Anti-ERK1 抗体 [E19] (ab78918)
![Western blot - Anti-ERK1 antibody [E19] (ab78918)](https://www.abcam.co.jp/ps/products/78/ab78918/Images/ERK1-Primary-antibodies-ab78918-2.png "Western blot - Anti-ERK1 antibody [E19] (ab78918)")
Key features and details
- Mouse monoclonal [E19] to ERK1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-ERK1 antibody [E19]
ERK1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [E19] to ERK1 -
由来種
Mouse -
特異性
Recognizes only the ERK1 protein (e.g.: from human and mouse cells) -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human ERK1 aa 1-379.
Database link: P27361 -
ポジティブ･コントロール
- HEK293 and Jurkat cell lysates.
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特記事項
For longer term storage add 0.1% Na Azide.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Constituent: 0.0268% PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
E19 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab78918の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
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| IP |
Use a concentration of 0.5 - 2 µg/ml.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
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IP
Use a concentration of 0.5 - 2 µg/ml. |
ターゲット情報
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機能
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). -
配列類似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
ドメイン
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻訳後修飾
Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204. - Information by UniProt
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参照データベース
- Entrez Gene: 5595 Human
- Omim: 601795 Human
- SwissProt: P27361 Human
- Unigene: 861 Human
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別名
- ERK 1 antibody
- ERK-1 antibody
- ERK1 antibody
see all
画像
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Western blot - Anti-ERK1 antibody [E19] (ab78918)All lanes : Anti-ERK1 antibody [E19] (ab78918) at 1/10000 dilution
Lane 1 : HEK293 cell lysate at 1 µg/ml
Lane 2 : HEK293 cell lysate at 5 µg/ml
Lane 3 : HEK293 cell lysate at 10 µg/ml
Predicted band size: 43 kDa
Observed band size: 43 kDa -
![Immunoprecipitation - Anti-ERK1 antibody [E19] (ab78918)](https://www.abcam.co.jp/ps/products/78/ab78918/Images/ERK1-Primary-antibodies-ab78918-3.png)
Immunoprecipitation - Anti-ERK1 antibody [E19] (ab78918)Detection of ERK1 by Western Blot of Immunprecipitate.
ab78918 at 0.5µg, 1µg, and 2µg (lanes 1-3 respectively) immunoprecipitating ERK1 from 50µg Jurkat cell lysate.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab78918 は論文での使用が確認できていません。
