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AB205381

Anti-Ezrin 抗体 [6F1A9]

Anti-Ezrin antibody [6F1A9]

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(2 Publications)

Mouse Monoclonal Ezrin antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, African green monkey, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EZR aa 250-500.

別名を表示する

VIL2, EZR, Ezrin, Cytovillin, Villin-2, p81

6 Images
Flow Cytometry - Anti-Ezrin antibody [6F1A9] (AB205381)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Ezrin antibody [6F1A9] (AB205381)

Flow cytometric analysis of HeLa cells labeling Ezrin using ab205381 at 1/200 dilution (green) and negative control (red).

Immunocytochemistry/ Immunofluorescence - Anti-Ezrin antibody [6F1A9] (AB205381)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ezrin antibody [6F1A9] (AB205381)

Immunofluorescent analysis of A549 cells labeling Ezrin using ab205381 at 1/100 dilution (green). Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Flow Cytometry - Anti-Ezrin antibody [6F1A9] (AB205381)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Ezrin antibody [6F1A9] (AB205381)

Flow cytometric analysis of MCF7 cells labeling Ezrin using ab205381 at 1/200 dilution (green) and negative control (red).

Western blot - Anti-Ezrin antibody [6F1A9] (AB205381)
  • WB

Supplier Data

Western blot - Anti-Ezrin antibody [6F1A9] (AB205381)

Expected MW is 47.1 kDa

All lanes:

Western blot - Anti-Ezrin antibody [6F1A9] (ab205381) at 1/500 dilution

All lanes:

Human Ezrin (AA: 292-464) recombinant protein.

Predicted band size: 69 kDa

false

Western blot - Anti-Ezrin antibody [6F1A9] (AB205381)
  • WB

Supplier Data

Western blot - Anti-Ezrin antibody [6F1A9] (AB205381)

All lanes:

Western blot - Anti-Ezrin antibody [6F1A9] (ab205381) at 1/500 dilution

Lane 1:

Non-transfected HEK293 cell lysate

Lane 2:

Ezrin (AA: 292-464)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 69 kDa

false

Western blot - Anti-Ezrin antibody [6F1A9] (AB205381)
  • WB

Supplier Data

Western blot - Anti-Ezrin antibody [6F1A9] (AB205381)

All lanes:

Western blot - Anti-Ezrin antibody [6F1A9] (ab205381) at 1/500 dilution

Lane 1:

MCF7 cell lysate

Lane 2:

HeLa cell lysate

Lane 3:

A431 cell lysate

Lane 4:

HEK293 cell lysate

Lane 5:

SK N SH cell lysate

Lane 6:

Jurkat cell lysate

Lane 7:

HepG2 cell lysate

Lane 8:

NIH 3T3 cell lysate

Lane 9:

COS-7 cell lysate

Predicted band size: 69 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

6F1A9

アイソタイプ

IgG1

キャリアフリー

No

交差種

Mouse, Human, African green monkey

アプリケーション

WB, Flow Cyt, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human EZR aa 250-500. The exact immunogen used to generate this antibody is proprietary information.

P15311

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代替製品

Primary Antibodies

AB4069

Anti-Ezrin antibody [3C12]

BOND RX™ Validated|KO Validated

Flow Cytometry (Intracellular) - Anti-Ezrin antibody [3C12] (AB4069)

  • 3
  • 8
Primary Antibodies

AB198522

HRP Anti-Ezrin antibody [EP886Y]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Ezrin antibody [EP886Y] (AB198522)

  • 0
  • 0
Primary Antibodies

AB133297

Anti-Ezrin antibody [EPR803]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EPR803] (AB133297)

  • 0
  • 0
Primary Antibodies

AB40839

Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker

20ul selling size|RabMAb|Recombinant|KO Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (AB40839)

  • 5
  • 5
Primary Antibodies

AB198520

Alexa Fluor® 488 Anti-Ezrin antibody [EP886Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Ezrin antibody [EP886Y] (AB198520)

  • 0
  • 0
Primary Antibodies

AB75840

Anti-Ezrin antibody [EP924Y]

20ul selling size|RabMAb|KO Validated|Recombinant

Western blot - Anti-Ezrin antibody [EP924Y] (AB75840)

  • 0
  • 0
Primary Antibodies

AB239832

Anti-Ezrin antibody [EP886Y] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - BSA and Azide free (AB239832)

  • 0
  • 0

組合せ製品

Assay Kits

AB278116

Human Ezrin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Ezrin ELISA Kit (AB278116)

  • 0
  • 0
Primary Antibodies

AB52495

Anti-Radixin/RDX antibody [EP1862Y]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Radixin/RDX antibody [EP1862Y] (AB52495)

  • 5
  • 4
Primary Antibodies

AB52490

Anti-Moesin antibody [EP1863Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Moesin antibody [EP1863Y] (AB52490)

  • 5
  • 5
Primary Antibodies

AB76247

Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (AB76247)

  • 5
  • 2
Primary Antibodies

AB308329

Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Control - BSA & Azide free

Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Sodium Potassium ATPase alpha 1 antibody [HL114] - Plasma Membrane Control - BSA & Azide free (AB308329)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/400", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/400", "ICCIF-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/400", "ICCIF-species-notes": "<p></p>" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein G
精製に関する特記事項
Purified from tissue culture supernatant.
バッファー組成
Preservative: 0.05% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Ezrin also known as cytovillin or villin-2 is a protein belonging to the ezrin-radixin-moesin (ERM) family. It is approximately 81 kDa in molecular weight. Ezrin plays a critical role in linking the actin cytoskeleton to the plasma membrane. This interaction occurs through its ability to bind to membrane proteins and phospholipids facilitating structural support and signal transduction. Ezrin is widely expressed in epithelial tissues but it also occurs in other cell types such as lymphocytes and osteoclasts.
Biological function summary

Ezrin functions as a membrane-microfilament linker and is essential for maintaining cell shape adhesion and motility. It is an integral component of many cellular structures including microvilli and ruffles. Ezrin’s activity often involves forming complexes with other proteins such as EBP50 and F-actin which are fundamental for its role in cellular morphogenesis. These interactions allow ezrin to regulate membrane surface structure and cell surface organization.

Pathways

Ezrin participates in various signaling pathways that are essential for cell migration and invasion. One key pathway is the Rho family of GTPases which regulates actin cytoskeleton dynamics. Ezrin interacts with proteins like Rho-associated kinase (ROCK) that influence cell contraction and motility. Additionally ezrin is involved in the MAPK/ERK pathway which influences cell growth and survival by interacting with other signaling molecules.

Ezrin shows significant relevance to cancer progression and metastasis particularly in conditions like osteosarcoma. Ezrin overexpression correlates with increased metastatic potential and poor clinical outcomes. It also has implications in allergic diseases due to its role in immune cell function. In these contexts ezrin interacts with SRC family kinases which are important for transmitting signals from cell-surface receptors to downstream pathways influencing disease development and progression.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
See full target information EZR
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 301:110418 PubMed40570961

2025

Functional investigation of the RNA helicase MOV10 with respect to its interplay with factors involved in nonsense-mediated mRNA decay.

Applications

Unspecified application

Species

Unspecified reactive species

Guangpu Xue,Gabriel P Faber,Lea S Pommerening,Megha Mallick,Aditi Gupta,Markus C Wahl,Yaron Shav-Tal,Sutapa Chakrabarti

Journal of cellular biochemistry 120:14885-14898 PubMed31044454

2019

Upregulation of microRNA-140-3p inhibits epithelial-mesenchymal transition, invasion, and metastasis of hepatocellular carcinoma through inactivation of the MAPK signaling pathway by targeting GRN.

Applications

Unspecified application

Species

Unspecified reactive species

Qiu-Yin Zhang,Chang-Jun Men,Xue-Wei Ding
View all publications
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