Anti-ERK1 (phospho T202) 抗体 (ab47310)
Key features and details
- Rabbit polyclonal to ERK1 (phospho T202)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ERK1 (phospho T202) antibody
ERK1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ERK1 (phospho T202) -
由来種
Rabbit -
特異性
ab47310 detects endogenous levels of p44/42 MAP Kinase only when phosphorylated at threonine 202. -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human ERK1 aa 150-250 (phospho T202). Swiss-Prot #: P27361/P28482
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ポジティブ･コントロール
- Extracts from A431 cells, EGF-treated.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody was purified using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47310の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 4 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| WB |
1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
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| 特記事項 |
|---|
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IHC-P
Use a concentration of 4 mg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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WB
1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
ターゲット情報
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機能
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
ドメイン
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻訳後修飾
Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204. - Information by UniProt
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参照データベース
- Entrez Gene: 5595 Human
- Entrez Gene: 26417 Mouse
- Entrez Gene: 50689 Rat
- Omim: 601795 Human
- SwissProt: P27361 Human
- SwissProt: Q63844 Mouse
- SwissProt: P21708 Rat
- Unigene: 861 Human
see all -
別名
- ERK 1 antibody
- ERK-1 antibody
- ERK1 antibody
see all
画像
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Western blot - Anti-ERK1 (phospho T202) antibody (ab47310)All lanes : Anti-ERK1 (phospho T202) antibody (ab47310) at 1/500 dilution
Lane 1 : EGF treated A431 cell extract (200ng/ml,30min)
Lane 2 : A431 cell extracts
Predicted band size: 43 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 (phospho T202) antibody (ab47310)ab47310 (4µg/ml) staining ERK1 (Phospho T202) in human colon.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 . Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab47310 は 3 報の論文で使用されています。
- Zhao S et al. Phosphoproteome Reveals Extracellular Regulated Protein Kinase Phosphorylation Mediated by Mitogen-Activated Protein Kinase Kinase-Regulating Granulosa Cell Apoptosis in Broody Geese. Int J Mol Sci 24:N/A (2023). PubMed: 37569653
- Korolkova OY et al. Reciprocal expression of Annexin A6 and RasGRF2 discriminates rapidly growing from invasive triple negative breast cancer subsets. PLoS One 15:e0231711 (2020). PubMed: 32298357
- Tor YS et al. Induction of Apoptosis in MCF-7 Cells via Oxidative Stress Generation, Mitochondria-Dependent and Caspase-Independent Pathway by Ethyl Acetate Extract of Dillenia suffruticosa and Its Chemical Profile. PLoS One 10:e0127441 (2015). WB ; Human . PubMed: 26047480