Anti-Estrogen Receptor alpha 抗体 (ab75635)
Key features and details
- Rabbit polyclonal to Estrogen Receptor alpha
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Estrogen Receptor alpha antibody
Estrogen Receptor alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Estrogen Receptor alpha -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human, capybara -
免疫原
Synthetic peptide corresponding to Human Estrogen Receptor alpha (N terminal).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75635の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
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| IP |
Use at 10 µg/mg of lysate.
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| IHC-P | (4) |
Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
Use a concentration of 2.5 - 5 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
|
IP
Use at 10 µg/mg of lysate. |
|
IHC-P
Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
ターゲット情報
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機能
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1. -
配列類似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
ドメイン
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
翻訳後修飾
Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3). -
細胞内局在
Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation. - Information by UniProt
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参照データベース
- Entrez Gene: 2099 Human
- Omim: 133430 Human
- SwissProt: P03372 Human
- Unigene: 208124 Human
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別名
- 7*/654 isoform antibody
- 7*/819 2 isoform antibody
- 7*/822 isoform antibody
see all
画像
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Western blot - Anti-Estrogen Receptor alpha antibody (ab75635)Anti-Estrogen Receptor alpha antibody (ab75635) at 5 µg/ml + MCF7 (Human breast adenocarcinoma cell line) cell lysate
Predicted band size: 68 kDa
Observed band size: 68 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (ab75635)ab75635 at 20 µg/ml dilution staining Estrogen Receptor alpha in breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and AEC chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (ab75635)This image is courtesy of an Abreview submitted by Raísa Brito.Immunohistochemical analysis of capybara testis tissue, labeling Estrogen receptor alpha with ab75635, diluted 1/1000. Heat mediated antigen retrieval with citrate buffer pH 6, blocked with BSA.
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Immunoprecipitation - Anti-Estrogen Receptor alpha antibody (ab75635)Staining of Estrogen Receptor alpha in MCF7 (Human breast adenocarcinoma cell line cell lysate immunoprecipitated using ab75635 at 10 µg/mg lysate.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (49)
ab75635 は 49 報の論文で使用されています。
- Bouck EG et al. High risk oral contraceptive hormones do not directly enhance endothelial cell procoagulant activity in vitro. PLoS One 18:e0284333 (2023). PubMed: 37075041
- Klein-Rodewald T et al. New C3H KitN824K/WT cancer mouse model develops late-onset malignant mammary tumors with high penetrance. Sci Rep 12:19793 (2022). PubMed: 36396684
- Escandon P et al. The Role of Estriol and Estrone in Keratoconic Stromal Sex Hormone Receptors. Int J Mol Sci 23:N/A (2022). PubMed: 35055103
- Fan Q et al. miR-130a-3p promotes cell proliferation and invasion by targeting estrogen receptor a and androgen receptor in cervical cancer. Exp Ther Med 21:414 (2021). PubMed: 33747155
- Gustafsson KL et al. Arginine site 264 in murine estrogen receptor-α is dispensable for the regulation of the skeleton. Am J Physiol Endocrinol Metab 320:E160-E168 (2021). PubMed: 33225718