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Anti-ESAT6 抗体 (ab45073)

Reviews (3)Q&A (4)References (4)

Key features and details

  • Rabbit polyclonal to ESAT6
  • Suitable for: WB
  • Reacts with: Mycobacterium bovis
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-ESAT6 antibody

  • 製品の詳細

    Rabbit polyclonal to ESAT6

  • 由来種

    Rabbit

  • 特異性

    The rabbit polyclonal antibody to EsaT-6 recognizes the EsaT-6 (Rv3875) protein of Mycobacterium tuberculosis and Mycobacterium bovis.

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mycobacterium bovis, Mycobacterium tuberculosis

  • 免疫原

    Recombinant full length protein corresponding to ESAT6.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • バッファー

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab45073の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (2)
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 11 kDa.

Use under reducing conditions.
特記事項
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 11 kDa.

Use under reducing conditions.

ターゲット情報

  • 関連性

    The ESAT6 antigen from Mycobacterium tuberculosis is a dominant target for cell mediated immunity in the early phase of tuberculosis (TB) in TB patients as well as in various animal models. ESAT6 is not found in M. bovis BCG. Its function is not known but it elicits high level of IFN-gamma from memory effector cells during the first phase of a protective immune response.

  • 細胞内局在

    Secreted

  • 別名

    • 6 kDa early secretory antigenic target antibody
    • 6 kDa early secretory antigenic target ESXA antibody
    • ESAT 6 antibody
    • esaT6 antibody
    • ESXA antibody
    see all

参考文献 (4)

ab45073 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab45073 は 4 報の論文で使用されています。

  • Yang H  et al. Interception of host fatty acid metabolism by mycobacteria under hypoxia to suppress anti-TB immunity. Cell Discov 7:90 (2021). PubMed: 34608123
  • Sepulveda D  et al. Bioelectrochemical Detection of Mycobacterium tuberculosis ESAT-6 in an Antibody-Based Biomicrosystem. Sensors (Basel) 17:N/A (2017). PubMed: 28937645
  • Tan K  et al. Comparison of BCG prime-DNA booster and rBCG regimens for protection against tuberculosis. Hum Vaccin Immunother 10:391-8 (2014). PubMed: 24192709
  • Nguyen YH  et al. Rapid identification and drug susceptibility screening of ESAT-6 secreting Mycobacteria by a NanoELIwell assay. Sci Rep 2:635 (2012). Functional Studies . PubMed: 22957139

レビューと Q&A

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レビューを送る 質問を送る

1-7 of 7 Abreviews or Q&A

Western blot abreview for Anti-ESAT6 antibody

 Poor
Abreviews
Application
Western blot
Loading amount
0.5 µg
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Sample
Mycobacterium tuberculosis Recombinant protein (Cell lysate, supernatant)
Specification
Cell lysate, supernatant
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 18 2014

Immunoprecipitation abreview for Anti-ESAT6 antibody

 Poor
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Mycobacterium tuberculosis Cell lysate - other (M. tuberculosis culture filtrate)
Total protein in input
1000 µg
Specification
M. tuberculosis culture filtrate
Immuno-precipitation step
Protein G
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 11 2012

Western blot abreview for Anti-ESAT6 antibody

 Poor
Abreviews
abreview image
Application
Western blot
Sample
Mycobacterium tuberculosis Purified protein (M.tuberculosis culture filtrate)
Loading amount
15 µg
Specification
M.tuberculosis culture filtrate
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 02 2012

Question


Our customer forwareded this email to me so, I send information.

Order Number.***
Please arrange a credit note for our customer.

Thank you in advance.

Read More
Answer

Thank you for contacting us.
Your credit note ID is ***.
I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:
(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.
Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.
To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.
The credit note ID is for your reference only. Please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.
Please let me know if you have any questions or if there is anything else that we can do for you.

Read More

Question

Thank you foryour sincere response on myreview of ab45073.I am sorry toinformyouof negative results with this antibody.

We want to get a refund or credit (Abpoints??) on this antibody . Because we already purchasedanother antibody (ab26246) andused that for detecting specific proteins.

Please let meknowthe following refund process in detail.If you have any questions, please feel free tocontact us.

Read More
Answer

Thank you very much for your reply.

I can certainly arrange a credit or refund for you for this purchase. A credit can be held on your account and used against a future order, or we can issue a full refund. Do you have any information about your order, such as the order number or PO number? If this was ordered through a distributor, could you tell me which company you ordered throughand the date of the purchase?

I look forward to hearing from you. Please let me know if you have further questions, and have a nice day.

Read More

Question

AbID: 45073, Anti-ESAT6 antibody Rating: Poor Image: Anti-ESAT6 antibody for Western blot (Mycobacterium tuberculosis) Sample: Species: Mycobacterium tuberculosis Type: Purified protein Loading amount: 15 µg Specification: M.tuberculosis culture filtrate Application: Application: Gel Running Conditions: Reduced Denaturing (15% SDS-PAGE) Blocking step: Milk as blocking agent for 1 hour Blocking Concentration: 5% Other detail: Dilution: 1/4000 Incubation time: 16 hours at 4°C Diluent: 0.05% TBS-T Secondary Antibody: Name: Abcam antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) Conjugation: Dilution: 1/10000 Detection: Detection method: ECL Western Blotting Substrate Exposure: 1 minute and 0 seconds Bands: Specific: ?? kDa Non-specific: above 23 kDa Additional Data:

Read More
Answer

Thank you for submitting your recent review of ab45073. I am sorry to see that the results with this antibody were so poor.

We do fully guarantee this antibody to work in Western blot with M tuberculosis samples, so if you would like, I can send you a different antibody as a replacement for this product. I can alternatively arrange a credit or refund if you would prefer. If you'd like to accept one of these options, please let me know your original order information and I will set this up for you.

I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

Read More

Question

Hi Tech,

I have have a few question below:

He wanted to try the below antibodies

i. CFP10

ii. ESAT-6

iii. Ag85B

However I also have few enquiries;

1) All of these antibodies are compatible to full length antigen, but in my case I'm using epitopes. Does that means I can expect the same functionality of these antibodies towards my epitopes?

2) Do abcam provides antibody synthesis service because I have 2 additional epitopes that I am having trouble in finding primary antibody.

Thanks

Read More
Answer

Thank you for your interest in Abcam.

1) Indeed, I can confirm that all relevant antibodies (CFP10: ab45074, ab64753, ab64754; ESAT6: ab26246, ab45073; Ag85B: ab43019) have been generated against the full-length proteins. The exact binding sequences of these have unfortunately not been determined, except for ab26246 (residues EQQWNFAGIEAAA at positions 3-15). So if you are working with protein fragments (or epitopes), we cannot guarantee that these antibodies will work for you, as the binding sites might not be present and therefore the antibody might not bind. I could ask the lab whether the epitopes have been mapped and whether the antibodies would bind your fragments, if you could provide some details to your peptides.

2) We continually add antibodies and other protein detection products to our catalogue but we do not offer custom-made antibodies. However, we are happy to pass on the contact details of the following companies that do offer this service:

United States

Pocono Rabbit Farm and Laboratory (PRFL)
PO Box 240
Canadensis, PA 18325
Tel: 800-622-6381 - Toll Free in the United Statesand Canada
Tel: 570-595-7108 - Worldwide
Fax: 570-595-9277
Website: www.prfal.com

Maine Biotechnology Services, Inc.
1037R Forest Ave.
Portland Maine 04103
Tel: 800-925-9476 or 207-797-5454
Fax: 207-797-5595
Website: www.mainebiotechnology.com

Cocalico Biologicals, Inc.
PO Box 265
449 Stevens Road
Reamstown, PA 17567-0265
Tel: 717-336-1990
Fax: 717-336-1993
Website: www.cocalicobiologicals.com

United Kingdom

Charles River Laboratories International, Inc.
Tel: +44 (0) 1843 823575
Website: www.criver.com

I hope this information is helpful to you. Please do not hesitate to contact us if you have any questions.

Read More

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