Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-Factor H 抗体 [OX-24] (ab118820)

Submit a review Q&A (5)References (8)
Western blot - Anti-Factor H antibody [OX-24] (ab118820)
  • ELISA - Anti-Factor H antibody [OX-24] (ab118820)

Key features and details

  • Mouse monoclonal [OX-24] to Factor H
  • Suitable for: ELISA, WB
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Factor H antibody [OX-24]
    Factor H 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [OX-24] to Factor H

  • 由来種

    Mouse

  • アプリケーション

    適用あり: ELISA, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Full length native protein (purified) corresponding to Human Factor H.

  • ポジティブ･コントロール

    • WB: Human plasma, Human serum, Purified Factor H protein

  • 特記事項

    ab118820 has switched from ascites to TCS on 19th September 2019. Lot numbers higher than GR3258447 are from tissue culture supernatant.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein G purified

  • 特記事項（精製）

    Purified from TCS.

  • ポリ/モノ

    モノクローナル

  • クローン名

    OX-24

  • アイソタイプ

    IgG1

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab118820の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use a concentration of 10 µg/ml.
WB
Use at an assay dependent concentration. Predicted molecular weight: 139 kDa.
特記事項
ELISA
Use a concentration of 10 µg/ml.
WB
Use at an assay dependent concentration. Predicted molecular weight: 139 kDa.

ターゲット情報

  • 機能

    Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b)NBB complex (C5 convertase) in the alternative complement pathway.

  • 組織特異性

    Expressed by the liver and secreted in plasma.

  • 関連疾患

    Genetic variations in CFH are associated with basal laminar drusen (BLD) [MIM:126700]; also known as drusen of Bruch membrane or cuticular drusen or grouped early adult-onset drusen. Drusen are extracellular deposits that accumulate below the retinal pigment epithelium on Bruch membrane. Basal laminar drusen refers to an early adult-onset drusen phenotype that shows a pattern of uniform small, slightly raised yellow subretinal nodules randomly scattered in the macula. In later stages, these drusen often become more numerous, with clustered groups of drusen scattered throughout the retina. In time these small basal laminar drusen may expand and ultimately lead to a serous pigment epithelial detachment of the macula that may result in vision loss.
    Defects in CFH are the cause of complement factor H deficiency (CFH deficiency) [MIM:609814]. CFH deficiency determines uncontrolled activation of the alternative complement pathway with consumption of C3 and often other terminal complement components. It is associated with a number of renal diseases with variable clinical presentation and progression, including membranoproliferative glomerulonephritis and atypical hemolytic uremic syndrome. CFH deficiency patients may show increased susceptibility to meningococcal infections.
    Defects in CFH are a cause of susceptibility to hemolytic uremic syndrome atypical type 1 (AHUS1) [MIM:235400]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
    Genetic variation in CFH is associated with age-related macular degeneration type 4 (ARMD4) [MIM:610698]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid (known as drusen) that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.

  • 配列類似性

    Contains 20 Sushi (CCP/SCR) domains.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • adrenomedullin binding protein antibody
      • age related maculopathy susceptibility 1 antibody
      • AHUS 1 antibody
      • AHUS1 antibody
      • AMBP 1 antibody
      • AMBP1 antibody
      • ARMD 4 antibody
      • ARMD4 antibody
      • ARMS 1 antibody
      • ARMS1 antibody
      • beta 1 H globulin antibody
      • beta 1H antibody
      • beta1H antibody
      • CFAH_HUMAN antibody
      • CFH antibody
      • CFHL 3 antibody
      • CFHL3 antibody
      • Complement factor H antibody
      • complement factor H, isoform b antibody
      • Factor H antibody
      • factor H like 1 antibody
      • FH antibody
      • FHL 1 antibody
      • FHL1 antibody
      • H factor 1 (complement) antibody
      • H factor 1 antibody
      • H factor 2 (complement) antibody
      • HF 1 antibody
      • HF 2 antibody
      • HF antibody
      • HF1 antibody
      • HF2 antibody
      • HUS antibody
      • MGC88246 antibody
      see all

    画像

    • Western blot - Anti-Factor H antibody [OX-24] (ab118820)
      Western blot - Anti-Factor H antibody [OX-24] (ab118820)
      All lanes : Anti-Factor H antibody [OX-24] (ab118820) at 1 µg/ml

      Lane 1 : Human serum diluted 1/100 at 10 µl
      Lane 2 : Human plasma diluted 1/100 at 10 µl
      Lane 3 : Purified Factor H protein at 0.5 µg
      Lane 4 : Purified Factor H protein at 0.1 µg

      Performed under reducing conditions.

      Predicted band size: 139 kDa
      Observed band size: 170 kDa why is the actual band size different from the predicted?



      This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab118820 overnight at 4°C at a 1ug/ml concentration. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) at 1/20000 dilution for 1 hour at room temperature before imaging.

      This image was generated using the ascites version of the product.

    • ELISA - Anti-Factor H antibody [OX-24] (ab118820)
      ELISA - Anti-Factor H antibody [OX-24] (ab118820)

      96-well microtitre plates were coated overnight at 4°C with recombinant human C3, C3b, iC3b, and Factor H proteins, in duplicate at a concentration of 1µg/mL. Plates were blocked with 1% BSA in PBS-T (0.1% Tween®) for 1 hour before incubation with a 10-step 4x serial dilution of ab118820 from 10µg/mL for 1 hour at room temperature. Antibody binding was detected with Goat Anti-Mouse IgG H&L (HRP) (ab6789) secondary antibody at a 1 in 10000 dilution for 1 hour at room temperature. Plates were incubated with TMB ELISA substrate for 7 minutes prior to being stopped with Stop solution and absorbance measured at 450nm.

    参考文献 (8)

    ab118820 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab118820 は 8 報の論文で使用されています。

    • Xing Z  et al. The Reduction of Uromodulin, Complement Factor H, and Their Interaction Is Associated with Acute Kidney Injury to Chronic Kidney Disease Transition in a Four-Time Cisplatin-Injected Rat Model. Int J Mol Sci 24:N/A (2023). PubMed: 37047611
    • Li J  et al. Complement factor H inhibits endothelial cell migration through suppression of STAT3 signaling. Exp Ther Med 26:408 (2023). PubMed: 37522066
    • Sun Y  et al. Factor H Is Bound by Outer Membrane-Displayed Carbohydrate Metabolism Enzymes of Extraintestinal Pathogenic Escherichia coli and Contributes to Opsonophagocytosis Resistance in Bacteria. Front Cell Infect Microbiol 10:592906 (2020). PubMed: 33569353
    • Chen X  et al. Cadmium Induces Glomerular Endothelial Cell-Specific Expression of Complement Factor H via the -1635 AP-1 Binding Site. J Immunol 202:1210-1218 (2019). PubMed: 30642982
    • Medjeral-Thomas NR  et al. Glomerular Complement Factor H-Related Protein 5 (FHR5) Is Highly Prevalent in C3 Glomerulopathy and Associated With Renal Impairment. Kidney Int Rep 4:1387-1400 (2019). PubMed: 31701048
    • Liu J & Hoh J Loss of Complement Factor H in Plasma Increases Endothelial Cell Migration. J Cancer 8:2184-2190 (2017). WB ; Mouse . PubMed: 28819420
    • Popek S  et al. IL-6 and IL-8 enhance factor H binding to the cell membranes. Mol Med Rep 13:3886-94 (2016). IF . PubMed: 27035765
    • Lee MS  et al. Exploitation of the complement system by oncogenic Kaposi's sarcoma-associated herpesvirus for cell survival and persistent infection. PLoS Pathog 10:e1004412 (2014). ICC/IF ; Human . PubMed: 25254972

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Question

    Please find my answers in the questionnaire.
    Thanks


    1) Abcam product code: ab 118820 Lot: GR19604-5
    2) Abcam order reference number: ??????
    3) Description of the problem:
    The anti-GPCR GPR49 antibody showed no staining at all. Our technicians are highly experienced in establishing new antibodies (having far over 1000 antibodies established in the last years). Lot-Nr. GR19604-5
    4) Sample preparation:
    Species : different murine and human tissues
    Type of sample: formalin fixed paraffin embedded sections
    Sample preparation:
    Positive control: murine gut, brain, human plazenta
    Negative control Multitissue-Array with human normal and tumor samples
    5) Fixation step
    Yes
    If yes: 10% buffered Formalin
    Fixation time: several days
    Fixation temperature: room temperature
    6) Antigen retrieval method
    HIER with EDTA-Buffer pH8, Citrate-Buffer pH6,0, different intensities
    7) Permeabilization method:
    Did you do a permeabilization step (details please) or add permeabilizing agent in any dilution buffers?
    Permeabilizing agent and concentration: Detergents in commercial LEICA-Washing-buffer
    8) Blocking agent (eg BSA, serum…):
    Concentration
    Blocking time
    Blocking temperature
    9) Endogenous peroxidases blocked?
    Endogenous biotins blocked?
    LEICA- Refine-DAB-Kit
    10) Primary antibody (If more than one was used, describe in additional notes) :
    Concentration or dilution 1:25
    Diluent buffer LEICA-Diluent
    Incubation time 30min.
    11) Secondary antibody:
    Species:
    LEICA-Refine DAB-Kit against Rabbit primary antibodies
    12) Washing after primary and secondary antibodies:
    Buffer: LEICA BondMax
    Number of washes
    13) Detection method
    LEICA Refine-DAB-Kit on BondMax, according to the manufacturers protocols
    14) How many times have you run this staining? Several times
    Do you obtain the same results every time? yes
    What steps have you altered to try and optimize the use of this antibody?
    Several Epitope Retrievals, Different Antibody-Concentrations

    Read More
    Answer

    Thank you for taking the time to complete our questionnaire.

    The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

    I appreciate the experience of your team and the time they have spent on these experiments in the laboratory. I fully understand your concerns and it is disappointing the results have not been successful.

    I would like to reassure you that ab118820 is tested and covered by our 6 month guarantee in IHC-P and human samples. We are happy to offer a refund, credit note or free of charge replacement when a product is not working in a successfully tested applications or species within the guarantee period. However, we do often find that suggesting some scientifically thought out optimisation tips helps to improve the results. So I hope you can understand that we like to offer the best technical support possible to provide a satisfactory outcome.

    Reviewing the details, I would like to offer some suggestions to help optimise the results. I would also appreciate if you can confirm some further details to help with my investigation of this case:

    1. The secondary details state 'LEICA-Refine DAB-Kit against Rabbit primary antibodies' . The ab118820 Factor H antibody is a mouse monoclonal IgG1 primary antibody, and will require an anti mouse secondary antibody for detection. An anti-rabbit secondary will not work for detection of ab118820. Please confirm further details of the secondary antibody.

    2. I can suggest to try overnight at 4oC. This often provides more efficient and specific staining.

    3. Could you confirm if different times of antigen retrieval have been tried? This can sometimes require some optimization. For example, try 2, 5, 10 and 15 minutes?

    I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested information and details of how you would like to proceed.

    Read More

    Question

    Dear technical support team,

    We ordered in Jan 2012 the above mentioned antibody. We took it because we believed in the good quality of abcam antibodies and have seen several good publications using this antibody. Unfortunatly we tested the whole aliquot and were not able to get any staining. We would like to stain “stem” cells from the murine and human colon.

    If you could recommened us another antibody for testing, we would be happy to do so.

    With kind regards,

    Read More
    Answer

    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB, IP, ELISA, IHC-P, Flow Cytometry and in human samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

    I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily. I have enclosed the IHC form as I think this is what you may be using? Please let me know if you are using a different application and I will be pleased to send you the relevant form.

    In addition to this:

    1. Please confirm the order number and date of purchase? Was this Abcam order ref #### purchased 14th March? Purchase order #####?

    2. Regrettably, I can confirm this antibody is not tested in mouse. All tested species covered by the guarantee are listed on the online datasheets. I have reviewed the alignment of the full length human protein which has been used as an immunogen with the mouse protein, and this has only 61% alignment to mouse, which is quite low. I would therefore suggest the antibody would probably not react with mouse.

    3. With regards to suggesting a different antibody to try, I would appreciate if you could confirm confirm which application you are using so I can ensure to select a suitable one for you that is covered by the guarantee.

    I would appreciate if you could also provide an image which would help us to assess the results.

    Thank you for your time and cooperation. We look forward to receiving the completed questionnaire.


    Order Details Antibody code:
    Lot number:
    Purchase order number or preferably Abcam order number:
    General Information Antibody storage conditions (temperature/reconstitution etc)
    Description of the problem (high background, low signal, non-specific staining etc.)
    Sample (Species/Tissue/Cell Type/Cell Line etc.)
    Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)
    Antigen retrieval (Enzymatic method, Heat mediated technique etc.)
    Permeabilization step
    Blocking conditions (Buffer/time period, Blocking agent etc.)
    Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
    Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
    Detection method
    Positive and negative controls used (please specify)
    Optimization attempts (problem solving) How many times have you tried the IHC?
    Have you run a "No Primary" control? Yes No
    Do you obtain the same results every time? Yes No
    What steps have you altered?
    Additional Notes
    We would appreciate if you are also able to provide and image which would help us to assess the results

    Read More

    Question

    1) Abcam product code: ab 118820
    2) Abcam order reference number: x

    3) Description of the problem:

    The Anti-Factor H antibody shows no specific staining. The positive control (liver tissue) is also negative (Pictures attached). Our technicians are highly experienced in establishing new antibodies (having far over 1000 antibodies established in the last years). The other ordered and newly established Anti-Carboxypeptidase M antibody works fine.

    4) Sample preparation:
    Species : TMA with liver, lymph node, lung, uterus, testis, kidney, prostate
    Type of sample: formalin fixed paraffin embedded sections
    Sample preparation:
    Positive control: liver tissue
    Negative control

    5) Fixation step
    Yes
    If yes: 10% buffered Formalin
    Fixation time: several days
    Fixation temperature: room temperature

    6) Antigen retrieval method
    HIER with EDTA-Buffer pH8, Citrate-Buffer pH6,0, different times
    PIER: Proteinase K different times

    7) Permeabilization method:
    Did you do a permeabilization step (details please) or add permeabilizing agent in any dilution buffers?
    Permeabilizing agent and concentration: Detergents in commercial LEICA-Washing-buffer

    8) Blocking agent (eg BSA, serum…):
    Concentration
    Blocking time
    Blocking temperature

    9) Endogenous peroxidases blocked?
    Endogenous biotins blocked?
    LEICA- Refine-DAB-Kit

    10) Primary antibody (If more than one was used, describe in “additional notes”) :
    Concentration or dilution 1:100
    Diluent buffer LEICA-Diluent
    Incubation time 30min.

    11) Secondary antibody:
    Species:
    LEICA-Refine DAB-Kit against Rabbit and Mouse primary antibodies

    12) Washing after primary and secondary antibodies:
    Buffer: LEICA BondMax
    Number of washes

    13) Detection method

    LEICA Refine-DAB-Kit on BondMax, according to the manufacturers protocols

    14) How many times have you run this staining? 3times
    Do you obtain the same results every time? yes
    What steps have you altered to try and optimize the use of this antibody? Different Pretreatment, Antibody-Concentrations

    Document attachment: Attaching images of your IHC is strongly recommended and can greatly speed up our investigation of your problem.

    Read More
    Answer

    Vielen Dank für Ihre Antwort und für diese weiteren Informationen.

    Es tut mir leid zu hören, dass der Factor H-Antikörper ab118820nicht so funktioniert hat, wie auf dem Datenblatt beschrieben (d.h. in einer Konzentration von 20 µg/ml nach HIER mit Citratpuffer pH6). Die Protokolle mit den unterschiedlichen Demaskierungsmethoden, die Sie verwendet haben,sind demProtokoll, nach demwir die Antikörper testen (s. Anhang),in der Tat sehr ähnlich. Ich weiß, dass Sie viel Zeit für diese Experimente verwendet haben und möchte Ihnen daher einen Ersatz oder eineGutschrift anbieten:

    Möchten Sie noch einmal ein Röhrchen von ab118820, oder benötigen Sie einen anderen primären Antikörper?

    Oder würden Sie eine Gutschrift bevorzugen?

    Vielen Dank für Ihre Kooperation und Hilfsbereitschaft. Ich freue mich bald wieder von Ihnen zu hören.

    Read More

    Question

    phone call: no signal in IHC

    Read More
    Answer

    Vielen Dank für Ihren Anruf.

    Es tut mir leid zu hören, dass Sie Probleme mit diesem Antikörper haben.

    Wie am Telefon besprochen, sende ich Ihnen hier unseren Fragebogen im Anhang. Durch das Ausfüllen des Fragebogens erhalten wir alle nötigen Informationen über Ihre Proben und Ihr Protokoll. Sobald Sie dieses Formular an uns zurückgeschickt haben, werden wir uns Ihr Protokoll ansehen und möglichst Veränderungsvorschläge machen, die Ihre Ergebnisse verbessern werden. Falls sich herausstellt, dass der Antikörper nicht so funktioniert, wie auf dem Datenblatt beschrieben, werden wir Ihnen gerne einen Ersatz oder eine Gutschrift schicken.

    Ich freue mich, bald wieder von Ihnen zu hören.

    Read More

    Question

    Dear Sir or Madam, as planning to perform IHC on factor H in mouse tissue, I would like you to inform me whether the 2 antibodies ab118820 and ab112197 are basically the same with the difference of biotin-labelling and no testing so far for IHC of ab112197? Thank you.

    Read More
    Answer

    Thank you for contacting us.
    Both antibodies are derived from the same clone, OX-24. Not only is one conjugated, the buffer components are slightly different as well. However, even if clones do sometimes show different behaviour regarding reactivity and application form, most of the time they react identical. And yes, ab112197 hasn't been tested in IHC yet.
    If you are interested in doing so, I could make you a special offer in this case.
    Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com