Anti-ERp29 抗体 (ab11420)
Key features and details
- Rabbit polyclonal to ERp29
- Suitable for: IP, Flow Cyt, IHC-Fr, WB, ICC/IF
- Reacts with: Mouse, Rat, Guinea pig, Hamster, Cow, Dog, Human, Non human primates
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-ERp29 antibody
ERp29 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ERp29 -
由来種
Rabbit -
アプリケーション
適用あり: IP, Flow Cyt, IHC-Fr, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Guinea pig, Hamster, Cow, Dog, Human, Non human primates -
免疫原
Recombinant full length protein (His-tag) corresponding to Rat ERp29.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11420の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration. PubMed: 17451556
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-Fr | (1) |
Use at an assay dependent concentration. PubMed: 17652758
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| WB | (3) |
1/2500. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
This antibody detects an 29 kDa protein representing ERp29 in rat liver homogenate. |
| ICC/IF |
1/500.
Immunofluorescence data demonstrates that ERp29 is localized to the ER of rat thyrocytes using ab11420. |
| 特記事項 |
|---|
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IP
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. PubMed: 17451556 ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-Fr
Use at an assay dependent concentration. PubMed: 17652758 |
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WB
1/2500. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). This antibody detects an 29 kDa protein representing ERp29 in rat liver homogenate. |
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ICC/IF
1/500. Immunofluorescence data demonstrates that ERp29 is localized to the ER of rat thyrocytes using ab11420. |
ターゲット情報
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機能
Does not seem to be a disulfide isomerase. Plays an important role in the processing of secretory proteins within the endoplasmic reticulum (ER), possibly by participating in the folding of proteins in the ER. -
組織特異性
Ubiquitous. Mostly expressed in secretory tissues. -
細胞内局在
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 613357 Cow
- Entrez Gene: 10961 Human
- Entrez Gene: 67397 Mouse
- Entrez Gene: 117030 Rat
- Omim: 602287 Human
- SwissProt: P81623 Cow
- SwissProt: P30040 Human
- SwissProt: P57759 Mouse
see all -
別名
- C12orf8 antibody
- Chromosome 12 open reading frame 8 antibody
- Endoplasmic reticulum lumenal protein ERp28 antibody
see all
画像
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Western blot - Anti-ERp29 antibody (ab11420)All lanes : Anti-ERp29 antibody (ab11420) at 1 µg/ml
Lane 1 : MDCK (canine kidney cell line) membrane-enriched extract
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) membrane-enriched extract
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) membrane-enriched extract
Lane 4 : A549 (human lung carcinoma cell line) membrane-enriched extract
Lane 5 : MCF7 (human breast adenocarcinoma cell line) membrane-enriched extract
Lane 6 : A431 (human epidermoid carcinoma cell line) membrane-enriched extract
Lane 7 : HEK-293 (human epithelial cell line from embryonic kidney) membrane-enriched extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 29 kDa12% SDS-PAGE
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Western blot - Anti-ERp29 antibody (ab11420)Western blot of ERp29 in HEK cell lysate using ab11420. -
Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (ab11420)ICC/IF image of ab11420 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11420, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (ab11420)Immunocytochemistry/Immunofluorescence analysis of HMVEC cells labeling ERp29 with ab11420.
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Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (ab11420)Immunocytochemistry/Immunofluorescence analysis of NS-1 cells labeling ERp29 with ab11420.
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Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (ab11420)Immunocytochemistry/Immunofluorescence analysis of p19 cells labeling ERp29 with ab11420.
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Immunocytochemistry/ Immunofluorescence - Anti-ERp29 antibody (ab11420)Immunocytochemistry/Immunofluorescence analysis of rat thyrocytes labeling ERp29 with ab11420.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (19)
ab11420 は 19 報の論文で使用されています。
- Mideksa YG et al. A comprehensive set of ER protein disulfide isomerase family members supports the biogenesis of proinflammatory interleukin 12 family cytokines. J Biol Chem 298:102677 (2022). PubMed: 36336075
- Viviano J et al. ERp29 as a regulator of Insulin biosynthesis. PLoS One 15:e0233502 (2020). PubMed: 32433667
- Bikard Y et al. The KDEL receptor has a role in the biogenesis and trafficking of the epithelial sodium channel (ENaC). J Biol Chem 294:18324-18336 (2019). PubMed: 31653700
- Guo L et al. ERp29 counteracts the suppression of malignancy mediated by endoplasmic reticulum stress and promotes the metastasis of colorectal cancer. Oncol Rep 41:1603-1615 (2019). PubMed: 30569094
- Pergu R et al. The chaperone ERp29 is required for tunneling nanotube formation by stabilizing MSec. J Biol Chem 294:7177-7193 (2019). PubMed: 30877198
