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AB232748

Anti-ERK5 抗体

Anti-ERK5 antibody

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(1 Publication)

Rabbit Polyclonal ERK5 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MAPK7 aa 550-800.

別名を表示する

BMK1, ERK5, PRKM7, MAPK7, Mitogen-activated protein kinase 7, MAP kinase 7, MAPK 7, Big MAP kinase 1, Extracellular signal-regulated kinase 5, BMK-1, ERK-5

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (AB232748)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (AB232748)

Paraffin-embedded human colorectal cancer tissue stained for ERK5 using ab232748 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (AB232748)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (AB232748)

Paraffin-embedded human lung cancer tissue stained for ERK5 using ab232748 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (AB232748)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (AB232748)

Paraffin-embedded human kidney tissue stained for ERK5 using ab232748 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-ERK5 antibody (AB232748)
  • WB

Supplier Data

Western blot - Anti-ERK5 antibody (AB232748)

All lanes:

Western blot - Anti-ERK5 antibody (ab232748) at 2 µg/mL

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Predicted band size: 88 kDa

false

Western blot - Anti-ERK5 antibody (AB232748)
  • WB

Supplier Data

Western blot - Anti-ERK5 antibody (AB232748)

All lanes:

Western blot - Anti-ERK5 antibody (ab232748) at 2 µg/mL

All lanes:

Recombinant human ERK5 protein

Predicted band size: 26 kDa,88 kDa

false

Western blot - Anti-ERK5 antibody (AB232748)
  • WB

Supplier Data

Western blot - Anti-ERK5 antibody (AB232748)

All lanes:

Western blot - Anti-ERK5 antibody (ab232748) at 2 µg/mL

All lanes:

Pig colon tissue lysate

Predicted band size: 88 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Human, Pig

アプリケーション

WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human MAPK7 aa 550-800. The exact immunogen used to generate this antibody is proprietary information.

Q13164

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Immunogen
精製に関する特記事項
ab232748 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
バッファー組成
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ERK5 also known as big MAP kinase 1 (BMK1) is an enzyme that functions as a protein-serine/threonine kinase within the MAP kinase family. It has a molecular weight of approximately 120 kDa. ERK5 is expressed in various tissues including the brain heart and lungs with notable expression in endothelial cells. The protein plays a mechanical role in signal transduction processes which it achieves by transmitting signals from extracellular sources to the cellular nucleus facilitating the regulation of gene expression.
Biological function summary

ERK5 influences cellular functions through its role in promoting cell proliferation and differentiation. It serves as part of a signaling complex that includes the MAP kinase kinase 5 (MEK5). This interaction allows ERK5 to phosphorylate and subsequently activate target substrates such as transcription factors promoting cellular responses necessary for development and survival. ERK5 also plays a role in the response to oxidative stress which contributes to its impact on survival pathways and cellular stress responses.

Pathways

ERK5 acts within the MAPK signaling cascade and is linked to the ERK/MAPK pathway and the phosphoinositide 3-kinase (PI3K) pathway. Within these pathways ERK5 interacts with other MAP kinases and regulatory proteins such as Ras and Raf contributing to a wide range of cellular processes including growth migration and survival. Through these interactions ERK5 influences cellular dynamics and mediates responses to growth factors and stress stimuli.

Abnormal activity of ERK5 has been implicated in various conditions including cardiovascular disease and cancer. Elevated ERK5 signaling can promote tumor progression and metastasis in certain cancers by enhancing tumor cell proliferation and survival. In cardiovascular disorders ERK5 interacts with proteins like calcium/calmodulin-dependent protein kinase II (CaMKII) influencing pathological cardiac remodeling and hypertrophy. These associations highlight ERK5's impact on disease mechanisms and highlight its potential as a therapeutic target.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. As part of the MAPK/ERK signaling pathway, acts as a negative regulator of apoptosis in cardiomyocytes via interaction with STUB1/CHIP and promotion of STUB1-mediated ubiquitination and degradation of ICER-type isoforms of CREM (By similarity). May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction.
See full target information MAPK7
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Nature 637:402-411 PubMed39633051

2024

Fetal hepatocytes protect the HSPC genome via fetuin-A.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Lin Guo,Yi-Ding Wang,Yan-Jun Liu,Lei Chu,Hua Zhu,Ye Hu,Ren-Yan Wu,Hong-Yu Xie,Juan Yu,Shui-Ping Li,Chao-Yang Xiong,Ruo-Yan Li,Fang Ke,Lei Chen,Guo-Qiang Chen,Liang Chen,Fan Bai,Tariq Enver,Guo-Hong Li,Huai-Fang Li,Deng-Li Hong
View all publications
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代替製品

Primary Antibodies

AB196609

Anti-ERK5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK5 antibody (AB196609)

  • 4
  • 1
Primary Antibodies

AB5686

Anti-ERK5 (phospho T219 + Y221) antibody

Western blot - Anti-ERK5 (phospho T219 + Y221) antibody (AB5686)

  • 3
  • 2
Primary Antibodies

AB40809

Anti-ERK5 antibody [EP791Y]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-ERK5 antibody [EP791Y] (AB40809)

  • 5
  • 1
Primary Antibodies

AB232538

Anti-ERK5 antibody [EP791Y] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-ERK5 antibody [EP791Y] - BSA and Azide free (AB232538)

  • 0
  • 0
Primary Antibodies

AB264300

Anti-ERK5 antibody

Immunoprecipitation - Anti-ERK5 antibody (AB264300)

  • 0
  • 0

組合せ製品

Primary Antibodies

AB201015

Anti-ERK1 (phospho T202) + ERK2 (phospho T185) antibody [EPR19401]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-ERK1 (phospho T202) + ERK2 (phospho T185) antibody [EPR19401] (AB201015)

  • 4
  • 4
Primary Antibodies

AB184699

Anti-ERK1 + ERK2 antibody [EPR17526]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-ERK1 + ERK2 antibody [EPR17526] (AB184699)

  • 5
  • 1
Primary Antibodies

AB179461

Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461)

  • 4
  • 8
Primary Antibodies

AB76299

Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (AB76299)

  • 0
  • 0

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