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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-ERp57 抗体 [EPR10678(B)] (ab154191)

Submit a review Submit a question References (2)
Western blot - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
  • Western blot - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
  • Anti-ERp57 antibody [EPR10678(B)] (ab154191)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10678(B)] to ERp57
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-ERp57 antibody [EPR10678(B)]
    ERp57 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR10678(B)] to ERp57

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • HepG2 cell lysate, 293T cell lysate, HeLa cell lysate, paraffin-embedded Human pancreas tissue

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR10678(B)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab154191の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 組織特異性

    Detected in the flagellum and head region of spermatozoa (at protein level).

  • 配列類似性

    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.

  • 細胞内局在

    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 58 kDa glucose regulated protein antibody
      • 58 kDa glucose-regulated protein antibody
      • 58 kDa microsomal protein antibody
      • Disulfide isomerase ER 60 antibody
      • Disulfide isomerase ER-60 antibody
      • Endoplasmic reticulum resident protein 57 antibody
      • Endoplasmic reticulum resident protein 60 antibody
      • ER p57 antibody
      • ER protein 57 antibody
      • ER protein 60 antibody
      • ERp 57 antibody
      • ERp57 antibody
      • ERp60 antibody
      • ERp61 antibody
      • Glucose Regulated Protein 58 Kd antibody
      • GRP 57 antibody
      • GRP 58 antibody
      • GRP57 antibody
      • HsT17083 antibody
      • p58 antibody
      • PDIA 3 antibody
      • PDIA3 antibody
      • PDIA3_HUMAN antibody
      • Phospholipase C alpha antibody
      • PI PLC antibody
      • Protein disulfide isomerase A3 antibody
      • Protein disulfide isomerase family A member 3 antibody
      • Protein disulfide-isomerase A3 antibody
      see all

    画像

    • Western blot - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
      Western blot - Anti-ERp57 antibody [EPR10678(B)] (ab154191)

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: ERp57 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HepG2 whole cell lysate (20 µg)
      Lane 4: Hek293 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab154191 observed at 57 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab154191 was shown to specifically react with ERp57 in wild-type HAP1 cells as signal was lost in ERp57 knockout cells. Wild-type and ERp57 knockout samples were subjected to SDS-PAGE. ab154191 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)

      Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab154191 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
      Western blot - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
      All lanes : Anti-ERp57 antibody [EPR10678(B)] (ab154191) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 57 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)

      Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling ERp57 with ab154191 at 1/50 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)

      Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154191 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)

      Immunohistochemical analysis of paraffin embedded Human lung carcinoma tissue using ab154191 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)

      Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab154191 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (ab154191)

      Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab154191 showing +ve staining.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-ERp57 antibody [EPR10678(B)] (ab154191)
      Anti-ERp57 antibody [EPR10678(B)] (ab154191)

    参考文献 (2)

    ab154191 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab154191 は 2 報の論文で使用されています。

    • Wang C  et al. Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2. Nat Commun 11:3162 (2020). PubMed: 32572027
    • He Y  et al. Largescale Transcriptomics Analysis Suggests Over-Expression of BGH3, MMP9 and PDIA3 in Oral Squamous Cell Carcinoma. PLoS One 11:e0146530 (2016). IHC-P ; Human . PubMed: 26745629

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