AB176115

Anti-EZH1 抗体 - N-terminal

Name: Anti-EZH1 抗体 - N-terminal (ab176115) | Abcam
Brand: Abcam Limited
SKU: AB176115
Price: 75000 JPY
Availability: InStock
Rating: 4 (1 reviews)

Anti-EZH1 antibody - N-terminal

(1 Review)

(5 Publications)

Rabbit Polyclonal EZH1 antibody. N-terminal. Suitable for WB and reacts with Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human EZH1 aa 150-250.

別名を表示する

KIAA0388, EZH1, Histone-lysine N-methyltransferase EZH1, ENX-2, Enhancer of zeste homolog 1

1 Images

Supplier Data

Western blot - Anti-EZH1 antibody - N-terminal (AB176115)

Lane 1:

Western blot - Anti-EZH1 antibody - N-terminal (ab176115) at 1 µg/mL

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Western blot - Anti-EZH1 antibody - N-terminal (ab176115) at 2 µg/mL

All lanes:

mouse lung tissue lysate at 15 µg

Predicted band size: 85 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse

アプリケーション

applications

免疫原

Synthetic Peptide within Human EZH1 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

Q92800

特異性

Multiple isoforms of EZH1 are known to exist. EZH1 ab176115 is predicted to not cross-react with EZH2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

精製に関する特記事項

ab176115 is affinity chromatography purified via peptide column.

バッファー組成

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

EZH1 also known as Enhancer of Zeste Homolog 1 functions as a histone-lysine N-methyltransferase. This enzyme plays a role in transcriptional repression through histone methylation specifically the trimethylation of histone H3 at lysine 27 (H3K27me3). The mass of EZH1 is approximately 85 kDa. Expression of EZH1 spans various tissues with notable presence in embryonic stem cells and adult tissues.

Biological function summary

The protein EZH1 participates in the formation of the Polycomb Repressive Complex 2 (PRC2). Within this complex EZH1 collaborates with other proteins to regulate gene expression epigenetically. PRC2 activity influences cell identity and development by maintaining the silenced state of specific genes. The presence of EZH1 in this complex indicates its significance in modulating developmental pathways and cellular differentiation processes.

Pathways

EZH1 contributes to epigenetic regulation and is involved in the Wnt signaling pathway. This enzyme's action influences cell cycle regulation and stem cell pluripotency. Within these pathways EZH1 is functionally related to proteins like EZH2 which shares a similar role in histone methylation. The interaction of EZH1 with these pathways highlights its influence on maintaining genetic expression patterns through cell division and differentiation.

EZH1 shows a connection to cancer and developmental disorders. Alterations in EZH1 expression or function can contribute to the development of certain cancers including prostate cancer through its interaction with EZH2. Additionally dysregulation of EZH1-related epigenetic pathways can lead to developmental disorders such as Weaver syndrome. This links EZH1 to critical regulatory roles in both normal development and disease pathology.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.

See full target information EZH1

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 18:4513-4531 PubMed35864958

2022

Maternal EHMT2 is essential for homologous chromosome segregation by regulating transcription in oocyte meiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Tie-Gang Meng,Wen-Long Lei,Xukun Lu,Xiao-Yu Liu,Xue-Shan Ma,Xiao-Qing Nie,Zheng-Hui Zhao,Qian-Nan Li,Lin Huang,Yi Hou,Ying-Chun Ouyang,Lei Li,Tie-Shan Tang,Heide Schatten,Wei Xie,Shao-Rong Gao,Xiang-Hong Ou,Zhen-Bo Wang,Qing-Yuan Sun

ACS pharmacology & translational science 5:491-507 PubMed35837138

2022

Targeting Triple-Negative Breast Cancer by a Novel Proteolysis Targeting Chimera Degrader of Enhancer of Zeste Homolog 2.

Applications

Unspecified application

Species

Unspecified reactive species

Brandon Dale,Chris Anderson,Kwang-Su Park,H Ümit Kaniskan,Anqi Ma,Yudao Shen,Chengwei Zhang,Ling Xie,Xian Chen,Xufen Yu,Jian Jin

Nature communications 11:6354 PubMed33311485

2020

PRC2 and EHMT1 regulate H3K27me2 and H3K27me3 establishment across the zygote genome.

Applications

Unspecified application

Species

Unspecified reactive species

Tie-Gang Meng,Qian Zhou,Xue-Shan Ma,Xiao-Yu Liu,Qing-Ren Meng,Xian-Ju Huang,Hong-Lin Liu,Wen-Long Lei,Zheng-Hui Zhao,Ying-Chun Ouyang,Yi Hou,Heide Schatten,Xiang-Hong Ou,Zhen-Bo Wang,Shao-Rong Gao,Qing-Yuan Sun

Cell stem cell 24:769-784.e6 PubMed30905620

2019

Mesenchymal Niche-Specific Expression of Cxcl12 Controls Quiescence of Treatment-Resistant Leukemia Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Puneet Agarwal,Stephan Isringhausen,Hui Li,Andrew J Paterson,Jianbo He,Álvaro Gomariz,Takashi Nagasawa,César Nombela-Arrieta,Ravi Bhatia

Nature neuroscience 16:1745-53 PubMed24162653

2013

EZH2-mediated H3K27 trimethylation mediates neurodegeneration in ataxia-telangiectasia.

Applications

Unspecified application

Species

Unspecified reactive species

Jiali Li,Ronald P Hart,Elyse M Mallimo,Mavis R Swerdel,Alexander W Kusnecov,Karl Herrup

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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For licensing inquiries, please contact partnerships@abcam.com

Anti-EZH1 抗体 - N-terminal

Anti-EZH1 antibody - N-terminal

Western blot - Anti-EZH1 antibody - N-terminal (AB176115)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (5)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise