Anti-Estrogen Related Receptor alpha 抗体 (ab137489)
Key features and details
- Rabbit polyclonal to Estrogen Related Receptor alpha
- Suitable for: ChIP, IHC-P, IP, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Estrogen Related Receptor alpha antibody
Estrogen Related Receptor alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Estrogen Related Receptor alpha -
由来種
Rabbit -
アプリケーション
適用あり: ChIP, IHC-P, IP, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow, Dog, Pig
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免疫原
Synthetic peptide corresponding to Human Estrogen Related Receptor alpha aa 1-45 (N terminal).
Sequence:MSSQVVGIEPLYIKAEPASPDSPKGSSETETEPPVALAPGPAPTR
Database link: P11474 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab137489の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ChIP |
Use at an assay dependent concentration.
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| IHC-P |
1/100 - 1/1000.
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| IP |
1/100 - 1/500.
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| WB |
1/500 - 1/3000. Predicted molecular weight: 46 kDa.
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| 特記事項 |
|---|
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ChIP
Use at an assay dependent concentration. |
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IHC-P
1/100 - 1/1000. |
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IP
1/100 - 1/500. |
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WB
1/500 - 1/3000. Predicted molecular weight: 46 kDa. |
ターゲット情報
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機能
Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism. -
配列類似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
翻訳後修飾
Phosphorylation on Ser-19 enhances sumoylation on Lys-14 increasing repression of transcriptional activity.
Sumoylated by SUMO2. Main site is Lys-14 which is enhanced by phosphorylation on Ser-19, cofactor activation, and by interaction with PIAS4. Sumoylation enhances repression of transcriptional activiy, but has no effect on subcellular location nor on DNA binding. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 507834 Cow
- Entrez Gene: 403169 Dog
- Entrez Gene: 2101 Human
- Entrez Gene: 26379 Mouse
- Entrez Gene: 100322868 Pig
- Entrez Gene: 293701 Rat
- Omim: 601998 Human
- SwissProt: Q6QMY5 Dog
see all -
別名
- Err 1 antibody
- ERR a antibody
- ERR alpha antibody
see all
画像
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Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489)Anti-Estrogen Related Receptor alpha antibody (ab137489) at 1/500 dilution + Neuro2A whole cell extract at 30 µg
Predicted band size: 46 kDa10% SDS-PAGE
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Western blot - Anti-Estrogen Related Receptor alpha antibody (ab137489)Anti-Estrogen Related Receptor alpha antibody (ab137489) at 1/500 dilution + Rat2 whole cell extract at 30 µg
Predicted band size: 46 kDa10% SDS-PAGE
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ChIP - Anti-Estrogen Related Receptor alpha antibody (ab137489)Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-ERR alpha antibody. The precipitated DNA was detected by PCR with primer set targeting to PS2 promoter or GAPDH promoter.
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Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody (ab137489)ERR alpha was immunoprecipitated from 40µ of HEK-293T whole cell lysate with ab137489 at 1/1000 dilution. Secondary antibody EasyBlot anti-rabbit IgG was used as secondary antibody.
Lane 1: 40 μg HEK-293T whole cell lysate.
Lane 2: Control with 2 μg of preimmune rabbit IgG.
Lane 3: Immunoprecipitation of ERR alpha protein by 2 μg of ERR alpha antibody.7.5% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Related Receptor alpha antibody (ab137489)Paraffin embedded mouse heart tissue stained for ERR alpha using ab137489 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-Estrogen Related Receptor alpha antibody - N-terminal (ab137489)All lanes : Anti-Estrogen Related Receptor alpha antibody (ab137489) at 1/500 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 46 kDa
12% SDS PAGE
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab137489 は 4 報の論文で使用されています。
- Ruan GY et al. An integrated approach of network pharmacology, molecular docking, and experimental verification uncovers kaempferol as the effective modulator of HSD17B1 for treatment of endometrial cancer. J Transl Med 21:204 (2023). PubMed: 36932403
- Tang S et al. ERRα Up-Regulates Invadopodia Formation by Targeting HMGCS1 to Promote Endometrial Cancer Invasion and Metastasis. Int J Mol Sci 24:N/A (2023). PubMed: 36835419
- Su P et al. ERRα promotes glycolytic metabolism and targets the NLRP3/caspase-1/GSDMD pathway to regulate pyroptosis in endometrial cancer. J Exp Clin Cancer Res 42:274 (2023). PubMed: 37864196
- Mao X et al. Lipid reprogramming induced by the TFEB-ERRα axis enhanced membrane fluidity to promote EC progression. J Exp Clin Cancer Res 41:28 (2022). PubMed: 35045880