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AB302722

Anti-Factor B 抗体 [EPR24245-109] - BSA and Azide free (Capture)

Anti-Factor B antibody [EPR24245-109] - BSA and Azide free (Capture)

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Anti-Factor B antibody [EPR24245-109] - BSA and Azide free (Capture) (ab302722) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Factor B in ELISA. Suitable for Human.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

別名を表示する

BF, BFD, CFB, Complement factor B, C3/C5 convertase, Glycine-rich beta glycoprotein, PBF2, Properdin factor B, GBG

1 Images
Sandwich ELISA - Anti-Factor B antibody [EPR24245-109] - BSA and Azide free (Capture) (AB302722)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Factor B antibody [EPR24245-109] - BSA and Azide free (Capture) (AB302722)

Sandwich ELISA of ab302721 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.25 µg/mL.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR24245-109

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Human

アプリケーション

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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製品の詳細

ab302722 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Factor B. The recommended pair for sandwich ELISA is:
     Capture: ab302722, Human Factor B Capture Antibody (unconjugated)
     Detector: ab302723, Human Factor B Detector Antibody (unconjugated)
The reference range value is 31.2-2000pg/ml.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Factor B also known as B-factor is a protein that plays an important role in the immune system specifically in the complement pathway. It is sometimes referred to as factor B complement and its gene identifier is often referenced as KY21 NNL or factor B 1001. Factor B has a molecular mass of approximately 93 kDa. It is mainly expressed in the liver but can also be found in blood plasma in its inactive form as part of the innate immune response.
Biological function summary

Factor B is involved in the alternative complement pathway which serves as a part of the body's defense mechanism. It participates in the formation of the C3 convertase together with factor D and properdin initiating a cascade that can lead to the opsonization and elimination of pathogens. Factor B undergoes cleavage by factor D which generates the Ba and Bb fragments with Bb functioning as a serine protease within the C3 convertase complex.

Pathways

Factor B contributes to the complement system interacting notably in the alternative and lectin pathways. In these pathways Factor B associates with proteins like complement component C3. The alternative pathway is activated on microbial surfaces independent of antibodies. The involvement of Factor B in these pathways illustrates its role in pathogen recognition and further highlights its cooperation with complement proteins such as C3 and properdin.

Factor B has been associated with atypical hemolytic uremic syndrome (aHUS) a rare and serious disease affecting kidney function. Factor B involvement in aHUS links it to abnormal complement activation leading to a pathological condition. Additionally Factor B connects to age-related macular degeneration (AMD) a disorder where complement pathway genetic variations including those involving Factor B have shown associations. Understanding interactions with other complement components such as factor H helps to elucidate its role in these diseases.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Precursor of the catalytic component of the C3 and C5 convertase complexes of the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). The alternative complement pathway acts as an amplification loop that enhances other complement pathways (classical, lectin and GZMK) by promoting formation of additional C3 and C5 convertases (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). CFB is cleaved and activated by CFD to generate Ba and Bb chains; Bb chain constituting the catalytic component of the C3 and C5 convertases (PubMed : 6769474, PubMed : 9748277).. Complement factor B Bb. Serine protease component of the complement C3 and C5 convertase complexes of the alternative complement pathway (PubMed : 30643019, PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543). Following cleavage and activation by factor D (CFD), forms the C3 convertase together with complement C3b (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). As part of the C3 convertase, cleaves and activates C3 into C3a anaphylatoxin and C3b opsonin, the next components of the complement pathways (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). When an additional complement C3b molecule binds to the C3 convertase, forms the C5 convertase, which cleaves and activates C5 into C5a anaphylatoxin and C5b component of the membrane attack complex (PubMed : 30643019, PubMed : 624565, PubMed : 6554279).. Complement factor B Ba. Involved in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes.
See full target information CFB
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