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リコンビナントRabMAb

Anti-F4/80 抗体 [EPR26545-166] (BSA and Azide free) (ab300422)

Submit a review Submit a question References (3)
Western blot - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)
  • Western blot - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)
  • Western blot - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)
  • Immunohistochemistry (Frozen sections) - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)
  • Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (ab300422)
  • Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26545-166] to F4/80 - BSA and Azide free
  • Suitable for: IHC-Fr, IHC-P, WB, mIHC, ICC/IF
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free Unconjugated Unconjugated

製品の概要

  • 製品名

    Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free)
    F4/80 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26545-166] to F4/80 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-Fr, IHC-P, WB, mIHC, ICC/IFmore details
    適用なし: Flow Cyt

  • 種交差性

    交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: J774A.1 (mouse reticulum cell sarcoma macrophage), RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), Mouse spleen tissue. IHC: Mouse liver, mouse spleen, rat liver, rat spleen. IHC/FR: Mouse spleen (fresh). ICC/IF: RAW 264.7. mIHC: Mouse spleen, mouse bone marrow, mouse thymus, mouse lymph node

  • 特記事項

    ab300422 is a carrier free version of ab300421. 

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300422の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 97 kDa.
mIHC
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
特記事項
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 97 kDa.
mIHC
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt.

ターゲット情報

  • 機能

    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

  • 組織特異性

    Expression is restricted to eosinophils.

  • 配列類似性

    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ADGRE1 antibody
      • Adhesion G protein coupled receptor E1 antibody
      • Adhesion G protein-coupled receptor E1 antibody
      • AGRE1_HUMAN antibody
      • Cell surface glycoprotein EMR1 antibody
      • Cell surface glycoprotein F4/80 antibody
      • DD7A5 7 antibody
      • Egf like module containing mucin like hormone receptor like 1 antibody
      • Egf like module containing mucin like hormone receptor like sequence 1 antibody
      • EGF like module receptor 1 antibody
      • EGF TM7 antibody
      • EGF-like module receptor 1 antibody
      • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
      • EGFTM7 antibody
      • EMR 1 antibody
      • EMR1 antibody
      • EMR1 hormone receptor antibody
      • Gpf480 antibody
      • Ly71 antibody
      • Lymphocyte antigen 71 antibody
      • TM7LN3 antibody
      see all

    画像

    • Western blot - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)
      Western blot - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)
      All lanes : Anti-F4/80 antibody [EPR26545-166] (ab300421) at 1/1000 dilution

      Lane 1 : J774A.1 (mouse reticulum cell sarcoma macrophage) whole cell lysate
      Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 97 kDa
      Observed band size: 90-160 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      This data was developed using ab300421, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Negative control: NIH/3T3 (PMID:8550607).

      The antibody recognizes glycosylated form (120~160KDa) and unglycosylated form(~90KDa) of F4/80(PMID:10547361) in the unboiled samples.

    • Western blot - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)
      Western blot - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)
      All lanes : Anti-F4/80 antibody [EPR26545-166] (ab300421) at 1/1000 dilution

      Lane 1 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
      Lane 2 : L-929 ( mouse connective tissue fibroblast) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 97 kDa
      Observed band size: 160 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      This data was developed using ab300421, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Negative control: L-929 (PMID:8550607).

      The samples are unboiled.

    • Western blot - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)
      Western blot - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)
      Anti-F4/80 antibody [EPR26545-166] (ab300421) at 1/1000 dilution + Mouse spleen tissue lysate 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 97 kDa


      Exposure time: 26 seconds


      This data was developed using ab300421, the same antibody clone in a different buffer formulation.

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The samples are unboiled.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)

      This data was developed using ab300421, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of Paraffin-embedded sections of mouse liver tissue labelling F4/80 with ab300421 at 1/5000 dilution for 30min at room temperature, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the Kupffer cells of Mouse liver (PMID: 26473015) is observed. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: The secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)

      This data was developed using ab300421, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of Paraffin-embedded sections of mouse spleen tissue labelling F4/80 with ab300421 at 1/5000 dilution for 30min at room temperature, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the macrophages of mouse spleen (PMID: 19820169) is observed. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: The secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)

      This data was developed using ab300421, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of Paraffin-embedded sections of rat liver tissue labelling F4/80 with ab300421 at 1/5000 dilution for 30min at room temperature, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the Kupffer cells of rat liver is observed. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: The secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)

      This data was developed using ab300421, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of Paraffin-embedded sections of Rat spleen tissue labelling F4/80 with ab300421 at 1/5000 dilution for 30min at room temperature, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the macrophages of rat spleen is observed. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: The secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins.

    • Immunohistochemistry (Frozen sections) - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)
      Immunohistochemistry (Frozen sections) - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)

      This data was developed using ab300421, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of 0.2% Triton X-100 permeabilised, 4% PFA fixed of fresh Mouse spleen tissue labelling F4/80 with ab300421 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution. Positive staining on the macrophages of mouse spleen is observed.

      The nuclear countertain is DAPI.

    • Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (ab300422)
      Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (ab300422)

      This data was developed using ab300421, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% TritonX-100 permeabilised RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) labelling F4/80 with ab300421 at 1/50 dilution, followed Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 1/1000 dilution. Confocal image showing membranous and cytoplasmic staining in RAW264.7 cell line. Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594)(ab195889) was used to counterstain Microtubule at 1/200 dilution. The nuclear counterstain is DAPI.

      Negative control: NIH/3T3 (PMID: 8550607). 

    • Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)
      Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) (AB300422)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (3)

    ab300422 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300422 は 3 報の論文で使用されています。

    • Hichami A  et al. In Vitro Functional Characterization of Type-I Taste Bud Cells as Monocytes/Macrophages-like Which Secrete Proinflammatory Cytokines. Int J Mol Sci 24:N/A (2023). PubMed: 37373472
    • Dai Y  et al. A construct of adipose-derived mesenchymal stem cells-laden collagen scaffold for fertility restoration by inhibiting fibrosis in a rat model of endometrial injury. Regen Biomater 10:rbad080 (2023). PubMed: 37808957
    • Ding Z  et al. Nanosized Silk-Magnesium Complexes for Tissue Regeneration. Adv Healthc Mater 12:e2300887 (2023). PubMed: 37317936

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