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リコンビナントRabMAb

Anti-F4/80 抗体 [EPR26545-166] (ab300421)

Reviews (2) Submit a question References (7)
Western blot - Anti-F4/80 antibody [EPR26545-166] (AB300421)
  • Western blot - Anti-F4/80 antibody [EPR26545-166] (AB300421)
  • Western blot - Anti-F4/80 antibody [EPR26545-166] (AB300421)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)
  • Immunohistochemistry (Frozen sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)
  • Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [EPR26545-166] (AB300421)
  • Anti-F4/80 antibody [EPR26545-166] (AB300421)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR26545-166] to F4/80
  • Suitable for: mIHC, ICC/IF, IHC-P, IHC-Fr, WB
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free Carrier Free Unconjugated

製品の概要

  • 製品名

    Anti-F4/80 antibody [EPR26545-166]
    F4/80 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR26545-166] to F4/80

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: mIHC, ICC/IF, IHC-P, IHC-Fr, WBmore details
    適用なし: Flow Cyt

  • 種交差性

    交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: J774A.1 (mouse reticulum cell sarcoma macrophage), RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), Mouse spleen tissue. IHC: Mouse liver, mouse spleen, rat liver, rat spleen. IHC-Fr: Mouse spleen (fresh). ICC/IF: RAW 264.7. mIHC: Mouse spleen, mouse bone marrow, mouse thymus, mouse lymph node

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR26545-166

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300421の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
mIHC
1/5000.
ICC/IF
1/50.
IHC-P (2)
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/500.
WB
1/1000. Predicted molecular weight: 97 kDa.

Not suitable for Rat.
特記事項
mIHC
1/5000.
ICC/IF
1/50.
IHC-P
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/500.
WB
1/1000. Predicted molecular weight: 97 kDa.

Not suitable for Rat.
追加情報
Is unsuitable for Flow Cyt.

ターゲット情報

  • 機能

    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

  • 組織特異性

    Expression is restricted to eosinophils.

  • 配列類似性

    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ADGRE1 antibody
      • Adhesion G protein coupled receptor E1 antibody
      • Adhesion G protein-coupled receptor E1 antibody
      • AGRE1_HUMAN antibody
      • Cell surface glycoprotein EMR1 antibody
      • Cell surface glycoprotein F4/80 antibody
      • DD7A5 7 antibody
      • Egf like module containing mucin like hormone receptor like 1 antibody
      • Egf like module containing mucin like hormone receptor like sequence 1 antibody
      • EGF like module receptor 1 antibody
      • EGF TM7 antibody
      • EGF-like module receptor 1 antibody
      • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
      • EGFTM7 antibody
      • EMR 1 antibody
      • EMR1 antibody
      • EMR1 hormone receptor antibody
      • Gpf480 antibody
      • Ly71 antibody
      • Lymphocyte antigen 71 antibody
      • TM7LN3 antibody
      see all

    画像

    • Western blot - Anti-F4/80 antibody [EPR26545-166] (AB300421)
      Western blot - Anti-F4/80 antibody [EPR26545-166] (AB300421)
      Anti-F4/80 antibody [EPR26545-166] (ab300421) at 1/1000 dilution + Mouse spleen tissue lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 97 kDa
      Observed band size: 160 kDa why is the actual band size different from the predicted?


      Exposure time: 26 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      The samples are unboiled.

    • Western blot - Anti-F4/80 antibody [EPR26545-166] (AB300421)
      Western blot - Anti-F4/80 antibody [EPR26545-166] (AB300421)
      Lane 1 : Anti-F4/80 antibody [EPR26545-166] (ab300421) at 1/1000 dilution


      Lane 1 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
      Lane 2 : L-929 ( mouse connective tissue fibroblast) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 97 kDa
      Observed band size: 160 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Negative control: L-929 (PMID:8550607).

      The samples are unboiled.

    • Western blot - Anti-F4/80 antibody [EPR26545-166] (AB300421)
      Western blot - Anti-F4/80 antibody [EPR26545-166] (AB300421)
      All lanes : Anti-F4/80 antibody [EPR26545-166] (ab300421) at 1/1000 dilution

      Lane 1 : J774A.1 (mouse reticulum cell sarcoma macrophage) whole cell lysate
      Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 97 kDa
      Observed band size: 90-160 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

      Negative control: NIH/3T3 (PMID:8550607).

      The antibody recognizes glycosylated form (120~160KDa) and unglycosylated form(~90KDa) of F4/80(PMID:10547361) in the unboiled samples.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)

      Immunohistochemical analysis of Paraffin-embedded sections of Rat spleen tissue labelling F4/80 with ab300421 at 1/5000 dilution for 30min at room temperature, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the macrophages of rat spleen is observed. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: The secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)

      Immunohistochemical analysis of Paraffin-embedded sections of rat liver tissue labelling F4/80 with ab300421 at 1/5000 dilution for 30min at room temperature, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the Kupffer cells of rat liver is observed. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: The secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)

      Immunohistochemical analysis of Paraffin-embedded sections of mouse spleen tissue labelling F4/80 with ab300421 at 1/5000 dilution for 30min at room temperature, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the macrophages of mouse spleen (PMID: 19820169) is observed. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: The secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)

      Immunohistochemical analysis of Paraffin-embedded sections of mouse liver tissue labelling F4/80 with ab300421 at 1/5000 dilution for 30min at room temperature, followed by LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the Kupffer cells of Mouse liver (PMID: 26473015) is observed. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

      Counterstained with Hematoxylin.

      Secondary antibody only control: The secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution) for 20 mins.

    • Immunohistochemistry (Frozen sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)
      Immunohistochemistry (Frozen sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)

      Immunohistochemical analysis of 0.2% Triton X-100 permeabilised, 4% PFA fixed of fresh Mouse spleen tissue labelling F4/80 with ab300421 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution. Positive staining on the macrophages of mouse spleen is observed.

      The nuclear countertain is DAPI.

    • Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [EPR26545-166] (AB300421)
      Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [EPR26545-166] (AB300421)

      Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% TritonX-100 permeabilised RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) labelling F4/80 with ab300421 at 1/50 dilution, followed Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 1/1000 dilution. Confocal image showing membranous and cytoplasmic staining in RAW264.7 cell line. Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594)(ab195889) was used to counterstain Microtubule at 1/200 dilution. The nuclear counterstain is DAPI.

      Negative control: NIH/3T3 (PMID: 8550607)

    • Anti-F4/80 antibody [EPR26545-166] (AB300421)
      Anti-F4/80 antibody [EPR26545-166] (AB300421)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (7)

    ab300421 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300421 は 7 報の論文で使用されています。

    • Ouhaddi Y  et al. Development of Neovasculature in Axially Vascularized Calcium Phosphate Cement Scaffolds. J Funct Biomater 14:N/A (2023). PubMed: 36826904
    • Hu Q  et al. Reducing GEF-H1 Expression Inhibits Renal Cyst Formation, Inflammation, and Fibrosis via RhoA Signaling in Nephronophthisis. Int J Mol Sci 24:N/A (2023). PubMed: 36834937
    • Yang Z  et al. A nanotherapeutic strategy that engages cytotoxic and immunosuppressive activities for the treatment of cancer recurrence following organ transplantation. EBioMedicine 92:104594 (2023). PubMed: 37167784
    • Zhang Z  et al. [FARSB stratifies prognosis and cold tumor microenvironment across different cancer types: an integrated single cell and bulk RNA sequencing analysis]. Nan Fang Yi Ke Da Xue Xue Bao 43:667-679 (2023). PubMed: 37313807
    • Zhang S  et al. Plexin D1 mediates disturbed flow-induced M1 macrophage polarization in atherosclerosis. Heliyon 9:e17314 (2023). PubMed: 37389065
    • Yao Y  et al. PSTPIP2 regulates synovial macrophages polarization and dynamics via ERβ in the joint microenvironment. Arthritis Res Ther 24:247 (2022). PubMed: 36324152
    • Tang L  et al. Astragaloside IV Targets Macrophages to Alleviate Renal Ischemia-Reperfusion Injury via the Crosstalk between Hif-1α and NF-κB (p65)/Smad7 Pathways. J Pers Med 13:N/A (2022). PubMed: 36675720

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-F4/80 antibody [EPR26545-166]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Carl Hobbs

    Verified customer

    投稿 Aug 20 2023

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-F4/80 antibody [EPR26545-166]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (E12.5 Mouse Embryo)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6
    Permeabilization
    No
    Specification
    E12.5 Mouse Embryo
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Mary Green

    Verified customer

    投稿 Jan 20 2023

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