Anti-Factor XIIIa 抗体 [AC-1A1] (ab1834)

Thank you for your phone call and email.

I apologize as I forget to attach the questionnaire. I have looked into the details and would like to make following suggestions;

ab1834 was able to detect band in Fibrogammin samples so I assume the antibody is fine. The problem might with the plasma samples or the protocol. We have not tested this antibody with plasma sample so we may not be able to recommend you specific protocol.

Factor X111 is present in plasma as well as in platelets. Please use normal plasma along with plasma without the platelets (sample of your interest). Placental tissue lysates can also be used as positive control.

Try increasing the antibody concentration.

In the following publication (http://ard.bmj.com/content/53/4/256.full.pdf+html) researcher have tried non-reduced conditions with plasma samples, you can try this.

Check the protein transfer using ponceau method.

I hope these suggestions would be helpful Should the result do not improve please call us back again we be happy to help.

Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on the phone, I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.

Thank you for your email.

This antibody was used in the following publication:

Ulici V et al. Regulation of gene expression by PI3K in mouse growth plate chondrocytes. PLoS One 5:e8866 (2010). WB, IHC-P; Mouse. (PubMed: 20111593)



They used this protocol with ab1834 with success:

3% H2O2 for 15 min at room temperature, followed by incubation in preheated (2 min at 100°C) 10 mM sodium citrate solution (pH 6.0) for 30 min at 97°C. They were then blocked with 5% serum. Sections were incubated with 0.10 mg/ml factor XIIIa primary antibody over night at 4°C.

General information on IHC-P protocl can be found on our extensive protocols page:

https://www.abcam.com/index.html?pageconfig=popular_protocols

Please feel free to contact me with any further questions.

Thank you for your reply.

As ab125687 PAI1 antibody [1D5], ab1834 Factor XIIIa antibody [AC-1A1] and ab54488 CD41/Integrin alpha 2b antibody [283.16B7] are all mouse IgG1, Ican suggest to consider the following anti mouse IgG1 secondaries available in our catalog. These are biotin conjugated and have been tested and covered by our guarantee for IHC-Fr. I can recommend to review the online datasheets for further information and ensure they are suitable for your purposes:

ab98691 Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (Biotin), pre-adsorbed
Biotin conjugated
Tested in: ELISA, ICC/IF, IHC-P, WB
Reacts with: Mouse
https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-Biotin-preadsorbed-ab98691.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-Biotin-preadsorbed-ab98691.html).

ab97238 Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (Biotin)
Biotin conjugated ELISA, ICC/IF, IHC-P, WB
Reacts with: Mouse
https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-Biotin-ab97238.html (or use the following: https://www.abcam.com/Goat-Mouse-IgG1-heavy-chain-Biotin-ab97238.html).


Once you have decided which of these you would like to order, please email my colleague Andrew again (mailto:andrew.richards@abcam.com) with your choice and he will be pleased to place the order for you.

Please note I am out of the office next week, so I can suggest to contact Andrew if you have any further questions regarding the discounts or the order.

The scientific support team are happy to help if you have any further technical questions (mailto:technical@abcam.com).

I hope this provides a satisfactory outcome for you and I wish you a good bank holiday weekend. Good luck with your research.

Thank you for your enquiry. Further to our liveperson discussion yesterday morning I have performed some literature searching and in the following publication factor XIIIA was studied using BOTH reducing and non-reducing conditions. Whilst it is chicken factor XIIIA I consider it your best option that you electrophorese this protein under both conditions to determine homo- and hetero-dimer formation as they have in the publication: Chicken coagulation factor XIIIA is produced by the theca externa and stabilizes the ovarian follicular wall. J Biol Chem. 2000 Nov 10;275(45):35320-7. http://www.jbc.org/cgi/content/full/275/45/35320 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry regarding ab1834 and your interest in our products. This particular antibody to Factor XIIIa does not require any antigen retrieval for paraffin sections. Proteinase K is more suitable for in situ hybridization and not for IHC. Generally for IHC, the safest enzymatic treatment is Trypsin or Pepsin.

Thank you for your email. I would suggested diluting ab1834 to 1:1000 and optimizing based upon your results. We're unfortunately not aware of any publications which feature the use of this antibody. If you have any additional questions, please do contact us again.

Thank you for your enquiry. You do not need any pretreatment when staining with this particular antibody. It just works fine. However, if your tissue is over fixed, you need enzymatic treatment.

Recombinant protein was purified using HPLC.

The concentration is 100 ug/ml.