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AB75840

Anti-Ezrin 抗体 [EP924Y]

Anti-Ezrin antibody [EP924Y]

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(6 Publications)

Rabbit Recombinant Monoclonal Ezrin antibody. Suitable for WB and reacts with Human, Rat, Mouse samples. Cited in 6 publications.

別名を表示する

VIL2, EZR, Ezrin, Cytovillin, Villin-2, p81

1 Images
Western blot - Anti-Ezrin antibody [EP924Y] (AB75840)
  • WB

Lab

Western blot - Anti-Ezrin antibody [EP924Y] (AB75840)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time :
Lane 1 : 20 seconds;
Lane 2 and Lane 3 : 180 seconds.

All lanes:

Western blot - Anti-Ezrin antibody [EP924Y] (ab75840) at 1/2000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

Lane 2:

Mouse brain lysate at 15 µg

Lane 3:

Rat brain lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 69 kDa

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関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-Ezrin antibody [EP924Y] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP924Y

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

<p>ab75840 detects both phosphorylated and non phosphorylated Ezrin.</p>

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代替製品

Primary Antibodies

AB79256

Anti-Ezrin (phospho Y478) antibody

Western blot - Anti-Ezrin (phospho Y478) antibody (AB79256)

  • 0
  • 0
Primary Antibodies

AB47293

Anti-Ezrin (phospho T567) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (phospho T567) antibody (AB47293)

  • 5
  • 3
Primary Antibodies

AB205381

Anti-Ezrin antibody [6F1A9]

Flow Cytometry - Anti-Ezrin antibody [6F1A9] (AB205381)

  • 0
  • 0
Primary Antibodies

AB4069

Anti-Ezrin antibody [3C12]

BOND RX™ Validated|KO Validated

Flow Cytometry (Intracellular) - Anti-Ezrin antibody [3C12] (AB4069)

  • 3
  • 8
Primary Antibodies

AB235927

Anti-Ezrin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Ezrin antibody (AB235927)

  • 0
  • 0
Primary Antibodies

AB198522

HRP Anti-Ezrin antibody [EP886Y]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Ezrin antibody [EP886Y] (AB198522)

  • 0
  • 0
Primary Antibodies

AB133297

Anti-Ezrin antibody [EPR803]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EPR803] (AB133297)

  • 0
  • 0
Primary Antibodies

AB40839

Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker

20ul selling size|RabMAb|Recombinant|KO Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (AB40839)

  • 5
  • 5
Primary Antibodies

AB52916

Anti-Ezrin (phospho Y353) antibody [EP925Y]

20ul selling size|RabMAb

Western blot - Anti-Ezrin (phospho Y353) antibody [EP925Y] (AB52916)

  • 0
  • 0
Primary Antibodies

AB198520

Alexa Fluor® 488 Anti-Ezrin antibody [EP886Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Ezrin antibody [EP886Y] (AB198520)

  • 0
  • 0
Primary Antibodies

AB204954

Anti-Ezrin antibody [6F1C3]

Flow Cytometry - Anti-Ezrin antibody [6F1C3] (AB204954)

  • 0
  • 0
Primary Antibodies

AB239832

Anti-Ezrin antibody [EP886Y] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - BSA and Azide free (AB239832)

  • 0
  • 0

組合せ製品

Assay Kits

AB278116

Human Ezrin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Ezrin ELISA Kit (AB278116)

  • 0
  • 0
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB76247

Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (AB76247)

  • 5
  • 2
Primary Antibodies

AB52490

Anti-Moesin antibody [EP1863Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Moesin antibody [EP1863Y] (AB52490)

  • 5
  • 5
Primary Antibodies

AB52495

Anti-Radixin/RDX antibody [EP1862Y]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Radixin/RDX antibody [EP1862Y] (AB52495)

  • 5
  • 4
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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製品の詳細

Production method for this product has been changed from hybridoma to recombinant on 15th August 2024

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Conditional Ambient
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Ezrin also known as cytovillin or villin-2 is a protein belonging to the ezrin-radixin-moesin (ERM) family. It is approximately 81 kDa in molecular weight. Ezrin plays a critical role in linking the actin cytoskeleton to the plasma membrane. This interaction occurs through its ability to bind to membrane proteins and phospholipids facilitating structural support and signal transduction. Ezrin is widely expressed in epithelial tissues but it also occurs in other cell types such as lymphocytes and osteoclasts.
Biological function summary

Ezrin functions as a membrane-microfilament linker and is essential for maintaining cell shape adhesion and motility. It is an integral component of many cellular structures including microvilli and ruffles. Ezrin’s activity often involves forming complexes with other proteins such as EBP50 and F-actin which are fundamental for its role in cellular morphogenesis. These interactions allow ezrin to regulate membrane surface structure and cell surface organization.

Pathways

Ezrin participates in various signaling pathways that are essential for cell migration and invasion. One key pathway is the Rho family of GTPases which regulates actin cytoskeleton dynamics. Ezrin interacts with proteins like Rho-associated kinase (ROCK) that influence cell contraction and motility. Additionally ezrin is involved in the MAPK/ERK pathway which influences cell growth and survival by interacting with other signaling molecules.

Ezrin shows significant relevance to cancer progression and metastasis particularly in conditions like osteosarcoma. Ezrin overexpression correlates with increased metastatic potential and poor clinical outcomes. It also has implications in allergic diseases due to its role in immune cell function. In these contexts ezrin interacts with SRC family kinases which are important for transmitting signals from cell-surface receptors to downstream pathways influencing disease development and progression.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
See full target information EZR
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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39125907

2024

Proteomic Analysis Reveals Physiological Activities of Aβ Peptide for Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaorui Ai,Zeyu Cao,Zhaoru Ma,Qinghuan Liu,Wei Huang,Taolei Sun,Jing Li,Chenxi Yang

Experimental and therapeutic medicine 21:112 PubMed33335575

2020

Increased expression of IFN-γ in preeclampsia impairs human trophoblast invasion via a SOCS1/JAK/STAT1 feedback loop.

Applications

Unspecified application

Species

Unspecified reactive species

Huiqiang Liu,Wenhao Wang,Chongdong Liu

Experimental and therapeutic medicine 17:2495-2502 PubMed30906437

2019

Role of mTOR signaling in the regulation of high glucose-induced podocyte injury.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuyue Li,Yan Zeng,Qing Jiang,Cong Wu,Jing Zhou

Translational oncology 11:1-10 PubMed29132012

2017

Distinct Ezrin Truncations Differentiate Metastases in Sentinel Lymph Nodes from Unaffected Lymph Node Tissues, from Primary Breast Tumors, and from Healthy Glandular Breast Tissues.

Applications

WB

Species

Human

Claudia Röwer,Christian George,Toralf Reimer,Bernd Stengel,Anngret Radtke,Bernd Gerber,Michael O Glocker

Reproductive biology and endocrinology : RB&E 7:95 PubMed19737422

2009

Participation of ezrin in bacterial uptake by trophoblast giant cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kenta Watanabe,Masato Tachibana,Suk Kim,Masahisa Watarai

FEBS letters 582:3663-8 PubMed18840437

2008

MicroRNA-183 regulates Ezrin expression in lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Guofu Wang,Weimin Mao,Shu Zheng
View all publications
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