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AB81298

Anti-FAK (phospho Y397) 抗体 [EP2160Y]

Anti-FAK (phospho Y397) antibody [EP2160Y]

4

(2 Reviews)

|

(139 Publications)

Anti-FAK (phospho Y397) antibody [EP2160Y] (ab81298) is a rabbit monoclonal antibody detecting FAK in Western Blot, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 130 publications
- Trusted since 2009

別名を表示する

FAK, FAK1, PTK2, Focal adhesion kinase 1, FADK 1, Focal adhesion kinase-related nonkinase, Protein phosphatase 1 regulatory subunit 71, Protein-tyrosine kinase 2, p125FAK, pp125FAK, FRNK, PPP1R71

4 Images
Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (AB81298)
  • WB

Unknown

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (AB81298)

All lanes:

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (ab81298) at 1/2000 dilution

Lane 1:

human brain tissue lysates, untreated at 10 µg

Lane 2:

human brain tissue lysates treated with AP at 10 µg

Lane 3:

rat brain tissue lysates, untreated at 10 µg

Lane 4:

rat brain tissue lysates treated with AP at 10 µg

Secondary

All lanes:

HRP labelled goat anti rabbit at 1/2000 dilution

Predicted band size: 119 kDa

Observed band size: 119 kDa

false

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (AB81298)
  • WB

Supplier Data

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (AB81298)

Blocking buffer and concentration :  5% NFDM/TBST.  Diluting buffer and concentration :  5% NFDM/TBST. The activation of phosphorylation of FAK is reported to be related to developmental processes in brain tissue (PMID : 14642275, PMID : 21118706). So expression level of phosphorylated modified FAK in normal brain is quite low causing not easy to be detected. We suggest optimizing experimental protocols (increasing lysate amount, using lower dilution or higher sensitivity ECL substrate) to improve results. ab181602 has been used as a loading control.

Lane 1:

Untreated NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Lanes 1 - 5:

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (ab81298) at 1/1000 dilution

Lane 2:

NIH/3T3 treated with 10mM Pervanadate for 60 min whole cell lysate at 20 µg

Lane 3:

Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

HeLa treated with 10mM Pervanadate for 60 min whole cell lysate at 20 µg

Lane 5:

Mouse brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 119 kDa

Observed band size: 119 kDa

false

Exposure time: 40s

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (AB81298)
  • WB

Unknown

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (AB81298)

Blocking Buffer : 5% NFDM/TBST

Dilution Buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (ab81298) at 1/2000 dilution

Lane 1:

Untreated mouse brain at 10 µg

Lane 2:

Mouse brain treated with alkaline phosphatase at 10 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 119 kDa

Observed band size: 119 kDa

false

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (AB81298)
  • WB

Unknown

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (AB81298)

Blocking Buffer : 5% NFDM/TBST

Dilution Buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (ab81298) at 1/1000 dilution

Lane 1:

Untreated rat brain at 10 µg

Lane 2:

Rat brain treated with alkaline phosphatase at 10 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit IgG (H+L) at 1000 µg

Predicted band size: 119 kDa

Observed band size: 119 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-FAK (phospho Y397) antibody [EP2160Y] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP2160Y

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

The activation of phosphorylation of FAK is reported to be related to developmental processes in brain tissue (PMID: 14642275, PMID: 21118706). So expression level of phosphorylated modified FAK in normal brain is quite low causing not easy to be detected. We suggest optimizing experimental protocols (increasing lysate amount, using lower dilution or higher sensitivity ECL substrate) to improve results.

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Reactivity data

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下記製品にもご興味をお持ちいただけるかもしれません:

AB279795

Phospho-FAK (Y397) ELISA Kit

0

0 Reviews

製品ページを見る
こちらの製品も推奨する理由

この製品は、同様の実験や関連する研究で広く使用されていることから推奨させていただいております。

お客様の実験に適するかご確認いただくため、必ず製品データシートをご確認いただくことをお勧めいたします。ご不明点等ございましたら、テクニカルサポートチームまでお問い合わせください。

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製品の詳細

What is this antibody validated in?
Anti-FAK (phospho Y397) antibody [EP2160Y] (ab81298) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of FAK?
Anti-FAK (phospho Y397) [EP2160Y] (ab81298) specifically detects a band for FAK (UniProt: Q05397) at a molecular weight of 119kDa.

Trusted by the scientific community
Anti-FAK (phospho Y397) [EP2160Y] (ab81298) was first used in a scientific publication in 2009 and has been cited over 130 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EP2160Y] also available for your convenience: ab81298, Carrier free - ab223529, Carrier free - ab271862

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Focal Adhesion Kinase (FAK) also known as Protein Tyrosine Kinase 2 (PTK2) is a non-receptor tyrosine kinase. This protein has a molecular weight of approximately 125 kDa. FAK is expressed at high levels in brain muscle and liver tissues. Mechanically FAK plays a role in cellular adhesion and migration by regulating integrin signaling and cell-extracellular matrix interactions. FAK auto-phosphorylates at tyrosine residue 397 creating a binding site for Src family kinases and promoting downstream signaling pathways.
Biological function summary

Focal Adhesion Kinase participates in the formation of focal adhesions which are complexes that connect the cytoskeleton to the extracellular matrix. The FAK protein functions as an important signaling node in these structures allowing for the assembly of multiprotein signal transduction complexes. FAK also controls cellular processes such as spreading motility and survival. The interaction with proteins such as Src kinases paxillin and talin facilitates its biological roles in cell signaling.

Pathways

Focal Adhesion Kinase engages in the regulation of the MAPK/ERK signaling pathway and the PI3K/AKT pathway. These pathways are instrumental for cell proliferation survival and migration. In these pathways FAK interacts with proteins such as PI3K Grb2 and Sos linking integrin-mediated signals with downstream effects that influence cell behavior and survival.

Altered FAK signaling has ties to cancer progression and metastasis as well as cardiovascular diseases. In cancer the overexpression of FAK and its interaction with proteins like Src and VEGFR can drive tumor growth and angiogenesis. In cardiovascular diseases improper FAK activation can lead to aberrant heart tissue remodeling and associated pathologies. Abnormalities in FAK signaling pathways can therefore contribute significantly to the development and progression of these diseases.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Phosphorylates NEDD9 following integrin stimulation (PubMed : 9360983). Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.. Isoform 6. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.
See full target information PTK2 phospho Y397
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文献 (139)

Recent publications for all applications. Explore the full list and refine your search

International journal of medical sciences 21:137-150 PubMed38164350

2024

Enhanced Bone Regeneration through Regulation of Mechanoresponsive FAK-ERK1/2 Signaling by ZINC40099027 during Distraction Osteogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Shanyu Li,Hongxiao Wu,Feng Wang,Lingchi Kong,Yifan Yu,Rongtai Zuo,Haoyu Zhao,Jia Xu,Qinglin Kang

Biological research 56:66 PubMed38057829

2023

The novel roles of YULINK in the migration, proliferation and glycolysis of pulmonary arterial smooth muscle cells: implications for pulmonary arterial hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Chia Wu,Wei-Ting Wang,Ming-Chun Yang,Yu-Tsun Su,Jwu-Lai Yeh,Jong-Hau Hsu,Jiunn-Ren Wu

Communications biology 6:1137 PubMed37945735

2023

Increasing pulse pressure ex vivo, mimicking acute physical exercise, induces smooth muscle cell-mediated de-stiffening of murine aortic segments.

Applications

Unspecified application

Species

Unspecified reactive species

Cédric H G Neutel,Anne-Sophie Weyns,Arthur Leloup,Sofie De Moudt,Pieter-Jan Guns,Paul Fransen

Journal of inflammation research 16:5095-5109 PubMed38026263

2023

LPA Alleviates Septic Acute Lung Injury via Protective Endothelial Barrier Function Through Activation of PLC-PKC-FAK.

Applications

Unspecified application

Species

Unspecified reactive species

Ruifeng Bai,Jianqiu Pei,Shengqiang Pei,Xiangfeng Cong,Jerold Chun,Fang Wang,Xi Chen

Frontiers in immunology 14:1167605 PubMed38022556

2023

Semaphorin 7a aggravates TGF-β1-induced airway EMT through the FAK/ERK1/2 signaling pathway in asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Haiying Peng,Fei Sun,Yunxiu Jiang,Zihan Guo,Xinyi Liu,Anli Zuo,Degan Lu

Journal of materials science. Materials in medicine 34:54 PubMed37884819

2023

Unusual effects of a nanoporous gold substrate on cell adhesion and differentiation because of independent multi-branch signaling of focal adhesions.

Applications

Unspecified application

Species

Unspecified reactive species

Peizheng Wu,Kazuya Yanagi,Kazuki Yokota,Masataka Hakamada,Mamoru Mabuchi

EMBO reports 24:e56850 PubMed37846507

2023

An ULK1/2-PXN mechanotransduction pathway suppresses breast cancer cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Peigang Liang,Jiaqi Zhang,Yuchen Wu,Shanyuan Zheng,Zhaopeng Xu,Shuo Yang,Jinfang Wang,Suibin Ma,Li Xiao,Tianhui Hu,Wenxue Jiang,Chaoqun Huang,Qiong Xing,Mondira Kundu,Bo Wang

Journal of biomedical materials research. Part A 112:65-81 PubMed37723658

2023

Heparin-collagen I bilayers stimulate FAK/ERK½ signaling via α2β1 integrin to support the growth and anti-inflammatory potency of mesenchymal stromal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Said J Cifuentes,Maribella Domenech

Frontiers in physiology 14:1218924 PubMed37637147

2023

The effect of cyclic stretch on aortic viscoelasticity and the putative role of smooth muscle focal adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Cédric H G Neutel,Callan D Wesley,Guido R Y De Meyer,Wim Martinet,Pieter-Jan Guns

Journal of translational medicine 21:522 PubMed37533102

2023

Downregulation of mitochondrial complex I induces ROS production in colorectal cancer subtypes that differently controls migration.

Applications

Unspecified application

Species

Unspecified reactive species

Jean Bastin,Marine Sroussi,Ivan Nemazanyy,Pierre Laurent-Puig,Sophie Mouillet-Richard,Fatima Djouadi
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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