Anti-ESRP2 抗体 (ab113486)
Key features and details
- Rabbit polyclonal to ESRP2
- Suitable for: IHC-P, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ESRP2 antibody
ESRP2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ESRP2 -
由来種
Rabbit -
特異性
ab113486 is predicted to not cross-react with other ESRP family members. At least two isoforms of ESRP2 are known to exist; ab113486 will detect both.
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アプリケーション
適用あり: IHC-P, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide containing a 17 residue sequence from the C terminal region of Human ESRP2 (GenBank accession no. NP_079215).
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ポジティブ･コントロール
- WB: Human lung tissue lysate. IHC: Human lung tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113486の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 20 µg/ml.
Immunohistochemistry (Immunoflurescence detection). |
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| WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 78 kDa.
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| IHC-P |
Use a concentration of 5 µg/ml.
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| 特記事項 |
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IHC-P
Use a concentration of 20 µg/ml. Immunohistochemistry (Immunoflurescence detection). |
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 78 kDa. |
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IHC-P
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2. -
組織特異性
Epithelial cell-specific. -
配列類似性
Belongs to the ESRP family.
Contains 3 RRM (RNA recognition motif) domains. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 80004 Human
- Omim: 612960 Human
- SwissProt: Q9H6T0 Human
- Unigene: 436585 Human
- Unigene: 592053 Human
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別名
- Epithelial splicing regulatory protein 2 antibody
- ESRP 2 antibody
- ESRP2 antibody
see all
画像
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Western blot - Anti-ESRP2 antibody (ab113486)Anti-ESRP2 antibody (ab113486) at 1 µg/ml + Human lung tissue lysate at 15 µg
Predicted band size: 78 kDa -
Immunohistochemistry paraffin embedded sections - Anti-ESRP2 antibody (ab113486)Immunohistochemistry (Immunofluorescence) of ESRP2 in Human lung tissue cells with ab113486 at 20 ug/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ESRP2 antibody (ab113486)Immunohistochemical analysis of Human lung tissue, staining ESRP2 with ab113486 at 5 µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab113486 は 6 報の論文で使用されています。
- Elhasnaoui J et al. A Regulatory Axis between Epithelial Splicing Regulatory Proteins and Estrogen Receptor α Modulates the Alternative Transcriptome of Luminal Breast Cancer. Int J Mol Sci 23:N/A (2022). PubMed: 35887187
- Becker T et al. Genetic information from discordant sibling pairs points to ESRP2 as a candidate trans-acting regulator of the CF modifier gene SCNN1B. Sci Rep 10:22447 (2020). PubMed: 33384439
- Munkley J et al. Androgen-regulated transcription of ESRP2 drives alternative splicing patterns in prostate cancer. Elife 8:N/A (2019). PubMed: 31478829
- Jeong HM et al. ESRP1 is overexpressed in ovarian cancer and promotes switching from mesenchymal to epithelial phenotype in ovarian cancer cells. Oncogenesis 6:e389 (2017). PubMed: 28991261
- Deloria AJ et al. Epithelial splicing regulatory protein 1 and 2 paralogues correlate with splice signatures and favorable outcome in human colorectal cancer. Oncotarget 7:73800-73816 (2016). Human . PubMed: 27650542
- Ishii H et al. Epithelial splicing regulatory proteins 1 (ESRP1) and 2 (ESRP2) suppress cancer cell motility via different mechanisms. J Biol Chem 289:27386-99 (2014). PubMed: 25143390