Anti-Factor D/CFD 抗体 [EPR17004-285] (ab181307)
![Western blot - Anti-Factor D/CFD antibody [EPR17004-285] (ab181307)](https://www.abcam.co.jp/ps/products/181/ab181307/Images/ab181307-294500-anti-factor-d-antibody-epr17004-285-western-blot.jpg "Western blot - Anti-Factor D/CFD antibody [EPR17004-285] (ab181307)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17004-285] to Factor D/CFD
- Suitable for: WB
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-Factor D/CFD antibody [EPR17004-285]
Factor D/CFD 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR17004-285] to Factor D/CFD -
由来種
Rabbit -
特異性
This antibody detects a distinct epitope compared to ab213177. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: His-tagged Mouse Factor D/CFD (aa26-259) recombinant protein. Mouse plasma, serum and lung lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR17004-285 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181307の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 27 kDa.
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| 特記事項 |
|---|
|
WB
1/1000. Predicted molecular weight: 27 kDa. |
ターゲット情報
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機能
Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. -
関連疾患
Defects in CFD are the cause of complement factor D deficiency (CFD deficiency) [MIM:134350]. CFD deficiency predisposes to invasive meningococcal disease. -
配列類似性
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 11537 Mouse
- SwissProt: P03953 Mouse
- Unigene: 4407 Mouse
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別名
- Adipsin antibody
- Adipsin/complement factor D antibody
- Adn antibody
see all
画像
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Western blot - Anti-Factor D/CFD antibody [EPR17004-285] (ab181307)Anti-Factor D/CFD antibody [EPR17004-285] (ab181307) at 1/100000 dilution + His-tagged Mouse Factor D/CFD (aa26-259) recombinant protein 10 ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/ Dilution buffer and concentration: 5% NFDM/TBST.
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![Western blot - Anti-Factor D/CFD antibody [EPR17004-285] (ab181307)](https://www.abcam.co.jp/ps/products/181/ab181307/Images/ab181307-294499-anti-factor-d-antibody-epr17004-285-western-blot.jpg)
Western blot - Anti-Factor D/CFD antibody [EPR17004-285] (ab181307)All lanes : Anti-Factor D/CFD antibody [EPR17004-285] (ab181307) at 1/1000 dilution
Lane 1 : Mouse plasma lysate
Lane 2 : Mouse serum lysate
Lane 3 : Mouse lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa
Observed band size: 45-50 kDa why is the actual band size different from the predicted?Blocking/ Dilution buffer and concentration: 5% NFDM/TBST.
Exposure times:
Lane 1/2: 1 second
Lane 3: 15 seconds
The higher molecular mass corresponds to glycosylated forms of the protein. The expression profile observed is consistent with what has been described in the literature (PMID: 20038603; PMID: 12949072).
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![Anti-Factor D/CFD antibody [EPR17004-285] (ab181307)](https://www.abcam.co.jp/ps/products/181/ab181307/Images/ab181307-3-benefits-of-recombinant-antibodies.png)
Anti-Factor D/CFD antibody [EPR17004-285] (ab181307)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab181307 は論文での使用が確認できていません。