solution should be gently mixed before use.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab151147の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 60 kDa.
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 60 kDa. |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
ターゲット情報
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関連性
Belongs to the FAM222 family. -
参照データベース
- Entrez Gene: 55731 Human
- SwissProt: Q8WU58 Human
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別名
- C17orf63 antibody
- family with sequence similarity 222, member B antibody
- protein FAM222B antibody
- uncharacterized protein C17orf63 antibody
画像
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Western blot - Anti-FAM222B antibody (ab151147)All lanes : Anti-FAM222B antibody (ab151147) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human Tonsil lysate
Predicted band size: 60 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM222B antibody (ab151147)Immunohistochemical analysis of paraffin embedded Human rectum tissue labelling FAM222B with ab151147 antibody at a dilution of 1/50. -
Immunocytochemistry/ Immunofluorescence - Anti-FAM222B antibody (ab151147)Immunofluorescent staining of human cell line CACO-2 shows positivity in nucleus but excluded from the nucleoli.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab151147 は論文での使用が確認できていません。