Anti-Ezrin (phospho Y353) 抗体 [EP925Y] (ab52916)
![Western blot - Anti-Ezrin (phospho Y353) antibody [EP925Y] (ab52916)](https://www.abcam.co.jp/ps/products/52/ab52916/Images/ab52916wb.jpg "Western blot - Anti-Ezrin (phospho Y353) antibody [EP925Y] (ab52916)")
Key features and details
- Rabbit monoclonal [EP925Y] to Ezrin (phospho Y353)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
製品の概要
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製品名
Anti-Ezrin (phospho Y353) antibody [EP925Y]
Ezrin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP925Y] to Ezrin (phospho Y353) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Lysate of 293 cells treated with pervanadate.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP925Y -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52916の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/200000. Predicted molecular weight: 69 kDa.
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| 特記事項 |
|---|
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WB
1/200000. Predicted molecular weight: 69 kDa. |
ターゲット情報
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機能
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. -
組織特異性
Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied. -
配列類似性
Contains 1 FERM domain. -
発生段階
Very strong staining is detected in the Purkinje cell layer and in part of the molecular layer of the infant brain compared to adult brain. -
翻訳後修飾
Phosphorylated by tyrosine-protein kinases. -
細胞内局在
Apical cell membrane. Cell projection. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes (By similarity). Microvillar peripheral membrane protein. - Information by UniProt
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参照データベース
- Entrez Gene: 7430 Human
- Omim: 123900 Human
- SwissProt: P15311 Human
- Unigene: 487027 Human
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別名
- Villin 2 ezrin antibody
- CVIL antibody
- CVL antibody
see all
画像
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Western blot - Anti-Ezrin (phospho Y353) antibody [EP925Y] (ab52916)All lanes : Anti-Ezrin (phospho Y353) antibody [EP925Y] (ab52916) at 1/200000 dilution
Lane 1 : 293 cell lysate untreated
Lane 2 : 293 cell lysate treated with pervanadate
Lysates/proteins at 10 µg per lane.
Predicted band size: 69 kDa
Observed band size: 69 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab52916 は 1 報の論文で使用されています。
- Liu P et al. Ezrin/NF-?B Pathway Regulates EGF-induced Epithelial-Mesenchymal Transition (EMT), Metastasis, and Progression of Osteosarcoma. Med Sci Monit 24:2098-2108 (2018). WB . PubMed: 29628496