Anti-ETFA 抗体 (ab153722)
Key features and details
- Rabbit polyclonal to ETFA
- Suitable for: ICC/IF, WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ETFA antibody
ETFA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ETFA -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-P, IPmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 87-329 of Human ETFA (Uniprot ID: P13804).
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ポジティブ･コントロール
- WB: A431, HeLa, HEK-293T, HepG2, C8D30 and PC12 whole cell lysates; ICC/IF: HeLa cells. IHC-P: Human MCF7 xenograft tissue; Mouse brain stem, kidney, urinary bladder and cervix tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab153722の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/100 - 1/1000.
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| WB |
1/500 - 1/3000. Predicted molecular weight: 35 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
1/100 - 1/500.
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| 特記事項 |
|---|
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ICC/IF
1/100 - 1/1000. |
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WB
1/500 - 1/3000. Predicted molecular weight: 35 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
1/100 - 1/500. |
ターゲット情報
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機能
The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). -
関連疾患
Glutaric aciduria 2A -
配列類似性
Belongs to the ETF alpha-subunit/FixB family. -
ドメイン
Domain I shares an identical polypeptide fold with the beta subunit ETFB though there is no sequence similarity. -
翻訳後修飾
The N-terminus is blocked. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 2108 Human
- Entrez Gene: 110842 Mouse
- Entrez Gene: 300726 Rat
- Omim: 608053 Human
- SwissProt: P13804 Human
- SwissProt: Q99LC5 Mouse
- SwissProt: P13803 Rat
- Unigene: 39925 Human
see all -
別名
- Alpha ETF antibody
- Alpha-ETF antibody
- Electron transfer flavoprotein alpha polypeptide antibody
see all
画像
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Western blot - Anti-ETFA antibody (ab153722)All lanes : Anti-ETFA antibody (ab153722) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa -
Immunoprecipitation - Anti-ETFA antibody (ab153722)ETFA was immunoprecipitated from HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract with 5 µg ab153722. Western blot was performed from the immunoprecipitate using ab153722.
Lane 1: IgG instead of ab153722 in HepG2 whole cell lysate.
Lane 2: ab153722 IP in HepG2 whole cell lysate.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFA antibody (ab153722)Paraffin-embedded mouse brain stem tissue stained for ETFA using ab153722 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFA antibody (ab153722)Paraffin-embedded mouse kidney tissue stained for ETFA using ab153722 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFA antibody (ab153722)Paraffin-embedded mouse urinary bladder tissue stained for ETFA using ab153722 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFA antibody (ab153722)Paraffin-embedded mouse cervix tissue stained for ETFA using ab153722 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-ETFA antibody (ab153722)Anti-ETFA antibody (ab153722) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 35 kDa
10% SDS PAGE -
Western blot - Anti-ETFA antibody (ab153722)Anti-ETFA antibody (ab153722) + C8D30 whole cell lysate at 30 µg
Predicted band size: 35 kDa
10 % SDS-PAGE -
Western blot - Anti-ETFA antibody (ab153722)Anti-ETFA antibody (ab153722) at 1/1000 dilution + PC12 whole cell lysate at 30 µg
Predicted band size: 35 kDa
10 % SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-ETFA antibody (ab153722)Immunofluorescent analysis of methanol-fixed HeLa cells labeling ETFA with ab153722 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ETFA antibody (ab153722)Immunohistochemical analysis of paraffin-embedded Human MCF7 xenograft tissue labeling ETFA with ab153722 at 1/250 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab153722 は 1 報の論文で使用されています。
- Yu HM et al. Repression of let-7b-5p prevents the development of multifidus muscle dysfunction by promoting vitamin D accumulation via upregulation of electron transfer flavoprotein alpha subunit in a rat model of multifidus muscle injury. J Cell Biochem 120:7458-7473 (2019). PubMed: 30387180