Name: Anti-Estrogen Receptor alpha antibody [EPR703(2)]
Brand: RabMAb
SKU: ab79413
Rating: 4 (7 reviews)

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Western blot - Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR703(2)] to Estrogen Receptor alpha
Suitable for: WB, IHC-P
Reacts with: Human

Carrier Free

製品名

Anti-Estrogen Receptor alpha antibody [EPR703(2)]
Estrogen Receptor alpha 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR703(2)] to Estrogen Receptor alpha
由来種

Rabbit
特異性

Expression levels of ER alpha protein vary with sample type.
アプリケーション

適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IP
種交差性

交差種: Human
交差が予測される動物種: Baboon
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- MCF-7 cell lysate. Human breast carcinoma tissue.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
精製度

Tissue culture supernatant
ポリ/モノ

モノクローナル
クローン名

EPR703(2)
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-Estrogen Receptor alpha antibody [EPR703(2)] - BSA and Azide free (ab214455)
Assay kits
- Estrogen Receptor Transcription Factor Assay Kit (Colorimetric) (ab207203)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- MCF7 nuclear extract lysate (ab14860)
Recombinant Protein
- Recombinant Human Estrogen Receptor alpha protein (ab82606)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab79413の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB		1/500 - 1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 68 kDa).
IHC-P	(7)	1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

特記事項
WB 1/500 - 1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 68 kDa).
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

追加情報

Is unsuitable for Flow Cyt,ICC/IF or IP.

機能

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
配列類似性

Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
ドメイン

Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
翻訳後修飾

Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3).
細胞内局在

Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
Target information above from: UniProt accession P03372 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 2099 Human
- Omim: 133430 Human
- SwissProt: P03372 Human
- Unigene: 208124 Human
別名
- 7*/654 isoform antibody
- 7*/819 2 isoform antibody
- 7*/822 isoform antibody
- 8*/901 isoform antibody
- 8*/941 isoform antibody
- DKFZp686N23123 antibody
- ER alpha antibody
- ER antibody
- ER-alpha antibody
- Era antibody
- ESR antibody
- ESR1 antibody
- ESR1_HUMAN antibody
- ESRA antibody
- Estradiol receptor antibody
- Estrogen nuclear receptor alpha antibody
- Estrogen receptor 1 antibody
- Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
- Estrogen receptor alpha antibody
- Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
- Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
- Estrogen receptor alpha delta 4 +49 isoform antibody
- Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
- Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
- Estrogen receptor alpha E1 E2 1 2 antibody
- Estrogen receptor alpha E1 N2 E2 1 2 antibody
- Estrogen receptor antibody
- ESTRR antibody
- NR3A1 antibody
- Nuclear receptor subfamily 3 group A member 1 antibody
see all

Western blot - Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)

All lanes : Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413) at 1/2000 dilution

Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell). Whole cell lysates
Lane 2 : T-47D (human mammary gland ductal carcinoma epithelial cell). Whole cell lysates
Lane 3 : MDA-MB231 (Human breast adenocarcinoma epithelial cell) Whole cell lysates (Negative control)
Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) Whole cell lysates (Negative control)
Lane 5 : Human uterus whole tissue lysate
Lane 6 : Human ovary whole tissue lysate
Lane 7 : Human ovary cancer whole tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 68 kDa
Observed band size: 68 kDa

Exposure time: 3 minutes

Blocking and diluting buffer: 5% NFDM/TBST
Western blot - Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)

Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413) at 1/1000 dilution + MCF-7 cell lysate at 10 µg

Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 68 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)

ab79413, at 1/500 dilution, staining Estrogen Receptor alpha in paraffin-embedded human breast carcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)This image is courtesy of an Abreview submitted by Marcia Burch

ab79413 staining Estrogen Receptor alpha in Baboon placenta and fetal ovary tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% serum for 30 minutes at 22°C; antigen retrieval was by heat mediation in 10mM sodium citrate, pH 6.0. Samples were incubated with primary antibody (1/500 in PBS + 5% normal goat serum) for 16 hours at 4°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (7µg/ml) was used as the secondary antibody.
See Abreview
Anti-Estrogen Receptor alpha antibody [EPR703(2)] (ab79413)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab79413 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab79413 は 6 報の論文で使用されています。

Zou X et al. Characterization of Estrogen Receptors in Pancreatic Adenocarcinoma with Tertiary Lymphoid Structures. Cancers (Basel) 15:N/A (2023). PubMed: 36765788
Xin L et al. An Acellular Scaffold Facilitates Endometrial Regeneration and Fertility Restoration via Recruiting Endogenous Mesenchymal Stem Cells. Adv Healthc Mater 11:e2201680 (2022). PubMed: 36049781
Han N et al. The G-Protein-Coupled Estrogen Receptor (GPER) Regulates Trimethylation of Histone H3 at Lysine 4 and Represses Migration and Proliferation of Ovarian Cancer Cells In Vitro. Cells 10:N/A (2021). PubMed: 33799631
Liu Y et al. Efficacy and safety of TE/TEC/intensive paclitaxel neoadjuvant chemotherapy for the treatment of breast cancer. Oncol Lett 17:907-912 (2019). PubMed: 30655846
Xin L et al. A collagen scaffold loaded with human umbilical cord-derived mesenchymal stem cells facilitates endometrial regeneration and restores fertility. Acta Biomater 92:160-171 (2019). PubMed: 31075515
Hirao-Suzuki M et al. Repeated Exposure to 4-Methyl-2,4-bis(4-hydroxyphenyl)pent-1-ene (MBP), an Active Metabolite of Bisphenol A, Aggressively Stimulates Breast Cancer Cell Growth in an Estrogen Receptor ß (ERß)-Dependent Manner. Mol Pharmacol 95:260-268 (2019). PubMed: 30552153

レビューと Q&A

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レビューを送る質問を送る

1-9 of 9 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Ovary Carcinoma)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: pH 9.0 EDTA

Permeabilization

Specification

Ovary Carcinoma

Blocking step

PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 23 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Dog Tissue sections (placenta)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0

Permeabilization

Specification

placenta

Blocking step

Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 27 2016

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (placenta)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0

Permeabilization

Specification

placenta

Blocking step

Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 27 2016

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Cow Tissue sections (oviduct)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0

Permeabilization

Specification

oviduct

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 15 2015

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (placenta)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0

Permeabilization

Specification

placenta

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 13 2015

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Horse Tissue sections (vagina)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0

Permeabilization

Specification

vagina

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 06 2015

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate, pH 6.0

Sample

Baboon Tissue sections (placenta and fetal ovary)

Specification

placenta and fetal ovary

Permeabilization

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MS. Marcia Burch

Verified customer

投稿 Aug 12 2014

Question

Phone call enquiring if there are any primary antibodies available against Anti-Estrogen Receptor region 302-522. Had identified ab45005 (immunogen in region of 275-325) and ab14020 (fusion protein with residues 255-367 used as immunogen).

Abcam community

Verified customer

Asked on Nov 21 2011

Answer

thank you for contacting us. I am currently looking into wether we have any suitable antibodies for the specifications you have provided. We have a number of antibodies for which the immunogen used is within the C-terminal region of human Estrogen Receptor alpha (ab21232, ab15284. ab56177, ab1001499, ab79413 and ab45005 as you already have identified). However, in order to make sure that the immunogen used for each of these antibodies lies within your region of interest (residues 302-522) I am currently checking with the sources of these antibodies. I will also look into wether the two phospho specific antibodies we have (ab39222 and ab61049) may be suitable for your application. As soon as I have further information I will let you know.

Abcam Scientific Support

Answered on Nov 21 2011

Question

Dear Sir/Madam, I would like to introduce a human mutant form of ERα (S554fs, which carries a frameshift mutation in aa 554) in an ERa-positive mouse cell line. It is very important for my experimental design to have a human-specific antibody, that would not cross-react with the endogenous, mouse wild type ERα in Western blots. Failure to do so, would result in false positive results. Are the epitopes of your human-specific ERa antibody, catalogue number ab79413, upstream aa 554? I would greatly appreciate your response to my query at your earliest convenience, so that I can proceed to the purchase of the most suitable ERα antibody. Yours faithfully

Abcam community

Verified customer

Asked on Oct 15 2010

Answer

Thank you for your enquiry. I can confirm that the immunogen for ab79413 Estrogen Receptor alpha antibody [EPR703(2)] is not upstream of amino acid residue 554. It is specific to the C-terminus, ending at amino acid 594. Regrettably, I would suggest this antibody would not be suitable for your experiments, since it would not recognize the mutant ERα. Testing indicates that ER alpha will not cross-react with the endogenous, mouse wild type ERα in Western blot. I am sorry this is not better news for you on this occasion. If you have any further questions, please do not hesitate to contact us.

Abcam Scientific Support

Answered on Oct 15 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

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製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Assay kits

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

ドメイン

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (6)

レビューと Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor alpha antibody [EPR703(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor alpha antibody [EPR703(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor alpha antibody [EPR703(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor alpha antibody [EPR703(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor alpha antibody [EPR703(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor alpha antibody [EPR703(2)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor alpha antibody [EPR703(2)]