Anti-FABP5 抗体 (ab84028)
Key features and details
- Rabbit polyclonal to FABP5
- Suitable for: ICC/IF, IP, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-FABP5 antibody
FABP5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FABP5 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IP, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Sheep, Cow, Pig
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免疫原
Synthetic peptide corresponding to Human FABP5 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab94388) -
ポジティブ･コントロール
- WB: HeLa, PC-3, HepG2 and A431 whole cell lystes; Skin tissue lysate. ICC/IF: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98.98% PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab84028の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
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| IP |
Use a concentration of 5 µg/ml.
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| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa).
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 5 µg/ml. |
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IP
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 15 kDa). |
ターゲット情報
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機能
High specificity for fatty acids. Highest affinity for C18 chain length. Decreasing the chain length or introducing double bonds reduces the affinity. May be involved in keratinocyte differentiation. -
組織特異性
Keratinocytes; highly expressed in psoriatic skin. -
配列類似性
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
ドメイン
Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 2171 Human
- Omim: 605168 Human
- SwissProt: Q01469 Human
- Unigene: 408061 Human
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別名
- CFABP antibody
- Cutaneous fatty acid binding protein antibody
- DA11 antibody
see all
画像
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Western blot - Anti-FABP5 antibody (ab84028)All lanes : Anti-FABP5 antibody (ab84028) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : FABP5 knockout HeLa cell lysate
Lane 3 : PC-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 15 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab84028 observed at 17 kDa. Red - loading control ab8245 observed at 36 kDa.
ab84028 Anti-FABP5 antibody was shown to specifically react with FABP5 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265905 (knockout cell lysate ab257431) was used. Wild-type and FABP5 knockout samples were subjected to SDS-PAGE. ab84028 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunocytochemistry/ Immunofluorescence - Anti-FABP5 antibody (ab84028)ICC/IF image of ab84028 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84028, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2, MCF-7 cells at 5µg/ml, and in 100% Methanol fixed (5 min) HeLa, Hek293, HepG2, MCF-7 cells at 5µg/ml. -
Western blot - Anti-FABP5 antibody (ab84028)All lanes : Anti-FABP5 antibody (ab84028) at 1 µg/ml
Lane 1 : Human skin tissue lysate - total protein (ab30166)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Immunoprecipitation - Anti-FABP5 antibody (ab84028)FABP5 was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab84028.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 16kDa; FABP5.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab84028 は 9 報の論文で使用されています。
- Yang M et al. NSUN2 promotes osteosarcoma progression by enhancing the stability of FABP5 mRNA via m5C methylation. Cell Death Dis 14:125 (2023). PubMed: 36792587
- Tang Y et al. Fatty acid binding protein 5 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by degradation of Krüppel-like factor 9 mediated by miR-889-5p via cAMP-response element binding protein. Cancer Biol Ther 23:424-438 (2022). PubMed: 35816613
- Wang Y et al. FABP5 enhances malignancies of lower-grade gliomas via canonical activation of NF-?B signaling. J Cell Mol Med 25:4487-4500 (2021). PubMed: 33837625
- Wu H et al. CircARL8B Contributes to the Development of Breast Cancer Via Regulating miR-653-5p/HMGA2 Axis. Biochem Genet N/A:N/A (2021). PubMed: 34050452
- Layton TB et al. Cellular census of human fibrosis defines functionally distinct stromal cell types and states. Nat Commun 11:2768 (2020). PubMed: 32488016
- Zaatiti H et al. Tumorigenic proteins upregulated in the MYCN-amplified IMR-32 human neuroblastoma cells promote proliferation and migration. Int J Oncol 52:787-803 (2018). PubMed: 29328367
- Li M et al. Comparative iTRAQ proteomics revealed proteins associated with horn development in yak. Proteome Sci 16:14 (2018). PubMed: 30061793
- Lu JB et al. Altered epidermal fatty acid-binding protein expression in hepatocellular carcinoma predicts unfavorable outcomes. Cancer Manag Res 10:6275-6284 (2018). PubMed: 30538573
- Suojalehto H et al. Level of Fatty Acid Binding Protein 5 (FABP5) Is Increased in Sputum of Allergic Asthmatics and Links to Airway Remodeling and Inflammation. PLoS One 10:e0127003 (2015). PubMed: 26020772