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リコンビナントRabMAb

Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) 抗体 [EP2122Y] (ab76247)

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Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
  • Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
  • Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
  • Dot Blot - Anti-Ezrin (phospho Thr567)/ Radixin (phospho Thr564)/ Moesin (phospho Thr558) antibody [EP2122Y] (ab76247)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2122Y] to Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558)
  • Suitable for: WB, IHC-P, Dot blot
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y]
    Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP2122Y] to Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558)

  • 由来種

    Rabbit

  • 特異性

    ab76247 will detect Ezrin, Radixin and Moesin. This antibody detects the phosphorylated target and not the non-phosphorylated epitope.

  • アプリケーション

    適用あり: WB, IHC-P, Dot blotmore details
    適用なし: Flow Cyt

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558). The exact sequence is proprietary. A synthetic peptide corresponding to residues surrounding Thr567 (Ezrin), Thr564 (Radixin) and Thr558 (Moesin) of human Ezrin.

  • ポジティブ･コントロール

    • WB: HeLa, HEK-293, NIH/3T3, and C6 starved overnight, then treated with 100nM Calyculin A whole cell lysates. IHC-P: Human breast, mouse testis, and rat liver tissues. Dot Blot: Ezrin (phospho Thr567)/ Radixin (phospho Thr564)/ Moesin (phospho Thr558) phospho peptide and Non-phospho peptide.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab76247の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 75-81 kDa (predicted molecular weight: 69 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Dot blot
1/1000.
特記事項
WB
1/1000. Detects a band of approximately 75-81 kDa (predicted molecular weight: 69 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Dot blot
1/1000.
追加情報
Is unsuitable for Flow Cyt.

画像

  • Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    All lanes : Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247) at 1/1000 dilution

    Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight, then treated with 100nM Calyculin A for 30 minutes whole cell lysate
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight, then treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
    Lane 4 : Untreated HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 5 : HEK-293 (Human embryonic kidney epithelial cell) starved overnight, then treated with 100nM Calyculin A for 60 minutes whole cell lysate
    Lane 6 : HEK-293 (Human embryonic kidney epithelial cell) starved overnight, then treated with 100nM Calyculin A for g0 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 69 kDa
    Observed band size: 75-81 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)

    Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) with ab76247 at 1/500 (1.64 µg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Membranous staining on human breast.

    The section was incubated with ab76247 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Counterstained with Hematoxylin.

  • Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    All lanes : Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247) at 1/1000 dilution

    Lane 1 : Untreated NIH/3T3 (Mouse embyonic fibroblast) whole cell lysate
    Lane 2 : Untreated NIH/3T3 (Mouse embyonic fibroblast) treated with 100nM Calyculin A for 30 minutes whole cell lysate
    Lane 3 : Untreated NIH/3T3 (Mouse embyonic fibroblast) treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 69 kDa
    Observed band size: 75-81 kDa why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) with ab76247 at 1/500 (1.64 µg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Membranous staining on human breast.

    The section was incubated with ab76247 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Counterstained with Hematoxylin.

  • Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    All lanes : Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247) at 1/1000 dilution

    Lane 1 : Untreated C6 (Rat glial tumor glial cell) whole cell lysate
    Lane 2 : Untreated C6 (Rat glial tumor glial cell) treated with 100ng/ml Calyculin A for 60 minutes whole cell lysate
    Lane 3 : Untreated C6 (Rat glial tumor glial cell) treated with 100ng/ml Calyculin A for 60 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 69 kDa
    Observed band size: 75-81 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) with ab76247 at 1/500 (1.64 µg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Membranous staining on human breast.

    The section was incubated with ab76247 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Counterstained with Hematoxylin.

  • Dot Blot - Anti-Ezrin (phospho Thr567)/ Radixin (phospho Thr564)/ Moesin (phospho Thr558) antibody [EP2122Y] (ab76247)
    Dot Blot - Anti-Ezrin (phospho Thr567)/ Radixin (phospho Thr564)/ Moesin (phospho Thr558) antibody [EP2122Y] (ab76247)

    Dot blot analysis of Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) phospho peptide (Lane 1), Non-phospho peptide (Lane 2), labelling Ezrin (phospho Thr567)/ Radixin (phospho Thr564)/ Moesin (phospho Thr558) with ab76247 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The sequence of non-phospho peptide: CGRDKYKTLRQIR.
    The sequence of phospho peptide: CGRDKYK-pT-LRQIR.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (11)

ab76247 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab76247 は 11 報の論文で使用されています。

  • Liu J  et al. Inhibition of TRPV4 remodels single cell polarity and suppresses the metastasis of hepatocellular carcinoma. Cell Death Dis 14:379 (2023). PubMed: 37369706
  • Wang X  et al. Formative pluripotent stem cells show features of epiblast cells poised for gastrulation. Cell Res 31:526-541 (2021). PubMed: 33608671
  • Zhang L  et al. STK10 knockout inhibits cell migration and promotes cell proliferation via modulating the activity of ERM and p38 MAPK in prostate cancer cells. Exp Ther Med 22:851 (2021). PubMed: 34149897
  • Li Y  et al. CircGLIS3 Promotes High-Grade Glioma Invasion via Modulating Ezrin Phosphorylation. Front Cell Dev Biol 9:663207 (2021). PubMed: 34540823
  • Zhang L  et al. Knockout of STK10 promotes the migration and invasion of cervical cancer cells. Transl Cancer Res 9:7079-7090 (2020). PubMed: 35117313
View all Publications for this product

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1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa Cells)
Permeabilization
Yes - 0.3% Triton
Specification
HeLa Cells
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 19 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y]

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.3% Triton X-100
Specification
HeLa
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 09 2018

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