AB10287

Anti-ERp57 抗体

Name: Anti-ERp57 抗体 (ab10287) | Abcam
Brand: Abcam Limited
SKU: AB10287
Price: 83000 JPY
Availability: InStock
Rating: 5 (2 reviews)

Anti-ERp57 antibody

(2 Reviews)

(43 Publications)

Rabbit Polyclonal ERp57 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 43 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Pdia3.

別名を表示する

ERP57, ERP60, GRP58, PDIA3, Protein disulfide-isomerase A3, 58 kDa glucose-regulated protein, 58 kDa microsomal protein, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, Endoplasmic reticulum resident protein 60, p58, ER protein 57, ERp57, ER protein 60, ERp60

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (AB10287)

ICC/IF

AbReview8459****

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (AB10287)

ab10287 at 1/100 staining human HeLa cells by ICC/IF. The cells were premeabilized with digitonin before being blocked and then stained with the antibody for 1 hour. An Alexa-Fluor ® conjugated goat anti rabbit antibody was used as the secondary.

This image is courtesy of an anonymous Abreview

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (AB10287)

Double staining of mouse embryonic fibroblasts (top) and calreticulin deficient fibroblasts (bottom) with rabbit-anti-ERp57 antibodies (1 : 100; upper and lower left panel) and FITC-ConA (upper and lower right panel).

Unknown

Western blot - Anti-ERp57 antibody (AB10287)

Western blot using ab10287 at 1/1000.

Lane 1 – Mouse Embryonic Fibroblasts.
Lane 2 – Calreticulin deficient Mouse Embryonic Fibroblasts.

All lanes:

Western blot - Anti-ERp57 antibody (ab10287) at 1/1000 dilution

Lane 1:

Mouse embryonic cells

Lane 2:

Calreticulin deficient mouse embryomic cells

Predicted band size: 57 kDa

Observed band size: 57 kDa

false

Unknown

Western blot - Anti-ERp57 antibody (AB10287)

All lanes:

Western blot - Anti-ERp57 antibody (ab10287) at 1/1000 dilution

All lanes:

Western blot - Recombinant Human ERp57 protein (<a href='/products/proteins-peptides/recombinant-human-erp57-protein-ab92937'>ab92937</a>) at 0.01 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution

Predicted band size: 57 kDa

true

Exposure time: 150s

Unknown

Western blot - Anti-ERp57 antibody (AB10287)

All lanes:

Western blot - Anti-ERp57 antibody (ab10287) at 1/1000 dilution

All lanes:

Western blot - Recombinant Human ERp57 protein (<a href='/products/proteins-peptides/recombinant-human-erp57-protein-ab92937'>ab92937</a>) at 0.01 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution

Predicted band size: 57 kDa

true

Exposure time: 150s

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

ICC/IF, WB

applications

免疫原

Recombinant Full Length Protein corresponding to Mouse Pdia3.

P27773

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製度

Whole antiserum

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ERp57 also known as 4f9 is a protein responsible for catalyzing disulfide bond formations and isomerizations in the endoplasmic reticulum. ERp57 has a molecular mass of approximately 57 kDa and belongs to the protein disulfide isomerase (PDI) family. Its main expression occurs in the endoplasmic reticulum of cells where it plays a role in ensuring proper protein folding and quality control.

Biological function summary

ERp57 interacts with calnexin and calreticulin to form a complex involved in glycoprotein folding. This complex assists in the proper assembly and maturation of nascent glycoproteins by ensuring correct folding pathways within the cell. ERp57 has a specialized role in managing oxidative protein folding which makes it essential for cell function and homeostasis.

Pathways

ERp57 participates in the calnexin/calreticulin cycle which is critical for protein triage in the endoplasmic reticulum. This cycle ensures that only properly folded proteins progress to their final destinations. ERp57 also interacts with ERp72 another member of the PDI family to facilitate thiol-disulfide exchange reactions. These interactions position ERp57 as a central player in the maintenance of cellular redox balance and protein homeostasis.

Alterations in ERp57 function can relate to neurodegenerative diseases such as Alzheimer's and certain types of cancer. Misfolded proteins often accumulate in these conditions highlighting ERp57's role in preventing cellular stress. Moreover ERp57 associates with the protein tau in Alzheimer's disease where it assists in tau’s proper folding and diminishes its aggregation tendency. Additionally disruptions in the ERp57 pathway may interact with cancer-related proteins like p53 modulating apoptosis and cell cycle regulation.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Protein disulfide isomerase that catalyzes the formation, isomerization, and reduction or oxidation of disulfide bonds in client proteins and functions as a protein folding chaperone (PubMed : 11825568, PubMed : 16193070, PubMed : 27897272, PubMed : 36104323, PubMed : 7487104). Core component of the major histocompatibility complex class I (MHC I) peptide loading complex where it functions as an essential folding chaperone for TAPBP. Through TAPBP, assists the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum. Therefore, plays a crucial role in the presentation of antigens to cytotoxic T cells in adaptive immunity (PubMed : 35948544, PubMed : 36104323).

See full target information PDIA3

style

left-column

文献 (43)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 12: PubMed38964787

2024

Off-the-shelf CAR-NK cells targeting immunogenic cell death marker ERp57 execute robust antitumor activity and have a synergistic effect with ICD inducer oxaliplatin.

Applications

Unspecified application

Species

Unspecified reactive species

Liuhai Zheng,Huifang Wang,Jihao Zhou,Guangwei Shi,Jingbo Ma,Yuke Jiang,Zhiyu Dong,Jiexuan Li,Yuan-Qiao He,Dinglan Wu,Jichao Sun,Chengchao Xu,Zhijie Li,Jigang Wang

Proceedings of the National Academy of Sciences of the United States of America 120:e2219790120 PubMed37307450

2023

Dynamic interactome of the MHC I peptide loading complex in human dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Barends,Nicole Koller,Christian Schölz,Verónica Durán,Berislav Bošnjak,Jennifer Becker,Marius Döring,Hanna Blees,Reinhold Förster,Ulrich Kalinke,Robert Tampé

Communications biology 6:207 PubMed36813842

2023

Nanoscale imaging of CD47 informs how plasma membrane modifications shape apoptotic cell recognition.

Applications

Unspecified application

Species

Unspecified reactive species

Samy Dufour,Pascale Tacnet-Delorme,Jean-Philippe Kleman,Oleksandr Glushonkov,Nicole Thielens,Dominique Bourgeois,Philippe Frachet

iScience 26:105891 PubMed36647387

2023

A KO mouse model for the lncRNA Lhx1os produces motor neuron alterations and locomotor impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Flaminia Pellegrini,Vittorio Padovano,Silvia Biscarini,Tiziana Santini,Adriano Setti,Silvia Giulia Galfrè,Valentina Silenzi,Erika Vitiello,Davide Mariani,Carmine Nicoletti,Giulia Torromino,Elvira De Leonibus,Julie Martone,Irene Bozzoni

Nature communications 13:4701 PubMed35948544

2022

Molecular basis of MHC I quality control in the peptide loading complex.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Domnick,Christian Winter,Lukas Sušac,Leon Hennecke,Mario Hensen,Nicole Zitzmann,Simon Trowitzsch,Christoph Thomas,Robert Tampé

Cell discovery 8:5 PubMed35075146

2022

KRAS(G12D) can be targeted by potent inhibitors via formation of salt bridge.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongwei Mao,Hongying Xiao,Panpan Shen,Yu Yang,Jing Xue,Yunyun Yang,Yanguo Shang,Lilan Zhang,Xin Li,Yuying Zhang,Yanan Du,Chun-Chi Chen,Rey-Ting Guo,Yonghui Zhang

Acta biochimica et biophysica Sinica 53:1216-1226 PubMed34363072

2021

Silencing of ER-resident oxidoreductase PDIA3 inhibits malignant biological behaviors of multidrug-resistant gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Danyang Song,Meng Guo,Kaichu Wu,Jianyu Hao,Yongzhan Nie,Daiming Fan

Scientific reports 11:9080 PubMed33907276

2021

Chemotherapy agents stimulate dendritic cells against human colon cancer cells through upregulation of the transporter associated with antigen processing.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Hsin Liang,Jia-Huei Tsai,Yung-Ming Cheng,Kuang-Yu Chan,Wen-Ling Hsu,Chang-Cheng Lee,Kuo-Hsing Chen,Kun-Huei Yeh

Scientific reports 11:7199 PubMed33785835

2021

In oxygen-deprived tumor cells ERp57 provides radioprotection and ensures proliferation via c-Myc, PLK1 and the AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tobias Ocklenburg,Fabian Neumann,Alexandra Wolf,Julia Vogel,Kirsten Göpelt,Melanie Baumann,Jennifer Baumann,Philip Kranz,Eric Metzen,Ulf Brockmeier

Biochimica et biophysica acta. Molecular basis of disease 1867:166067 PubMed33418034

2021

Cholangiopathy aggravation is caused by VDR ablation and alleviated by VDR-independent vitamin D signaling in ABCB4 knockout mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ester Gonzalez-Sanchez,Haquima El Mourabit,Marion Jager,Marie Clavel,Sophie Moog,Javier Vaquero,Tatiana Ledent,Axelle Cadoret,Jérémie Gautheron,Laura Fouassier,Dominique Wendum,Nicolas Chignard,Chantal Housset

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ERp57 抗体

Anti-ERp57 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (AB10287)

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (AB10287)

Western blot - Anti-ERp57 antibody (AB10287)

Western blot - Anti-ERp57 antibody (AB10287)

Western blot - Anti-ERp57 antibody (AB10287)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製度

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (43)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise