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リコンビナントRabMAb

Anti-Factor XIIIa 抗体 [SP196] - BSA and Azide free (ab245757)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)
  • Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP196] to Factor XIIIa - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Factor XIIIa antibody [SP196] - BSA and Azide free
    Factor XIIIa 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [SP196] to Factor XIIIa - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human placenta, colon, HK lymphoma, esophagus, bladder, lung, skin, skin squamous cell carcinoma, and Human Hodgkins lymphoma tissue.

  • 特記事項

    ab245757 is the carrier-free version of ab225722.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab245757の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.Primary incubation for 20 minutes at room temperature.

 
特記事項
IHC-P
Use at an assay dependent concentration.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.Primary incubation for 20 minutes at room temperature.

 

ターゲット情報

  • 機能

    Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.

  • 関連疾患

    Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.

  • 配列類似性

    Belongs to the transglutaminase superfamily. Transglutaminase family.

  • 翻訳後修飾

    The activation peptide is released by thrombin.

  • 細胞内局在

    Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.
  • Information by UniProt

  • 参照データベース

    • 別名

      • bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody
      • Coagulation factor XIII A chain antibody
      • Coagulation factor XIII A1 polypeptide antibody
      • Coagulation factor XIII A1 subunit antibody
      • Coagulation factor XIII, A polypeptide antibody
      • Coagulation factor XIIIa antibody
      • F13A antibody
      • F13A_HUMAN antibody
      • F13a1 antibody
      • Factor XIIIA antibody
      • Fibrin stabilizing factor, A subunit antibody
      • Fibrinoligase antibody
      • FSF, A subunit antibody
      • Protein glutamine gamma glutamyltransferase A chain antibody
      • Protein-glutamine gamma-glutamyltransferase A chain antibody
      • TGase antibody
      • Transglutaminase A chain antibody
      • Transglutaminase, plasma antibody
      • Transglutaminase. plasma antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Hodgkin's lymphoma tissue sections labeling Factor XIIIa with ab225722 at 1/100 dilution (1.22µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225722)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling Factor XIIIa with ab225722 at 1/100 dilution (1.22 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225722)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)

      Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for Factor XIIIa using ab225722 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS BSA and sodium azide (ab225722).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)

      Formalin-fixed, paraffin-embedded human skin tissue stained for Factor XIIIa using ab225722 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab225722).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)

      Formalin-fixed, paraffin-embedded human lung tissue stained for Factor XIIIa using ab225722 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab225722).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)

      Formalin-fixed, paraffin-embedded human bladder tissue stained for Factor XIIIa using ab225722 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab225722).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)

      Formalin-fixed, paraffin-embedded human esophagus tissue stained for Factor XIIIa using ab225722 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab225722).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)

      Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for Factor XIIIa using ab225722 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab225722).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)

      Formalin-fixed, paraffin-embedded human colon tissue stained for Factor XIIIa using ab225722 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab225722).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)

      Formalin-fixed, paraffin-embedded human placenta tissue stained for Factor XIIIa using ab225722 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab225722).

    • Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)
      Anti-Factor XIIIa antibody [SP196] - BSA and Azide free (ab245757)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab245757 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab245757 は論文での使用が確認できていません。

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com