Anti-FAK (phospho Y576 + Y577) 抗体 [EP1832Y] (ab76244)
![Dot Blot - Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244)](https://www.abcam.co.jp/ps/products/76/ab76244/Images/ab76244-281384-anti-fak-phospho-y576-y577-antibody-ep1832y-dot-blot.jpg "Dot Blot - Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1832Y] to FAK (phospho Y576 + Y577)
- Suitable for: WB, Dot blot
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y]
FAK 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1832Y] to FAK (phospho Y576 + Y577) -
由来種
Rabbit -
アプリケーション
適用あり: WB, Dot blotmore details
適用なし: IHC-P -
種交差性
交差種: Mouse
交差が予測される動物種: Human
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- NIH-3T3 lysate treated with pervanadate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1832Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76244の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/50000 - 1/100000. Predicted molecular weight: 119 kDa.
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| Dot blot |
1/1000.
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| 特記事項 |
|---|
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WB
1/50000 - 1/100000. Predicted molecular weight: 119 kDa. |
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Dot blot
1/1000. |
追加情報
Is unsuitable for IHC-P.
ターゲット情報
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機能
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity. -
組織特異性
Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain. -
ドメイン
The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions. -
翻訳後修飾
Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1. -
細胞内局在
Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions. - Information by UniProt
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参照データベース
- Entrez Gene: 5747 Human
- Entrez Gene: 14083 Mouse
- Omim: 600758 Human
- SwissProt: Q05397 Human
- SwissProt: P34152 Mouse
- Unigene: 395482 Human
- Unigene: 254494 Mouse
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別名
- FADK 1 antibody
- FADK antibody
- FAK related non kinase polypeptide antibody
see all
画像
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Dot Blot - Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244)
Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244) Dot Blot. Primary ab dilution 1:1000, Secondary ab description and code (ab id), Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), Secondary ab dilution 1:100,000, Blocking buffer and concentration 5% NFDM/TBST, Diluting buffer and concentration 5% NFDM /TBST, Lane 1:FAK (pY576+pY577) phospho peptide, Lane 2:FAK (pY576) phospho peptide, Lane 3:FAK (pY577) phospho peptide, Lane 4:FAK non-phospho peptide, Exposure time 3 minutes. Note: antibody used for ab76244 is purified batch.
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![Western blot - Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244)](https://www.abcam.co.jp/ps/products/76/ab76244/Images/ab76244-91608-anti-fak-phospho-y576-y577-antibody-ep1832y-western-blot.jpg)
Western blot - Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244)All lanes : Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244) at 1/50000 dilution
Lane 1 : NIH-3T3 cell lysates, untreated
Lane 2 : NIH-3T3 cell lysates treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/1000 dilution
Predicted band size: 119 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted? -
![Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244)](https://www.abcam.co.jp/ps/products/76/ab76244/Images/ab76244-3-benefits-of-recombinant-antibodies.png)
Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (ab76244)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab76244 は 13 報の論文で使用されています。
- Kadir ND et al. Electrospun fibers enhanced the paracrine signaling of mesenchymal stem cells for cartilage regeneration. Stem Cell Res Ther 12:100 (2021). PubMed: 33536060
- Jiang K et al. Exosome-derived ENO1 regulates integrin a6ß4 expression and promotes hepatocellular carcinoma growth and metastasis. Cell Death Dis 11:972 (2020). PubMed: 33184263
- Cho HJ et al. Identification of SYK inhibitor, R406 as a novel senolytic agent. Aging (Albany NY) 12:8221-8240 (2020). PubMed: 32379705
- Tsukamoto T et al. Src mediates TGF-β-induced intraocular pressure elevation in glaucoma. J Cell Physiol 234:1730-1744 (2019). PubMed: 30144071
- Husari A et al. On the relationship of YAP and FAK in hMSCs and osteosarcoma cells: Discrimination of FAK modulation by nuclear YAP depletion or YAP silencing. Cell Signal 63:109382 (2019). PubMed: 31376525