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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-FABP5 抗体 [EPR22552-64] (ab255276)

Submit a review Submit a question References (7)
Immunocytochemistry/ Immunofluorescence - Anti-FABP5 antibody [EPR22552-64] (ab255276)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] (ab255276)
  • Western blot - Anti-FABP5 antibody [EPR22552-64] (ab255276)
  • Western blot - Anti-FABP5 antibody [EPR22552-64] (ab255276)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] (ab255276)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] (ab255276)
  • Anti-FABP5 antibody [EPR22552-64] (ab255276)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22552-64] to FABP5
  • Suitable for: ICC/IF, WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-FABP5 antibody [EPR22552-64]
    FABP5 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR22552-64] to FABP5

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WB, IHC-Pmore details
    適用なし: ChIP,Flow Cyt or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: MDA-MB-231, HeLa and PC-3 whole cell lysates; IHC-P: Human placenta, cerebral cortex and skin tissue. ICC/IF: MDA-MB-231 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab255276の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/50.
WB
1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
ICC/IF
1/50.
WB
1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
追加情報
Is unsuitable for ChIP,Flow Cyt or IP.

ターゲット情報

  • 機能

    High specificity for fatty acids. Highest affinity for C18 chain length. Decreasing the chain length or introducing double bonds reduces the affinity. May be involved in keratinocyte differentiation.

  • 組織特異性

    Keratinocytes; highly expressed in psoriatic skin.

  • 配列類似性

    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.

  • ドメイン

    Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CFABP antibody
      • Cutaneous fatty acid binding protein antibody
      • DA11 antibody
      • Differentiation associated lipid binding protein LP2 antibody
      • E-FABP antibody
      • EFABP antibody
      • Epidermal-type fatty acid-binding protein antibody
      • FABP5 antibody
      • FABP5_HUMAN antibody
      • fatty acid binding protein 5 (psoriasis-associated) antibody
      • Fatty acid binding protein epidermal antibody
      • Fatty acid-binding protein 5 antibody
      • Fatty acid-binding protein, epidermal antibody
      • Keratinocyte lipid binding protein antibody
      • KFABP antibody
      • Klbp antibody
      • mal1 antibody
      • PA-FABP antibody
      • PAFABP antibody
      • Psoriasis associated fatty acid binding protein homolog antibody
      • Psoriasis-associated fatty acid-binding protein homolog antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-FABP5 antibody [EPR22552-64] (ab255276)
      Immunocytochemistry/ Immunofluorescence - Anti-FABP5 antibody [EPR22552-64] (ab255276)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell) labeling FABP5 with ab255276 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 was used as a counterstain (red).

      The nuclear counterstain is DAPI (blue).

      Confocal image showing cytoplasmic and nuclear staining in MDA-MB-231 cells.
      Negative control: T-47D (PMID: 21356353).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] (ab255276)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] (ab255276)

      Immunohistochemical analysis of paraffin-embedded human skin tissue labeling FABP5 with ab255276 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human skin (PMID: 8092987) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

       

    • Western blot - Anti-FABP5 antibody [EPR22552-64] (ab255276)
      Western blot - Anti-FABP5 antibody [EPR22552-64] (ab255276)
      All lanes : Anti-FABP5 antibody [EPR22552-64] (ab255276) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : FABP5 knockout HeLa cell lysate
      Lane 3 : PC-3 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 15 kDa
      Observed band size: 17 kDa why is the actual band size different from the predicted?



      Lanes 1-3: Merged signal (red and green). Green - ab255276 observed at 17 kDa. Red - loading control ab8245 observed at 36 kDa. 

       ab255276 Anti-FABP5 antibody [EPR22552-64] was shown to specifically react with FABP5 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265905 (knockout cell lysate ab257431) was used.  Wild-type and FABP5 knockout samples were subjected to SDS-PAGE.  ab255276 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-FABP5 antibody [EPR22552-64] (ab255276)
      Western blot - Anti-FABP5 antibody [EPR22552-64] (ab255276)
      All lanes : Anti-FABP5 antibody [EPR22552-64] (ab255276) at 1/1000 dilution

      Lane 1 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 3 : PC-3 (human prostate adenocarcinoma epithelial cell line) whole cell lysate
      Lane 4 : T-47D (human ductal breast epithelial tumor cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 15 kDa
      Observed band size: 15 kDa


      Exposure time: 8 seconds


      Blocking and dilution buffer: 5% NFDM/TBST.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 25260874).

      Negative control: T47D (PMID: 21356353).

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] (ab255276)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] (ab255276)

      Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling FABP5 with ab255276 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human cerebral cortex (PMID: 24114376) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] (ab255276)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] (ab255276)

      Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling FABP5 with ab255276 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on blood vessels of human placenta (PMID: 19625659) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • Anti-FABP5 antibody [EPR22552-64] (ab255276)
      Anti-FABP5 antibody [EPR22552-64] (ab255276)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (7)

    ab255276 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab255276 は 7 報の論文で使用されています。

    • Chen XJ  et al. Lipid droplets' functional protein caveolin-2 is associated with lipid metabolism-related molecule FABP5 and EMT marker E-cadherin in oral epithelial dysplasia. J Clin Pathol 77:330-337 (2024). PubMed: 36854623
    • Kang Z  et al. Whole genome methylation sequencing reveals epigenetic landscape and abnormal expression of FABP5 in extramammary Paget's disease. Skin Res Technol 29:e13497 (2023). PubMed: 37881057
    • Zhang G  et al. A Novel Molecular Classification Method for Glioblastoma Based on Tumor Cell Differentiation Trajectories. Stem Cells Int 2023:2826815 (2023). PubMed: 37964983
    • Gao Y  et al. Establishment and characterization of adult human gastric epithelial progenitor-like cell lines. Cell Prolif 56:e13355 (2023). PubMed: 36331058
    • Tao L  et al. CD36 accelerates the progression of hepatocellular carcinoma by promoting FAs absorption. Med Oncol 39:202 (2022). PubMed: 36175596
    • Voigt AP  et al. Human photoreceptor cells from different macular subregions have distinct transcriptional profiles. Hum Mol Genet 30:1543-1558 (2021). PubMed: 34014299
    • Li M  et al. Expression and function analysis of CRABP2 and FABP5, and their ratio in esophageal squamous cell carcinoma. Open Med (Wars) 16:1444-1458 (2021). PubMed: 34632074

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