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AB237995

Anti-Estrogen Receptor 抗体 [ESR1/1935]

Anti-Estrogen Receptor antibody [ESR1/1935]

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(1 Publication)

Mouse Monoclonal Estrogen Receptor alpha antibody. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ESR1.

別名を表示する

ESR, NR3A1, ESR1, Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor antibody [ESR1/1935] (AB237995)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor antibody [ESR1/1935] (AB237995)

Formalin-fixed, paraffin-embedded human Endometrial Carcinoma tissue stained for Estrogen Receptor using ab237995 at 2 μg/mL in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor antibody [ESR1/1935] (AB237995)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor antibody [ESR1/1935] (AB237995)

Formalin-fixed, paraffin-embedded human Breast Carcinoma tissue stained for Estrogen Receptor using ab237995 at 2 μg/mL in immunohistochemical analysis.

Western blot - Anti-Estrogen Receptor antibody [ESR1/1935] (AB237995)
  • WB

Supplier Data

Western blot - Anti-Estrogen Receptor antibody [ESR1/1935] (AB237995)

All lanes:

Western blot - Anti-Estrogen Receptor antibody [ESR1/1935] (ab237995) at 1 µg/mL

All lanes:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Predicted band size: 66 kDa

false

Protein Array - Anti-Estrogen Receptor antibody [ESR1/1935] (AB237995)
  • Protein Array

Unknown

Protein Array - Anti-Estrogen Receptor antibody [ESR1/1935] (AB237995)

ab237995 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

ESR1/1935

アイソタイプ

IgG2a

軽鎖のタイプ

kappa

キャリアフリー

No

交差種

Human

アプリケーション

IHC-P, WB, Protein Array

applications

免疫原

Recombinant Full Length Protein corresponding to Human ESR1.

P03372

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Primary incubation for 30 minutes at room temperature.</p><p>Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.</p>" }, "Recombinant full length protein - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A/G
精製に関する特記事項
Purified from Bioreactor concentrate.
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The Estrogen Receptor (ER) also known by its alternate names NR3A1 (nuclear receptor subfamily 3 group A member 1) is a nuclear hormone receptor that binds estrogens such as the 17 beta estradiol molecule. Its molecular mass is approximately 66 kDa. This receptor locates mainly in the cytoplasm and nucleus of cells within tissues such as the breast uterus and bone. The ER exists in two primary subtypes ER alpha and ER beta which differ in their tissue distribution and functional roles.
Biological function summary

The Estrogen Receptor functions as a transcription factor that regulates gene expression when it binds to its ligand which is commonly estradiol. Upon binding 17 beta estradiol the receptor dimerizes and interacts with specific DNA sequences to influence transcription. It significantly regulates genes responsible for cell growth differentiation and reproductive functions. The estradiol chemical structure allows for specific interactions with ER altering the transcriptional regulation in a ligand-dependent manner. Though functioning mainly as a monomer ER can form heterodimers with other nuclear receptors affecting its biological role.

Pathways

The Estrogen Receptor is an important player in the genomic signaling pathway where it modulates the expression of numerous target genes by direct DNA binding. It also participates in the non-genomic signaling pathway where it involves the activation of second messengers and kinases. ER closely associates with important proteins such as coactivators and corepressors in these pathways. Estradiol chemical formula interactions facilitate molecular events involving ER-alpha to influence components of cell proliferation and survival.

The Estrogen Receptor is intricately linked to breast cancer and osteoporosis. Dysfunction in ER signaling promotes the proliferation of breast cancer cells often requiring targeted therapies that inhibit its action. In osteoporosis ER activity impacts bone density and turnover through regulation of osteoclasts and osteoblasts. The receptor's interaction with growth factor receptors like HER2 in breast cancer further highlights its significance in disease pathogenesis.

製品プロトコール

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ターゲットの情報

The protein expressed by the gene ESR1 functions as a nuclear hormone receptor involved in regulating eukaryotic gene expression, affecting cellular proliferation and differentiation in target tissues. It mediates ligand-dependent nuclear transactivation either through direct homodimer binding to estrogen response elements (ERE) or by associating with other transcription factors like AP-1/c-Jun, c-Fos, ATF-2, Sp1, and Sp3, allowing ERE-independent signaling. Ligand binding triggers a conformational change, facilitating the interaction with coactivator complexes through LXXLL motifs. This interaction leads to mutual transrepression with NF-kappa-B in a cell-type-specific manner, reducing NF-kappa-B DNA-binding activity and transcription from the IL6 promoter while displacing coregulators like RELA/p65. It can antagonistically or synergistically interact with NF-kappa-B for transcription activation, involving recruitment to adjacent response elements, working with CREBBP. Additionally, it activates the transcription of TFF1 and mediates membrane-initiated signaling through kinase cascades. It's crucial for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 specifically activates NOS3 and endothelial nitric oxide production, while isoforms lacking certain functional domains modulate transcriptional activity by competitive ligand or DNA binding and heterodimerization with the full-length receptor, capable of binding to ERE and inhibiting isoform 1. This supplementary information is collated from multiple sources and compiled automatically.
See full target information ESR1

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of respiratory and critical care medicine 206:260-270 PubMed35550018

2022

Characterization of Immunopathology and Small Airway Remodeling in Constrictive Bronchiolitis.

Applications

Unspecified application

Species

Unspecified reactive species

Sergey S Gutor,Bradley W Richmond,Rui-Hong Du,Pingsheng Wu,Jae Woo Lee,Lorraine B Ware,Ciara M Shaver,Sergey V Novitskiy,Joyce E Johnson,John H Newman,Stephen I Rennard,Robert F Miller,Timothy S Blackwell,Vasiliy V Polosukhin
View all publications

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