Anti-F4/80 抗体 - C-terminal (ab186073)
Key features and details
- Chicken polyclonal to F4/80 - C-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgY
製品の概要
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製品名
Anti-F4/80 antibody - C-terminal
F4/80 一次抗体 製品一覧 -
製品の詳細
Chicken polyclonal to F4/80 - C-terminal -
由来種
Chicken -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat, Human
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免疫原
Synthetic peptide corresponding to Mouse F4/80 (C terminal).
Database link: Q61549 -
ポジティブ･コントロール
- Mouse spleen lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 98% PBS, 1% Trehalose -
Concentration information loading... -
精製度
IgY fraction -
特記事項（精製）
Crude antiserum was purified by Thiopropyl Sepharose affinity chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgY -
研究分野
関連製品
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab186073の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 102 kDa.
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| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 102 kDa. |
ターゲット情報
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機能
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. -
組織特異性
Expression is restricted to eosinophils. -
配列類似性
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2015 Human
- Entrez Gene: 13733 Mouse
- Entrez Gene: 316137 Rat
- Omim: 600493 Human
- SwissProt: Q14246 Human
- SwissProt: Q61549 Mouse
- SwissProt: Q5Y4N8 Rat
- Unigene: 2375 Human
see all -
別名
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
see all
画像
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Western blot - Anti-F4/80 antibody - C-terminal (ab186073)Anti-F4/80 antibody - C-terminal (ab186073) at 1 µg/ml + Mouse spleen lysate
Developed using the ECL technique.
Predicted band size: 102 kDa
Exposure time: 5 seconds -
Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody - C-terminal (ab186073)Chicken anti mouse F4/80 (ab186073, polyclonal) staining of mouse RAW cells by immunofluorescence, using a FITC-conjugated anti chicken antibody as a secondary antibody.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (5)
ab186073 は 5 報の論文で使用されています。
- Hofstee MI et al. A murine Staphylococcus aureus fracture-related infection model characterised by fracture non-union, staphylococcal abscess communities and myeloid-derived suppressor cells. Eur Cell Mater 41:774-792 (2021). PubMed: 34151416
- Andreichenko IN et al. 4-methylumbelliferone Prevents Liver Fibrosis by Affecting Hyaluronan Deposition, FSTL1 Expression and Cell Localization. Int J Mol Sci 20:N/A (2019). PubMed: 31847129
- Amini-Nik S et al. Accumulation of myeloid lineage cells is mapping out liver fibrosis post injury: a targetable lesion using Ketanserin. Exp Mol Med 50:81 (2018). PubMed: 30026607
- Masola V et al. Heparanase regulates the M1 polarization of renal macrophages and their crosstalk with renal epithelial tubular cells after ischemia/reperfusion injury. FASEB J 32:742-756 (2018). PubMed: 28970256
- Secchi MF et al. Heparanase and macrophage interplay in the onset of liver fibrosis. Sci Rep 7:14956 (2017). PubMed: 29097791
