Anti-EXOC2 抗体 [EPR9420]

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-EXOC2 antibody [EPR9420] (AB140620)

Intracellular flow cytometric analysis of permeabilized BxPC-3 cells labelling EXOC2 with ab140620 at 1/10 dilution (red) or a Rabbit IgG negative control (green).

• WB

Lab

Western blot - Anti-EXOC2 antibody [EPR9420] (AB140620)

Lanes 1- 4 : Merged signal (red and green). Green - ab140620 observed at 104 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab140620 was shown to react with EXOC2 in wild-type HeLa cells in western blot. The band observed in CRISPR/Cas9 edited cell line ab265839 (CRISPR/Cas9 edited cell lysate ab257944) lane below 104kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and EXOC2 CRISPR/Cas9 edited HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab140620 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-EXOC2 antibody [EPR9420] (ab140620) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

EXOC2 CRISPR/Cas9 edited HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human EXOC2 knockout HeLa cell line (<a href='/products/cell-lines/human-exoc2-knockout-hela-cell-line-ab265839'>ab265839</a>)

Lane 3:

SH-SY5Y cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Predicted band size: 104 kDa

Observed band size: 104 kDa

false

• WB

Lab

Western blot - Anti-EXOC2 antibody [EPR9420] (AB140620)

Lanes 1- 4 : Merged signal (red and green). Green - ab140620 observed at 104 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab140620 was shown to react with EXOC2 in wild-type HeLa cells in western blot. The band observed in knockout cell line ab265839 (knockout cell lysate ab257944) lane below 104kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and EXOC2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab140620 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-EXOC2 antibody [EPR9420] (ab140620) at 1000 µg

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

EXOC2 knockout HeLa cell lysate at 20 µg

Lane 3:

SH-SY5Y cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Predicted band size: 104 kDa

Observed band size: 104 kDa

false

• WB

Unknown

Western blot - Anti-EXOC2 antibody [EPR9420] (AB140620)

All lanes:

Western blot - Anti-EXOC2 antibody [EPR9420] (ab140620) at 1/1000 dilution

Lane 1:

BxPC-3 cell lysate at 10 µg

Lane 2:

Human placenta tissue lysate at 10 µg

Lane 3:

Fetal brain tissue lysate at 10 µg

Lane 4:

SH-SY5Y cell lysate at 10 µg

Predicted band size: 104 kDa

false