Anti-Ezrin (phospho Y478) 抗体 (ab79256)
Key features and details
- Rabbit polyclonal to Ezrin (phospho Y478)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Ezrin (phospho Y478) antibody
Ezrin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Ezrin (phospho Y478) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human Ezrin (phospho Y478).
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ポジティブ･コントロール
- Extracts form HeLa cells treated with Na3VO4 (0.3nM, 40mins).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab79256の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 69 kDa.
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| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 69 kDa. |
ターゲット情報
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機能
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. -
組織特異性
Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied. -
配列類似性
Contains 1 FERM domain. -
発生段階
Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain. -
翻訳後修飾
Phosphorylated by tyrosine-protein kinases. -
細胞内局在
Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein. - Information by UniProt
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参照データベース
- Entrez Gene: 7430 Human
- Entrez Gene: 22350 Mouse
- Entrez Gene: 54319 Rat
- Omim: 123900 Human
- SwissProt: P15311 Human
- SwissProt: P26040 Mouse
- SwissProt: P31977 Rat
- Unigene: 487027 Human
see all -
別名
- Villin 2 ezrin antibody
- CVIL antibody
- CVL antibody
see all
画像
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Western blot - Anti-Ezrin (phospho Y478) antibody (ab79256)All lanes : Anti-Ezrin (phospho Y478) antibody (ab79256) at 1/500 dilution
Lane 1 : K562 cell lysate treated with Na3VO4 (0.3nM,
40mins)
Lane 2 : K562 cell lysate treated with Na3VO4 (0.3nM,
40mins) with immunizing phosphopeptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 69 kDa
Observed band size: 69 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab79256 は 1 報の論文で使用されています。
- Liu P et al. Ezrin/NF-?B Pathway Regulates EGF-induced Epithelial-Mesenchymal Transition (EMT), Metastasis, and Progression of Osteosarcoma. Med Sci Monit 24:2098-2108 (2018). WB . PubMed: 29628496