Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) 抗体 [E337] (ab32538)

Submit a review Q&A (1)References (29)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538)
  • Immunocytochemistry/ Immunofluorescence - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538)
  • Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538)
  • Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538)
  • Flow Cytometry (Intracellular) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538)
  • Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E337] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
  • Suitable for: WB, IHC-P, Flow Cyt (Intra), ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337]
    Erk1 (pT202/pY204) + Erk2 (pT185/pY187) 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [E337] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)

  • 由来種

    Rabbit

  • 特異性

    The antibody detects ERK1 phosphorylated on Threonine 202 and Tyrosine 204 and ERK2 phosphorylated on Threonine 185 and Tyrosine 187.

  • アプリケーション

    適用あり: WB, IHC-P, Flow Cyt (Intra), ICC/IFmore details
    適用なし: IP

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human Erk1 (pT202/pY204) + Erk2 (pT185/pY187). The exact sequence is proprietary.
    (Peptide available as ab205613)

  • ポジティブ･コントロール

    • WB: Serum starved A431 cell lysate treated with EGF. IHC-P: Human thyroid gland cancer tissue. ICC/IF: A431 cells +- EGF.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab32538の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/500 - 1/10000. Detects a band of approximately 42, 44 kDa (predicted molecular weight: 42 , 44 kDa).
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/200 - 1/250.
特記事項
WB
1/500 - 1/10000. Detects a band of approximately 42, 44 kDa (predicted molecular weight: 42 , 44 kDa).
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF
1/200 - 1/250.
追加情報
Is unsuitable for IP.

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538)

    Immunohistochemical analysis of paraffin-embedded human thyroid gland cancer using anti-ERK1(pT202/pY204)/ERK2(pT185/pY187) (ab32538) at dilution of 1:50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538)
    Immunocytochemistry/ Immunofluorescence - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538)

    Immunocytochemistry/Immunofluorescence analysis of 4% paraformaldehyde A431+-EGF(100ng/ml,5min) labelling Erk1 (pT202/pY204) + Erk2 (pT185/pY187) with ab32538 at dilution of 1/200. The secondary antibody used was Alexa Fluor® 488 Goat-Anti-Rabbit IgG (ab150077) at dilution of 1/400. The counter stain was done with DAPI (blue).

  • Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538)
    Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538)
    Lane 1 : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538) at 1/500 dilution
    Lane 2 : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538) at 1/2000 dilution
    Lane 3 : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538) at 1/10000 dilution

    All lanes : A431 treated with EGF for 10 minutes

    Lysates/proteins at 0.1 µg/ml per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 , 44 kDa
    Observed band size: 42.44 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    First-antibody diluted with 1% BSA.

     

  • Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538)
    Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538)

    THP1 cells were incubated at 37°C for 3 minutes with vehicle control (0 μM) and different concentrations of ZK 756326 (ab120814). Increased expression of ERK1 (phospho T202 + Y204 ) + ERK2 (phospho T185 + Y187) (ab32538) in THP1 cells correlates with an increase in ZK 756326 concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab32538 at 1/500 dilution and ab17942 at 1 μg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

  • Flow Cytometry (Intracellular) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538)
    Flow Cytometry (Intracellular) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538)

    Overlay histogram showing HeLa cells stained with ab32538 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32538, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit DyLight® 488 (IgG; H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. 

     

     

  • Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538)
    Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] (ab32538)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (29)

ab32538 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab32538 は 29 報の論文で使用されています。

  • Zoi I  et al. Polycystin-1 and hydrostatic pressure are implicated in glioblastoma pathogenesis in vitro. J Cell Mol Med 26:1699-1709 (2022). PubMed: 35106909
  • Peng M  et al. Programmed death-ligand 1 signaling and expression are reversible by lycopene via PI3K/AKT and Raf/MEK/ERK pathways in tongue squamous cell carcinoma. Genes Nutr 17:3 (2022). PubMed: 35164673
  • Bruno C  et al. Protective effects of the postbiotic deriving from cow's milk fermentation with L. paracasei CBA L74 against Rotavirus infection in human enterocytes. Sci Rep 12:6268 (2022). PubMed: 35428750
  • Cimmino F  et al. FGFR1 is a potential therapeutic target in neuroblastoma. Cancer Cell Int 22:174 (2022). PubMed: 35488346
  • Deng Y  et al. ARV-771 Acts as an Inducer of Cell Cycle Arrest and Apoptosis to Suppress Hepatocellular Carcinoma Progression. Front Pharmacol 13:858901 (2022). PubMed: 35600879
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

Question

Ich hatte Sie gestern angerufen, um Auskunft zu den RabMab Antikörpern gebeten, die gegen humanes ERK gerichtet sind. Sie haben mich aber falsch verstanden, und mir nun die Information zu ERG geschickt.

Ich würde Sie bitten, die Sequenzen der ERK-RabMabs mit der Sequenz aus Drosophila zu vergleichen.

Bitte entschuldigen Sie diese Unannehmlichkeiten.

Mit freundlichen Grüßen

Read More
Answer

Vielen Dank für Ihren Hinweis. Es tut mir Leid, dass ich Sie falsch verstanden habe und um die daraus resultierende Verzögerung.

Ich habe jetzt unserer RabMabs gegen ERK1, ERK1+2 sowie ERK2 durchsucht. Ich habe Ihnen eine komplette Liste zusammengestellt.

1. https://www.abcam.com/erk1--erk2-phospho-t202--y204--t185--y187-antibody-e337-ab32538.html weißt eine 100%ige Übereinstimmung im Immunogen mit Drosophila auf.

2. Anti-ERK2 antibody [E460] (ab32081) weißt eine 60%ige Übereinstimmung und Anti-ERK1 antibody [CT] (ab77759) eine 47.2%ige Übereinstimmung im Immunogen auf. Die Übereinstimmung der Sequenzen ist recht niedrig und wir würden nicht erwarten, dass diese Antikörper das Drosophila Protein detektieren.

3. Anti-ERK1 antibody [Y72] (ab32537), Anti-ERK1 antibody [EP4967] (ab109282) und Anti-ERK1 antibody [Y71] (ab32066) haben keine Übereinstimmung im Immunogen mit dem Drosophila Protein.

Unseres Wissens wurde dieser ab32538 noch nicht in Drosophila getestet. Wenn Sie sich dafür entscheiden diesen Antikörper in Drosophila zu verwenden würde ich Ihnen gerne unseren AbTrial hierfür anbieten. Mit dem AbTrial erhalten Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Der Rabatt würde den ganzen Wert von €345.00 des ab32538 abdecken, und gegen eine erneute Bestellung eines weiteren Produktes von uns verrechnet werden.

Um von diesem Angebot profitieren zu können, folgen Sie bitte den folgenden Schritten:

1.) Bestätigen Sie mir bitte, dass Sie ab32538 in Drosophila testen möchten. Ich werde ihnen dann einen Rabattcode zusenden.

2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

4.) Testen Sie den Antikörper in Drosophila.

5.) Teilen Sie uns das Ergebnis Ihres Tests innerhalb von 4 Monaten durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes".
Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
https://www.abcam.com/abreviews

6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können ein anderes Produkt zu dem gleichen Preis wie ab32538 bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode und die Bestellnummer von ab32538 bereit). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

Die Bedingungen zu unserem 100% AbTrial Rabatt können Sie unter dem folgenden Link nachlesen:
https://www.abcam.com/index.html?pageconfig=resource&rid=11998

Ich hoffe, diese Informationen helfen Ihnen weiter und ich habe diesmal nach dem richtigen Antikörper geschaut. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com