JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB54615

Anti-FAAH1 抗体 [4H8]

Anti-FAAH1 antibody [4H8]

5

(6 Reviews)

|

(31 Publications)

Mouse Monoclonal FAAH1 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 31 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FAAH aa 450 to C-terminus.

別名を表示する

FAAH1, FAAH, Fatty-acid amide hydrolase 1, Anandamide amidohydrolase 1, Fatty acid ester hydrolase, Oleamide hydrolase 1

5 Images
Immunoprecipitation - Anti-FAAH1 antibody [4H8] (AB54615)
  • IP

Unknown

Immunoprecipitation - Anti-FAAH1 antibody [4H8] (AB54615)

FAAH1 was immunoprecipitated using 0.5mg Mouse Liver whole tissue extract, 5μg of Mouse monoclonal to FAAH1 and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver whole tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70oC; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab54615.
Secondary : Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band : 63kDa : FAAH1.

This image was generated using the ascites version of the product.

All lanes:

Immunoprecipitation - Anti-FAAH1 antibody [4H8] (ab54615)

Predicted band size: 63 kDa

false

Western blot - Anti-FAAH1 antibody [4H8] (AB54615)
  • WB

Unknown

Western blot - Anti-FAAH1 antibody [4H8] (AB54615)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-FAAH1 antibody [4H8] (ab54615)

All lanes:

NIH/3T3

Predicted band size: 63 kDa

false

Western blot - Anti-FAAH1 antibody [4H8] (AB54615)
  • WB

Unknown

Western blot - Anti-FAAH1 antibody [4H8] (AB54615)

FAAH antibody (ab54615) at 1ug/lane + A-431 cell lysate at 25ug/lane.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-FAAH1 antibody [4H8] (ab54615)

Predicted band size: 63 kDa

false

Western blot - Anti-FAAH1 antibody [4H8] (AB54615)
  • WB

Unknown

Western blot - Anti-FAAH1 antibody [4H8] (AB54615)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-FAAH1 antibody [4H8] (ab54615)

All lanes:

Rat Brain

Predicted band size: 63 kDa

false

Western blot - Anti-FAAH1 antibody [4H8] (AB54615)
  • WB

Unknown

Western blot - Anti-FAAH1 antibody [4H8] (AB54615)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-FAAH1 antibody [4H8] (ab54615)

All lanes:

PC-12

Predicted band size: 63 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

4H8

アイソタイプ

IgG2a

軽鎖のタイプ

kappa

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

WB, IP, ICC/IF, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human FAAH aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O00519

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>See Abreview.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>See Abreview.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>See Abreview.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

下記製品にもご興味をお持ちいただけるかもしれません:

AB252895

Fatty Acid Amide Hydrolase Activity Assay Kit (Fluorometric)

4

1 Reviews

製品ページを見る
こちらの製品も推奨する理由

この製品は、同様の実験や関連する研究で広く使用されていることから推奨させていただいております。

お客様の実験に適するかご確認いただくため、必ず製品データシートをご確認いただくことをお勧めいたします。ご不明点等ございましたら、テクニカルサポートチームまでお問い合わせください。

style
left-column

製品の詳細

style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製度
Tissue culture supernatant
バッファー組成
pH: 7.4 Constituents: 91% Water, 8% Sodium chloride, 0.6% Disodium hydrogenorthophosphate, 0.2% Potassium chloride, 0.2% Potassium phosphate monobasic
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Fatty acid amide hydrolase 1 (FAAH1) often referred to simply as FAAH is an enzyme that plays a critical role in the degradation of fatty acid amides such as anandamide in the body. This enzyme weighing approximately 63 kDa is a part of the amidase signature family and is characterized by its amidase hydrolase activity. FAAH1 is predominantly expressed in the nervous system liver and gastrointestinal tract where it hydrolyses amide bonds facilitating the termination of signaling pathways initiated by neurotransmitter-like fatty acid amides.
Biological function summary

FAAH1 is essential for maintaining homeostasis by modulating levels of bioactive lipids. It exerts control over endocannabinoid signaling by breaking down anandamide a lipid derivative functioning as a neurotransmitter. FAAH1 does not act as part of a larger protein complex. Instead it operates alone to regulate the duration and intensity of signaling rather than relying on direct interactions within a complex. The enzyme's function impacts the neurochemical milieu in tissues where it is actively expressed thereby affecting mood pain sensation and more.

Pathways

FAAH1 has a significant role in the endocannabinoid signaling pathway and fatty acid metabolism. Within these pathways it modulates signaling by hydrolysing bioactive amides like anandamide influencing overall lipid signaling. FAAH1 activities intersect with several other proteins such as cannabinoid receptors which bind the substrates that FAAH1 eventually degrades. These interactions are vital in controlling signal transduction processes that regulate physiological responses including stress and analgesia.

FAAH1 has been implicated in conditions like chronic pain and anxiety disorders. Altered FAAH1 function or expression can lead to dysregulation of endocannabinoid signaling contributing to the pathophysiology of these conditions. The enzyme's modulation of anandamide levels connects it to proteins such as CB1 and CB2 cannabinoid receptors which mediate the effects of endocannabinoids in neurological and psychiatric disorders. Research into FAAH1 inhibitors as therapeutic agents highlights its importance in potential treatments for managing pain and anxiety.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Catalyzes the hydrolysis of endogenous amidated lipids like the sleep-inducing lipid oleamide ((9Z)-octadecenamide), the endocannabinoid anandamide (N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine), as well as other fatty amides, to their corresponding fatty acids, thereby regulating the signaling functions of these molecules (PubMed : 17015445, PubMed : 19926788, PubMed : 9122178). Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates (PubMed : 17015445, PubMed : 9122178). It can also catalyze the hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol) (PubMed : 21049984). FAAH cooperates with PM20D1 in the hydrolysis of amino acid-conjugated fatty acids such as N-fatty acyl glycine and N-fatty acyl-L-serine, thereby acting as a physiological regulator of specific subsets of intracellular, but not of extracellular, N-fatty acyl amino acids (By similarity).
See full target information FAAH
style
left-column

文献 (31)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 14:910-918 PubMed37921652

2023

FAAH inhibition ameliorates breast cancer in a murine model.

Applications

Unspecified application

Species

Unspecified reactive species

Mallika Tripathy,Amy Bui,Jared Henderson,Jeffrey Sun,Christian Rutan Woods,Soumya Somani,Thao Doan,Anto Sam Crosslee Louis Sam Titus,Chandra Mohan

Brain research 1822:148638 PubMed37858856

2023

Effects of acute Δ-tetrahydrocannabinol on behavior and the endocannabinoid system in HIV-1 Tat transgenic female and male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Barkha J Yadav-Samudrala,Benjamin L Gorman,Hailey Dodson,Shreya Ramineni,E Diane Wallace,Michelle R Peace,Justin L Poklis,Wei Jiang,Sylvia Fitting

International journal of molecular sciences 24: PubMed37686443

2023

Renal Endocannabinoid Dysregulation in Obesity-Induced Chronic Kidney Disease in Humans.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Permyakova,Ariel Rothner,Sarah Knapp,Alina Nemirovski,Danny Ben-Zvi,Joseph Tam

STAR protocols 3:101778 PubMed36313539

2022

A protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution using tissue clearing and click chemistry.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengyuan Pang,Li Ye

Cell 185:1793-1805.e17 PubMed35483372

2022

In situ identification of cellular drug targets in mammalian tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengyuan Pang,Michael A Schafroth,Daisuke Ogasawara,Yu Wang,Victoria Nudell,Neeraj K Lal,Dong Yang,Kristina Wang,Dylan M Herbst,Jacquelyn Ha,Carlos Guijas,Jacqueline L Blankman,Benjamin F Cravatt,Li Ye

Cells 11: PubMed35269478

2022

Inhibitory Neurotransmission Is Sex-Dependently Affected by Tat Expression in Transgenic Mice and Suppressed by the Fatty Acid Amide Hydrolase Enzyme Inhibitor PF3845 via Cannabinoid Type-1 Receptor Mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Changqing Xu,Barkha J Yadav-Samudrala,Callie Xu,Bhupendra Nath,Twisha Mistry,Wei Jiang,Micah J Niphakis,Benjamin F Cravatt,Somnath Mukhopadhyay,Aron H Lichtman,Bogna M Ignatowska-Jankowska,Sylvia Fitting

International journal of molecular sciences 22: PubMed34360541

2021

Anandamide Concentration-Dependently Modulates Toll-Like Receptor 3 Agonism or UVB-Induced Inflammatory Response of Human Corneal Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ágnes Angyal,Zsófia Pénzes,Shahrzad Alimohammadi,Dorottya Horváth,Lili Takács,György Vereb,Barbara Zsebik,Tamás Bíró,Kinga Fanni Tóth,Erika Lisztes,Balázs István Tóth,Attila Oláh,Attila Gábor Szöllősi

Frontiers in synaptic neuroscience 13:689518 PubMed34122037

2021

Targeting Endocannabinoid Signaling in the Lateral Habenula as an Intervention to Prevent Mental Illnesses Following Early Life Stress: A Perspective.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan D Shepard,Fereshteh S Nugent

Journal of developmental origins of health and disease 13:90-100 PubMed33650480

2021

Late effects of early weaning on food preference and the dopaminergic and endocannabinoid systems in male and female rats.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia Novaes Soares,Rosiane Aparecida Miranda,Iala Milene Bertasso,Carla Bruna Pietrobon,Vanessa Silva Tavares Rodrigues,Elaine de Oliveira,Alex Christian Manhães,Egberto Gaspar de Moura,Patricia Cristina Lisboa

Scientific reports 10:15556 PubMed32968163

2020

The fatty-acid amide hydrolase inhibitor URB597 inhibits MICA/B shedding.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuma Sekiba,Motoyuki Otsuka,Takahiro Seimiya,Eri Tanaka,Kazuyoshi Funato,Yu Miyakawa,Kazuhiko Koike
View all publications
style
left-column
style
left-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
style
left-column
style
right-column

関連製品

Select an associated product type
Alternative Product
Primary Antibodies

AB128917

Anti-FAAH1 antibody [EPR7549]

primary-antibodies

faah1-antibody-epr7549-ab128917

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com