Anti-ERK2 抗体 (ab15282)
Key features and details
- Rabbit polyclonal to ERK2
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-ERK2 antibody
ERK2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ERK2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide within Human ERK2 aa 300 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P63085 -
ポジティブ･コントロール
- A431 cells, Breast carcinoma
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特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab15282の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
IHC-P
1/200. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity. -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
ドメイン
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻訳後修飾
Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5594 Human
- Entrez Gene: 26413 Mouse
- Entrez Gene: 116590 Rat
- Omim: 176948 Human
- SwissProt: P28482 Human
- SwissProt: P63085 Mouse
- SwissProt: P63086 Rat
- Unigene: 431850 Human
see all -
別名
- ERK 2 antibody
- ERK-2 antibody
- ERT1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK2 antibody (ab15282)ab15282, at 1/200 dilution, staining ERK2 in breast carcinoma by IHC-P.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab15282 は 15 報の論文で使用されています。
- Ge W et al. Advanced glycation end products promote osteoporosis by inducing ferroptosis in osteoblasts. Mol Med Rep 25:N/A (2022). PubMed: 35211757
- Ribeiro J et al. Restoration of visual function in advanced disease after transplantation of purified human pluripotent stem cell-derived cone photoreceptors. Cell Rep 35:109022 (2021). PubMed: 33882303
- Varin J et al. Substantial restoration of night vision in adult mice with congenital stationary night blindness. Mol Ther Methods Clin Dev 22:15-25 (2021). PubMed: 34401402
- Ryals RC et al. A Ketogenic & Low-Protein Diet Slows Retinal Degeneration in rd10 Mice. Transl Vis Sci Technol 9:18 (2020). PubMed: 33117609
- Barnea-Cramer AO et al. Repair of Retinal Degeneration following Ex Vivo Minicircle DNA Gene Therapy and Transplantation of Corrected Photoreceptor Progenitors. Mol Ther 28:830-844 (2020). PubMed: 32027843