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AB108601

Anti-FADD 抗体 [EPR4415]

Anti-FADD antibody [EPR4415]

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(10 Publications)

Rabbit Recombinant Monoclonal FADD antibody. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 10 publications.

別名を表示する

MORT1, GIG3, FADD, FAS-associated death domain protein, FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, Mediator of receptor induced toxicity

6 Images
Western blot - Anti-FADD antibody [EPR4415] (AB108601)
  • WB

Lab

Western blot - Anti-FADD antibody [EPR4415] (AB108601)

Lanes 1 - 2 : Merged signal (red and green). Green - ab108601 observed at 23 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab108601 was shown to react with FADD in wild-type HeLa cells in western blot with loss of signal observed in FADD knockout cell line ab261817 (FADD knockout cell lysate ab257261). Wild-type and FADD knockout HeLa cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab108601 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-FADD antibody [EPR4415] (ab108601) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

FADD knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human FADD knockout HeLa cell line (ab261817)

Predicted band size: 23 kDa

Observed band size: 23 kDa

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Western blot - Anti-FADD antibody [EPR4415] (AB108601)
  • WB

Unknown

Western blot - Anti-FADD antibody [EPR4415] (AB108601)

All lanes:

Western blot - Anti-FADD antibody [EPR4415] (ab108601) at 1/1000 dilution

Lane 1:

A431 cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

SKBR-3 cell lysate at 10 µg

Predicted band size: 23 kDa

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Flow Cytometry (Intracellular) - Anti-FADD antibody [EPR4415] (AB108601)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-FADD antibody [EPR4415] (AB108601)

Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling FADD with purified ab108601 at 1/140 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody [EPR4415] (AB108601)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody [EPR4415] (AB108601)

Immunohistochemical staining of paraffin-embedded Human kidney tissue using ab108601 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunoprecipitation - Anti-FADD antibody [EPR4415] (AB108601)
  • IP

Lab

Immunoprecipitation - Anti-FADD antibody [EPR4415] (AB108601)

FADD was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg with 108601 at 1/120 dilution (2μg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg

Lane 2 : ab108601 IP in HeLa whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab108601 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-FADD antibody [EPR4415] (ab108601)

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

Western blot - Anti-FADD antibody [EPR4415] (AB108601)
  • WB

Lab

Western blot - Anti-FADD antibody [EPR4415] (AB108601)

Lanes 1 - 4 : Merged signal (red and green). Green - ab108601 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab108601 was shown to specifically react with FADD when FADD knockout samples were used. Wild-type and FADD knockout samples were subjected to SDS-PAGE. ab108601 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-FADD antibody [EPR4415] (ab108601) at 1/1000 dilution

Lane 1:

Wild-type HAP1 cell lysate at 20 µg

Lane 2:

FADD knockout HAP1 cell lysate at 20 µg

Lane 3:

A431 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Predicted band size: 23 kDa

false

関連する標識済み抗体及び組成の異なる製品 (2)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR4415

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, IP, Flow Cyt (Intra), IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製度
Tissue culture supernatant
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Stable for 12 months at -20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

FADD also known as Fas-Associated protein with Death Domain is an adaptor molecule with a molecular weight of approximately 23 kDa. It plays a critical role in the transmission of apoptotic signals. FADD is widely expressed in various tissues particularly in the thymus and immune system cells. This protein serves as a bridge linking death receptors like Fas and TNFR-1 with caspase activation pathways.
Biological function summary

FADD is essential in apoptosis where it assists in the assembly of the death-inducing signaling complex (DISC). Upon receptor activation FADD recruits procaspase-8 or -10 to DISC promoting their autocatalytic cleavage and activation. This leads to the subsequent cascade that results in cell apoptosis. FADD also plays a role in necroptosis and is involved in the immune response regulation highlighting its multifunctional nature in cellular processes.

Pathways

FADD integrates into the apoptotic and necroptotic pathways. In the apoptotic pathway it interacts closely with Fas a death receptor to promote caspase-8 activation. Additionally in the necroptotic pathway FADD associates with RIP1 and RIP3 contributing to an alternative form of programmed cell death. These interactions underline its significant role in controlling cell fate decisions.

Aberrations in FADD function are associated with cancer and autoimmune diseases. Overexpression or mutation of FADD can lead to unchecked cell proliferation or defective apoptosis contributing to cancer development. In autoimmune disorders improper regulation of FADD may disrupt immune tolerance and lead to systemic inflammation. Key proteins involved in these disease processes include caspase-8 and RIPK1 which interact with FADD in regulating cell death and survival mechanisms.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Apoptotic adapter molecule that recruits caspases CASP8 or CASP10 to the activated FAS/CD95 or TNFRSF1A/TNFR-1 receptors (PubMed : 16762833, PubMed : 19118384, PubMed : 20935634, PubMed : 23955153, PubMed : 24025841, PubMed : 7538907, PubMed : 9184224). The resulting aggregate called the death-inducing signaling complex (DISC) performs CASP8 proteolytic activation (PubMed : 16762833, PubMed : 19118384, PubMed : 20935634, PubMed : 7538907, PubMed : 9184224). Active CASP8 initiates the subsequent cascade of caspases mediating apoptosis (PubMed : 16762833). Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling (PubMed : 21109225).
See full target information FADD
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文献 (10)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastroenterology 29:1875-1898 PubMed37032730

2023

Antihepatoma peptide, scolopentide, derived from the centipede scolopendra subspinipes mutilans.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Xing Hu,Zhuo Liu,Zhen Zhang,Zhe Deng,Zhen Huang,Ting Feng,Qing-Hong Zhou,Si Mei,Chun Yi,Qing Zhou,Pu-Hua Zeng,Gang Pei,Sha Tian,Xue-Fei Tian

International journal of general medicine 15:4037-4052 PubMed35444456

2022

Clinical Impact of 11q13.3 Amplification on Immune Cell Infiltration and Prognosis in Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Renyu Zhou,Xiaoxi Zhu,Yulong Peng,Lijuan Zhong,Lilin Peng,Bo Yang,Yuhua Meng,Xuanzhao Chen,Yuanzhi Lu

Signal transduction and targeted therapy 6:375 PubMed34728602

2021

The existence of a nonclassical TCA cycle in the nucleus that wires the metabolic-epigenetic circuitry.

Applications

Unspecified application

Species

Unspecified reactive species

Xujun Liu,Wenzhe Si,Lin He,Jianguo Yang,Yani Peng,Jie Ren,Xiaoping Liu,Tong Jin,Huajing Yu,Zihan Zhang,Xiao Cheng,Wenting Zhang,Lu Xia,Yunchao Huang,Yue Wang,Shumeng Liu,Lin Shan,Yu Zhang,Xiaohan Yang,Haixia Li,Jing Liang,Luyang Sun,Yongfeng Shang

Cell death discovery 7:33 PubMed33597510

2021

Heparanase induces necroptosis of microvascular endothelial cells to promote the metastasis of hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaopeng Chen,Bin Cheng,Dafei Dai,Yuhai Wu,Zhiwen Feng,Chaogang Tong,Xiangming Wang,Jun Zhao

BMC cancer 19:831 PubMed31443698

2019

Prognostic value of biomarkers EpCAM and αB-crystallin associated with lymphatic metastasis in breast cancer by iTRAQ analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Zeng,Xiyun Deng,Jingmin Zhong,Li Yuan,Xiaojun Tao,Sai Zhang,Yong Zeng,Guangchun He,Pingping Tan,Yongguang Tao

The Journal of biological chemistry 294:991-1004 PubMed30504227

2018

Herpes simplex virus 1 ICP6 impedes TNF receptor 1-induced necrosome assembly during compartmentalization to detergent-resistant membrane vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Ali,Linda Roback,Edward S Mocarski

Cell death & disease 9:346 PubMed29497034

2018

Proteasome inhibition blocks necroptosis by attenuating death complex aggregation.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Ali,Edward S Mocarski

Oncology reports 37:2839-2846 PubMed28393201

2017

SPAG6 regulates cell apoptosis through the TRAIL signal pathway in myelodysplastic syndromes.

Applications

Unspecified application

Species

Unspecified reactive species

Xinxin Li,Bihui Yang,Li Wang,Liping Chen,Xiaohua Luo,Lin Liu

Oncotarget 7:51393-51407 PubMed27286445

2016

MiR-7a is an important mediator in Fas-associated protein with death domain (FADD)-regulated expression of focal adhesion kinase (FAK).

Applications

WB

Species

Unspecified reactive species

Yingting Liu,Hongen Cui,Xianjie Huang,Bo Zhu,Shengwen Guan,Wei Cheng,Yueyang Lai,Xiaoxin Zhang,Zi-Chun Hua

Journal of neuroinflammation 9:172 PubMed22789015

2012

P2X7 signaling promotes microsphere embolism-triggered microglia activation by maintaining elevation of Fas ligand.

Applications

WB

Species

Mouse

Ying-mei Lu,Rong-rong Tao,Ji-yun Huang,Li-tao Li,Mei-hua Liao,Xiao-ming Li,Kohji Fukunaga,Ze-Hui Hong,Feng Han
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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