Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-FAM111A 抗体 [EPR14407] (ab184572)

Submit a review Submit a question References (6)
Western blot - Anti-FAM111A antibody [EPR14407] (ab184572)
  • Immunocytochemistry/ Immunofluorescence - Anti-FAM111A antibody [EPR14407] (ab184572)
  • Anti-FAM111A antibody [EPR14407] (ab184572)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14407] to FAM111A
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-FAM111A antibody [EPR14407]
    FAM111A 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14407] to FAM111A

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, ICC/IFmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • SW480, BxPC-3 or T47-D cell lysates; T47-D cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR14407

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184572の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
ICC/IF
1/100.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).
ICC/IF
1/100.

ターゲット情報

  • 関連性

    This protein of unknown function belongs to the FAM111 family.

  • 参照データベース

    • 別名

      • Family with sequence similarity 111 member A antibody
      • GCLEB antibody
      • Hypothetical protein LOC63901 antibody
      • KCS2 antibody
      • KIAA1895 antibody
      • Protein FAM111A antibody
      • RGD1560913 antibody
      see all

    画像

    • Western blot - Anti-FAM111A antibody [EPR14407] (ab184572)
      Western blot - Anti-FAM111A antibody [EPR14407] (ab184572)
      All lanes : Anti-FAM111A antibody [EPR14407] (ab184572) at 1/1000 dilution

      Lane 1 : SW480 cell lysate
      Lane 2 : BxPC-3 cell lysate
      Lane 3 : T47-D cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 70 kDa
      Observed band size: 70 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-FAM111A antibody [EPR14407] (ab184572)
      Immunocytochemistry/ Immunofluorescence - Anti-FAM111A antibody [EPR14407] (ab184572)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed T47-D cells labeling FAM111A with ab184572 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/1200 dilution (red). Counter stained with Dapi (blue).

    • Anti-FAM111A antibody [EPR14407] (ab184572)
      Anti-FAM111A antibody [EPR14407] (ab184572)

    参考文献 (6)

    ab184572 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab184572 は 6 報の論文で使用されています。

    • Gan K  et al. Positive feedback loop of lncRNA FAM201A/miR-146a-5p/POU2F1 regulates IL-1ß-induced chondrocyte injury in vitro. Mol Med Rep 25:N/A (2022). PubMed: 34796909
    • Nie M  et al. FAM111A induces nuclear dysfunction in disease and viral restriction. EMBO Rep 22:e50803 (2021). PubMed: 33369867
    • Kojima Y  et al. FAM111A protects replication forks from protein obstacles via its trypsin-like domain. Nat Commun 11:1318 (2020). PubMed: 32165630
    • Hoffmann S  et al. FAM111 protease activity undermines cellular fitness and is amplified by gain-of-function mutations in human disease. EMBO Rep 21:e50662 (2020). PubMed: 32776417
    • Tarnita RM  et al. Contribution of DNA Replication to the FAM111A-Mediated Simian Virus 40 Host Range Phenotype. J Virol 93:N/A (2019). PubMed: 30333173
    • Panda D  et al. Triad of human cellular proteins, IRF2, FAM111A, and RFC3, restrict replication of orthopoxvirus SPI-1 host-range mutants. Proc Natl Acad Sci U S A 114:3720-3725 (2017). PubMed: 28320935

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab184572.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com