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リコンビナントRabMAb

Anti-FADD (phospho S194) 抗体 [EPR1816Y] (ab68475)

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Western blot - Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)
  • Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1816Y] to FADD (phospho S194)
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-FADD (phospho S194) antibody [EPR1816Y]
    FADD 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR1816Y] to FADD (phospho S194)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF or IHC-P

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human FADD aa 150-250 (phospho S194). The exact sequence is proprietary.

  • ポジティブ･コントロール

    • Jurkat cell lysate

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab68475の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
特記事項
追加情報
IP: 1/30.
WB: 1/500. Detects a band of approximately 27 kDa (predicted molecular weight: 23 kDa).

Is unsuitable for Flow Cyt, ICC/IF or IHC-P.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

ターゲット情報

  • 機能

    Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.

  • 組織特異性

    Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.

  • 配列類似性

    Contains 1 death domain.
    Contains 1 DED (death effector) domain.

  • ドメイン

    Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
  • Information by UniProt

  • 参照データベース

    • 別名

      • FADD antibody
      • FADD protein antibody
      • FADD_HUMAN antibody
      • Fas (TNFRSF6) associated via death domain antibody
      • Fas associated via death domain antibody
      • Fas associating death domain containing protein antibody
      • Fas associating protein antibody
      • Fas associating protein with death domain antibody
      • Fas TNFRSF6 associated via death domain antibody
      • FAS-associated death domain protein antibody
      • FAS-associating death domain-containing protein antibody
      • GIG 3 antibody
      • GIG3 antibody
      • Growth inhibiting gene 3 protein antibody
      • Growth-inhibiting gene 3 protein antibody
      • H sapiens mRNA for mediator of receptor induced toxicity antibody
      • Mediator of receptor induced toxicity antibody
      • MGC8528 antibody
      • MORT 1 antibody
      • MORT1 antibody
      • Protein FADD antibody
      see all

    画像

    • Western blot - Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)
      Western blot - Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)
      All lanes : Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475) at 1/500 dilution

      Lane 1 : Un-treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
      Lane 2 : Calyculin A treated Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP conjugated Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 23 kDa
      Observed band size: 27 kDa why is the actual band size different from the predicted?

    • Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)
      Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)

    参考文献 (0)

    ab68475 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab68475 は論文での使用が確認できていません。

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